J. Liq. Chromatogr. Relat. Technol. 38, 1104-1108 (2015). HPTLC of trans-resveratrol on silica gel with ethyl acetate - cyclohexane - n-butanol 9:9:2. Detection through chemiluminescence by dipping into a TCPO solution (250 mg bis(2,4,6-trichlorophenyl)oxalate in 36 mL n-butyl acetate, followed by adding 0.4 mL hydrogen peroxide that was vigorously shaken with the solution for 20 min). The HPTLC plate was covered by a glass plate and measured for 2 min using a very light-sensitive CCD camera. The hRF value for trans-resveratrol was 78. Linearity was in the range of 20-500 ng/zone. LOD and LOQ were 13.7 and 20.3 ng/zone, respectively.

Rev. Bras. Farmacogn. 24, 381-388 (2014). HPTLC fingerprint of Hypochaeris radicata (1) and Taraxacum officinale (2) extracts from roots on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Detection by spraying with vanillin-sulfuric acid reagent followed by visualization under white light. The hRF values of 69 ad 77 were unique to the root of H. radicata, whereas an hRF value of 74 was unique for T. officinale._x000D_

Trends Anal. Chem. 76, 60-70 (2016). The review discusses suitable analytical approaches for the analysis of counterfeit and substandard pharmaceuticals in Tanzania. The authors highlight the importance of TLC and HPTLC for the quality control of pharmaceuticals in developing countries, having a repeatability and a reproducibility of the results comparable to those obtained with HPLC.

J. Sep. Sci. 38, 4180-4186 (2015). HPTLC of glycyrrhizic acid in the dried root and rhizoma of the plants Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, and Glycyrrhiza glabra L. on silica gel with ethyl acetate - water - acetic acid - formic acid 14:2:2:1. Detection by spraying with 10 % solution of sulfuric acid in ethanol, followed by heating at 105 °C for 10 min. Quantitative determination by absorbance measurement at 365 nm.

J. Chromatogr. Sci. 53 (5), 816-823 (2015). HPTLC of ursolic acid, betulinic acid, stigmasterol and lupeol in Shankhpushpi botanicals on silica gel in a twin-trough glass chamber with petroleum ether - ethyl acetate - toluene 7:2:1, detection by spraying with anisaldehyde reagent. Densitometric evaluation at 580 nm. The hRF value of ursolic acid was 21, of betulinic acid 29, of stigmasterol 33 and of lupeol 50. Linearity was between 100-600 ng/zone for all substances. Results of the analysis of real life samples: 134.2 mg/g and 146.1 mg/g ursolic acid in Clitorea ternatea (CT) and Canscora decussata (CD); 110.6 mg/g betulinic acid in Evolvulus alsinoides (EA); 92.8 mg/g, 154.9 mg/g, 31.9 mg/g and 39.2 mg/g stigmasterol in EA, Convolvulus pluricaulis, CT and CD; 30.1 mg/g lupeol in CT.

J. Food Comp. Anal. 49, 94-101 (2016). HPTLC of chlorogenic acid (1), 3,4-dicaffeoylquinic acid (2), 4,5-dicaffeoylquinic acid (3), and 3,5-dicaffeoylquinic acid (4) in 295 varieties and breeding lines of sweet potatoes on silica gel with ethyl acetate – methanol – acetic acid – formic acid – water 27:2:2:2:2. Quantitative determination by absorbance measurement at 330 nm. The hRF values for (1) to (4) were 55, 85, 87 and 95, respectively. Intermediate precisions were below 3.4 %.

J. Ethnopharmacol. 189, 186-193 (2016). HPTLC fingerprinting of salannin in the leaves of neem (Azadirachta indica A. Juss.; Meliaceae) on silica gel with ethyl acetate – dichloromethane – acetic acid – formic acid – water 100:25:10:10:11. The plates were allowed to dry at 100 °C for 5 min and then derivatized with Natural Product reagent (NPR) (1 g diphenyl borinic acid amino ethyl ester in 200 mL of ethyl acetate), followed by heating at 100 °C for 2–3 min and then dipped into anisaldehyde-sulfuric acid reagent (1 mL p-anisaldehyde, 10 mL sulfuric acid, 20 mL acetic acid in 170 mL methanol) and dried at 120 °C for 5 min. Qualitative identification under UV light at 254 or 366 nm.

Chinese J. of Inform. on TCM 20 (7), 59-61 (2013). Shengxinfa Jiaonang capsule is a herbal TCM preparation for treatment of hair loss caused by blood deficiency and kidney essence deficiency, scalp pruritus, alopecia areata, and postpartum alopecia. For quality control, TLC on silica gel (1) for Fallopia multiflora (Thunb.) Harald, with toluene – ethyl acetate – formic acid 15:2:1, detection in daylight; (2) for Psoralea corylifolia Linn., with n-hexane – ethyl acetate 4:1, detection by spraying with 10 KOH in methanol and viewing under UV 366 nm; (3) for Gastrodia elata Bl., with chloroform – ethyl acetate 1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible. Quantification of 2,3,5,4'-tetrahydroxy stilbene-2-Ο-β-D-glucoside by HPLC.