Chinese J. of Med. Guide 11 (16), 509-510 (2013). Zhixie Baotong Keli granules are a herbal TCM preparation for the treatment of dysentery, abdominal pain, dry mouth, nausea, and vomiting. For quality control, TLC on silica gel 1) for Tetradium ruticarpum, with cyclohexane – ethyl acetate – methanol – triethylamine 15:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 4 min and viewing under UV 366 nm; 2) for Pogostemon cablin, with petroleum ether (60-90 °C) – ethyl acetate 19:2, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 3) for Fructus Amomi and the standard bornyl acetate, with cyclohexane – ethyl acetate 22:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight.

Chinese J. of Pharm. Practice 31 (3), 228- 230 (2013). Fufang Hanfangji Keli granule is a herbal TCM preparation for treatment of acute and chronic hepatitis, fatty liver, gallbladder disease, and for reducing high total serum cholesterol and aortic atherosclerotic plaques. For quality control, the key drug of the preparation, Salvia miltiorrhiza Bunge, is analysed based on its content of tanshinone IIA. TLC on silica gel with petroleum ether (60-90 °C) – ethyl acetate 4:1. Quantification of tanshinone IIA by HPLC.

J. Planar Chromatogr. 27, 69-71 (2014). HPTLC of bergenin (1) and gallic acid (2) in the rhizome of three Bergenia species, B. ciliata, B. stracheyi, and B. ligulata on silica gel with toluene - ethyl acetate - formic acid 7:11:2. Quantitative determination by absorbance measurement at 314 nm. The hRF values for (1) and (2) were 25 ad 59, respectively.

J. Liq. Chromatogr. Relat. Technol. 37, 1114-1132 (2014). HPTLC of rosuvastatin on silica gel with chloroform - n-hexane - methanol - glacial acetic acid 80:100:40:15:1. Quantitative determination by absorbance measurement at 295 nm. The hRf value for rosuvastatin was 9. Degradation products (DPs) were characterized by ESI-MS. For acidic hydrolysis, 4 major DPs with hRF values of 16, 27, 74 and 77 were obtained. For alkaline hydrolysis, one major DP of hRF 73 was detected. For thermal hydrolysis two major DPs with hRF 74 and 77 were detected. For exposure to hydrogen peroxide, 5 major DPs with hRF 15, 25, 30, 71 and 77 were obtained. For photolytic hydrolysis, 2 major DPs with hRF 70 and 78 were detected. Linearity was in the range of 500-20000 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 170 and 500 ng/zone. Average recovery was 99.9 %.

J. of Xinjiang Trad. Chinese Med. 31 (4), 77-79 (2013). Conger Pian tablet is a herbal TCM for treating sensorineural hearing loss. For quality control, TLC on silica gel 1) for Ligusticum chuanxiong and the standard ferulic acid, with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 2) for Cornus officinalis Sieb. et Zucc. and the standard ursolic acid, with cyclohexane – chloroform - ethyl acetate 20:5:8, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ˚C until the zones are visible in daylight. Quantification of icariin by HPLC.

J. Liq. Chromatogr. Relat. Technol. 37, 1568-1582 (2014). HPTLC of artesunate (1) and amodiaquine (2) on silica gel with acetonitrile – water – ammonia 40:7:2. Quantitative determination by absorbance measurement at 366 nm. The hRf values for (1) and (2) were 44 and 50, respectively. Linearity was in the range of 100-600 ng/zone for (1) and 50-300 ng/zone for (2). The intermediate/interday/intra-day precisions were below 2 % (n=9). The LOD and LOQ were 25 and 60 ng/zone for (1) and 12 and 30 ng/zone for (2), respectively. Recovery was 98.1-99.9 % for (1) and 99.3-100.9 % for (2).

Chinese J. of Inform. on TCM 20 (1), 67-69 (2013). Zhige Jiejiu Keli granule is a herbal TCM for the prevention and treatment of alcoholism. For quality control, TLC on silica gel 1) for Lobed Kudzuvine root and Pueraria lobata, with the upper phase of toluene – ethyl acetate – formic acid 5:3:1, detection under UV 366 nm; 2) for Hovenia acerba Lindl., with the upper phase of n-hexane – ethyl acetate – ethanol – ammonia 14:8:2:1, detection under UV 366 nm. Quantification of chlorogenic acid by HPLC.

(L.) Merrill. J. Planar Chromatogr. 28, 48-53 (2015). HPTLC of isoflavones daidzein (1) and genistein (2) in Glycine max. on silica gel with chloroform - methanol 20:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values of (1) and (2) were 26 and 42, respectively. Linearity was between 1 and 15 μg/mL. The intermediate intra-day and inter-day precisions were below 2 % (n=6). The LOD and LOQ were 130 and 430 ng/zone for (1) and 300 and 930 ng/zone for (2), respectively. Average recoveries for (1) and (2) were 97.5 % and 96.3 %, respectively.