Chinese J. of Ethnomed. & Ethnopharm. 23, 51-52 (2012). Shengjing Yusi capsule is a herbal TCM preparation for treating male infertility. For quality control, TLC on silica gel 1) for Epimedium davidii Franch, with chloroform – methanol – water 13:7:2, detection under UV 366 nm; 2) for Fructus Schisandrae Chinensis, with petroleum ether (30-60°C) – ethyl acetate – formic acid 15:5:1, detection by spraying with 5 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible in daylight.

J. Planar Chromatogr. 26, 491-495 (2013). TLC of enantiomers of (±)-bupropion on silica gel, impregnated with L-Glu, with acetonitrile - methanol - dichloromethane - water 28:5:11:5. Detection by exposition to iodine vapor. LOD was 0.2 µg/zone for each enantiomer.

CBS 111, 5-6 (2013). HPTLC of caffeine, taurine, and arginine in shampoo samples extracted with isopropanol, on silica gel over 50 mm with isopropanol - n-heptane - water 7:3:1 for caffeine and isopropanol - water 4:1 for arginine and taurine. Detection under UV 254 nm (caffeine) and after spraying with ninhydrin reagent under white light (arginine and taurine). Quantitative absorbance measurement at UV 254 nm for caffeine and UV 600 nm for arginine and taurine. The hRF of caffeine was 54. Precision (%RSD) for the polynomial calibration of caffeine was 3.9 % (n=3). The hRf of taurine was 24. Arginine remained at the start position under these conditions. The content of taurine and caffeine found in shampoos corresponded to the usual amount of 0.1 % active ingredient in a formulation.

J. Strait Pharm. 26 (3), 86-87 (2014). Zhibai Dihuang Wan pill is a herbal TCM for treatment of insomnia, dizziness, irritability, spermatorrhea, constipation, dry mouth and sore throat, tinnitus, hectic fever and night sweat, etc. For quality control, HPTLC for simultaneous identification of Alisma plantago-aquatica Linn., Poria cocos (Schw.) Wolf and the standard ursolic acid on silica gel with petroleum ether (60-90 °C) – ethyl formate – formic acid 15:5:1, detection 1) under UV 366 nm, 2) by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight and under UV 366 nm. The method was used for the analysis of 18 products manufactured by 17 pharmaceutical companies factories and was found simple, specific, reproducible, robust and well suitable for the purpose.

Trends in Chromatography 8, 131-135 (2013). Based on the Minilab TLC screening methods two individual and one simultaneous HPTLC methods for quantification of lumefantrine and artemether were developed. HPTLC of artemether, lumefantrine, and both substances in a combined tablet formulation on silica gel with ethyl acetate - glacial acetic acid - toluene 2:1:9 with chamber saturation. Quantitative absorbance measurement of lumefantrine at 254 nm. For artemether, detection by spraying with methanol - 96 % sulfuric acid 19:1 and heating at 100 °C for 5 minutes, evaluation in daylight and quantitative absorbance measurement at 610 nm. With the simultaneous method artemether zones were detected by heating at 160 °C for 5 min, and after cooling absorbance measurement at 254 nm was performed. This is a novel, reagent-free detection method based on thermochemical activation of fluorescence quenching. Precision (%RSD) for the artemether individual method was 0.1-0.6 % and recovery 98-103 %. Precision (%RSD) for the lumefantrine individual method was 0.9-1.8 % and recovery 100-102 %. With the simultaneous method the hRF was 34 for lumefantrine and 61 for artemether, precision (%RSD) was below 3 % and recovery was 98-100 %.

Chinese J. of Inform. on TCM 20 (8), 56-58 (2013). Zaodi Anshen Wan pill is herbal TCM for treatment of neurasthenia, fatigue syndrome, depression, insomnia, dizziness and tinnitus, palpitation, etc. For quality control, HPTLC on silica gel 1) for Ziziphus jujuba Mill. var. spinosa, with water-saturated n-butanol, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4, evaluation under UV 366 nm; 2) for Radix polygoni multiflori preparata and the standards emodin and physcion, with petroleum ether (30-60 ˚C) – ethyl formate – formic acid 15:5:1, detection by exposure to ammonia vapors, evaluation under UV 366 nm. Analysis of Schisandra chinensis, schisandrin, deoxyschizandrin and gamma-schizandrin B by HPLC.

J. Planar Chromatogr. 27, 409-415 (2014). TLC of propanolol enantiomers on silica gel impregnated with 15 nm gold nanoparticles (AuNPs) and 10.0 mM L-cysteine (L-cys) pH = 8.1, as first and second layer, respectively and developed with 10.0 mM copper(II) acetate, in 70% ethanol – water adjusted at pH 6.3.

leaves by high-performance thin-layer chromatography. J. Planar Chromatogr. 27, 357-361 (2014). HPTLC of (1) lawsone, (2) methylene-3,3'-bilawsone, and (3) lawsone methyl ether on silica gel with n-butyl acetate – chloroform – glacial acetic acid 60:40:1. Quantitative determination by absorbance measurement at 280 nm. The hRF values for (1) to (3) were 23, 15 and 36, respectively. Linearity was between 5 and 15 μg/zone for each substance. The intermediate intra-day and inter-day precisions were below 2 % (n=3). The LOD and LOQ were 200 and 600 ng/zone for (1) to (3), respectively.