J. AOAC Int. 99, 374-379 (2016). HPTLC of glabridin in the roots of Glycyrrhiza glabra on silica gel with hexane – ethyl acetate – chloroform 5:4:3. Quantitative determination by absorbance measurement at 285 nm. The hRF value of glabridin was 48. Linearity was in the range of 50-500 ng/zone. The LOD and LOQ were 10 ng/zone and 30 ng/zone, respectively. Intermediate precisions were below 2 %. Recovery was between 100.6 and 102.0 %. Compared to a validated HPLC method there was no statistically significant difference in the mean values.

Malaria J. 15, 215 (2016). TLC of methanol extracted tablets and the standards sulfadoxine and pyrimethamine with the GPHF Minilab (procedure for sulfadoxine) on silica gel with ethyl acetate – methanol 3:1 for approximately 15 min. Detection under UV 254 nm, and by exposure to iodine vapor. Documentation with a commercial digital camera. For comparison to authentic paracetamol and co-trimoxazole samples, the GPHF Minilab solvent systems for paracetamol (acetone – toluene – acetic acid 20:20:1), pyrimethamine (ethyl acetate – methanol – acetone – conc. aqueous ammonia 24:12:4:1) and co-trimoxazole (ethyl acetate – methanol 3:1) were used. 28 market samples were collected and analyzed, 10 samples were further analyzed by HPLC according to the USP. One sample was falsified, the box contained a mixture of paracetamol tablets and co-trimoxazole tablets and none of the tablets contained any anti-malarial drugs.

Chinese J. of Inform. on TCM 20 (10), 46-48 (2013). Buxin Ruanmai granule is a herbal TCM for coronary heart disease and angina pectoris. For quality control, TLC (1) for Ophiopogon japonicus, on silica gel with n-hexane – ethyl acetate – formic acid 10:10:4, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm; (2) for common Macrocarpium fruit, on silica gel with ethyl acetate – ethanol – glacial acetic acid 50:10:1, detection by spraying with 5 % vanillin-sulfuric acid in ethanol 1:4 and heating at 105 °C until the zones are visible; (3) for Whitmania pigra Whitman, on silica gel with cyclohexane – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm; (4) for Ginkgo biloba, on polyamide layer with 40 % acetic acid, detection by spraying with 3 % aluminium trichloride in ethanol, drying in a warm stream of air, evaluation under UV 366 nm; (5) for Polygonum cuspidatum Sieb. et Zucc., on silica gel with petroleum ether (60-90 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm. Quantitative determination of salidroside and loganin by HPLC.

J. Ethnopharmacol. 192, 283-291 (2016). Ubtan is a traditional herbal formulation in the Indian system of medicine, prepared by mixing powdered turmeric (Curcuma longa L.), chickpea (Cicer arietinum L.) and sandal wood (Santalum album L.). HPTLC of four in-house formulations of Ubtan (UF-1, UF-2, UF-3 and UF-4, prepared with varying quantities of each ingredient) on silica gel with cyclohexane – ethyl acetate – formic acid 90:9:1. Detection by spraying with p-anisaldehyde sulfuric acid reagent, followed by drying at 40 ºC for 10 min. The hRF values for UF-1 were 70 and 42.

Food Control. 68, 220-228 (2016). HPTLC of biogenic amines (putrescine, histamine, tyramine and agmatine) in microbial cultures on silica gel with chloroform – benzene – triethylamine 12:9:2. Quantitative determination by absorbance measurement at 254 nm.

J. Planar Chromatogr. 29, 380-387 (2016). HPTLC of metolazone (1) and spironolactone (2) on silica gel with ethyl acetate ‒ chloroform ‒ glacial acetic acid 50:50:1. Quantitative determination by absorbance measurement at 238 nm. The hRF values for (1) and (2) were 51 and 79, respectively. Linearities were between 50 and 300 ng/zone for (1) and 200 and 1200 ng/zone for (2). The intermediate precisions were below 1.3 % (n=6). The LODs and LOQs were 2 and 5 ng/zone for (1) and 5 and 16 ng/zone for (2). Average recoveries were 99.7 % for (1) and 99.7 % for (2). The developed method successfully separated drug substances from degradation products formed under various stress conditions.

Rev. Bras. Farmacogn. 27, 50-53 (2017). HPTLC of quercetin (1) and gallic acid (2) in Leea indica on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 63 and 45, respectively. Linearity was between 200 and 1000 ng/zone for (1) and between 200 and 1200 ng/zone for (2), respectively. The intermediate precision was <2 % (n=3). The LOD and LOQ were 21 and 65 ng/zone for (1) and 15 and 55 ng/zone for (2). Recovery rate was in the range of 98.0-99.1 % for (1) and 99.3-100.2 % for (2).

J. Planar Chromatogr. 30, 251-258 (2017). HPTLC of piperine in black pepper on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid 14:6:1. Quantitative determination by absorbance measurement at 280 nm. The hRF value for piperine was 40. Linearity was between 100 and 900 ng/zone. LOD and LOQ were 20 and 60 ng/zone, respectively. The intermediate precision was <2 % (n=6). Recovery ranged from 99.2 to 100.2 %.