J. AOAC Int. 94, 735-742 (2011). HPTLC and TLC of ofloxacin on silica gel with ethanol - conc. ammonia 4:1 in a horizontal chamber. Quantitative determination by fluorescence measurement at 313 nm. Simulated samples at a residue level of 1 mg/m² were prepared by spreading the calculated amount of ofloxacin solution on 1, 5, and 10 dm² stainless steel surfaces. The hRf of ofloxacin was 56. The mean recovery (n = 6) was 88.6-95.3 % with a CV of 3.8-4.9 %. The LOD was 0.6 ng/zone and the LOQ was 2 ng/zone, but it was shown that these can be lowered by immersion of the developed plate into a solution of liquid paraffin - n-hexane 1:2 to approximately 0.3 and 0.9 ng/zone. The repeatability (system precision) was 4.2 % for 2 ng, and 3.7 % for 20 ng. The recovery was between 88.6-95.3 %.

Planta Med. 75, 1000 (2009). TLC and HPTLC of Mezereon (Daphne mezereum) bark extracts and scopoletin, umbelliferone, mezerein, and daphnetoxin on silica gel with various mobile phases containing toluene, ethyl acetate, and formic acid at different proportions. Detection under UV 254 and 366 nm and visible light. The applied procedure may be proposed for an updated and optimized TLC identification test of the homeopathic monograph of Daphne mezereum L.

J. Liq. Chromatogr. Relat. Technol. 32, 567-577 (2009). HPTLC of 6-gingerol (1), 8-gingerol (2), 10-gingerol (3), and 6-shogaol (4) in commercial gingers on Lichrosphere silica gel with toluene - ethyl acetate 3:1. Detection by spraying with anisaldehyde-sulfuric acid reagent, followed by heating at 120 ºC for 10 min. Quantitative determination by absorbance measurement at 577 nm. Linearity was between 96-480 ng for (1), 39-196 ng for (2), 49-242 ng for (3) and 50-256 ng for (4). Limits of detection and quantification were 26 and 77 ng/zone for (1), 16 and 47 ng/zone for (2), 17 and 50 ng/zone for (3) and 33 and 99 ng/zone for (4). %RSD of repeatability and intermediate precision were below 5 %. Recoveries were between 99.7 and 104 % for (1)-(4).

J. Planar Chromatogr. 24, 227-231 (2011). TLC of diethylamino hydroxybenzoyl hexyl benzoate (DHHB) and octyl methoxycinnamate (OMC) on silica gel with cyclohexane - diethyl ether - acetone 15:1:2 with chamber saturation for 20 min. Quantitative determination by densitometry in absorbance mode at 300 nm (for OMC) and 360 nm (for DHHB). Linearity was between 200 and 2000 ng/zone both for DHHB and OMC. LOD and LOQ was 50 and 472 ng/zone for DHHB and 50 and 488 ng/zone for OMC. The hRf value was 30 for DHHB and 47 for OMC. Recovery of DHHB was between 97.8-101.9 % and of OMC between 98.8-102.7 %

CBS 107, 11-12 (2011). HPTLC of tiliroside on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:27 + 1 % heptane. For quantification determination by densitometry in absorbance mode at 315 nm. For side component analysis detection by spraying with natural products reagent and evaluation under UV 366 nm, and by spraying with anisaldehyde reagent followed by heating for 15 min at 90-125 °C and evaluation under white light. The presence of relevant side components (e.g., coumaric acid, kaempferol and glucose) could be excluded.

Chinese J. of Anal. Chem. (Fenxi Huaxue) 39 (9), 1427-1431 (2011). Description of a method for determination of residual glycerides in biodiesel by TLC-thermally assisted hydrolysis and methylation GC (TLC-THM-GC) with a pyrolysis-GC system. The residual glycerides were determined based on the total peak area of the fatty acid methyl esters formed. TLC of glycerides on silica gel with toluene - acetone 23:2. Detection by exposure to iodine vapor. The zones of interest were scraped off the plate and extracted with ethyl acetate. GC quantification of the glycerides after methylating the mixture of 3 µL methanolic trimethylsuIfonium hydroxide (0.1 mol/L) and 3 µL sample extract at 350 °C. In the presence of organic alkali and trimethylsuIfonium hydroxide the glycerides are converted into their corresponding fatty acid methyl esters. The linearity for monoglyceride, diglyceride and triglyceride was between 60-2000 mg/L, with %RSD of 3.2-7.2 % at a level of 250 mg/L, the regression coefficients were between 0.9863-0.9993. The proposed method was successfully applied for the determination of the content of residual glycerides and the composition of fatty acids in biodiesel samples produced from rape oil and palm oil.

J. Liq. Chromatogr. Relat. Technol. 34, 920-927 (2011). TLC of flumequine in milk on silica gel with dichloromethane - methanol - 2-propanol - 25 % aqueous ammonia 3:3:5:2. Bioautography by dipping into an Escherichia coli bacteria suspension for 5 h at 37 ºC. After incubation, the plates were sprayed with 0.2 % MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide] aqueous solution and incubated for about 30 min at 37 ºC. The new procedure gave better recoveries of flumequine residues from milk compared with the previous method.

J. Planar Chromatogr. 24, 218-221 (2011). HPTLC of alfuzosin hydrochloride (ALF) and dutasteride (DUTA) in the bulk drug and in a tablet formulation on silica gel with toluene - methanol - dichloromethane 6:1:1 + 1 drop triethylamine. Quantitative determination by densitometry at 247 nm. The hRf of ALF was 46 and of DUTA 65. Linearity was between 300-600 ng/band for ALF and 500-100 ng/band for DUTA. LOD and LOQ were 100 and 200 ng/band for ALF and 300 and 400 ng/band for DUTA. Precisions (%RSD) for repeatability of application were 1.8 and 1.5 % for ALF and 1.5 and 1.4 % for DUTA. The inter-day and intra-day precision (%RSD, n = 6) was 1.0 and 0.9 % for ALF and 1.7 and 0.8 % for DUTA, respectively. Recovery (by standard addition) was between 98.9-101.6 % for both compounds.