J. Planar Chromatogr. 26, 502-507 (2013). TLC of metal dithiocarbamates: antimonic(III) dipentyldithiocarbamate (1), zinc dipentyldithiocarbamate (2), zinc dibutyldithiocarbamate (3), ferric(III) dipentyldithiocarbamate (4) and lead(II) dipentyldithiocarbamate (5) on RP-18 with 2-propanol - water 10:1. Detection by spraying with sodium azide 2 % - potassium iodide 0.01 M - starch solution 1 % pH 6.0 and exposure to iodine vapour for 15 s. Linearity for compounds (1) to (5) ranged 100-2000, 50-1000, 50-1000, 50-1000 and 100-3000 pmol/zone, respectively. LOD was in the range of 10 pmol/zone for the compounds.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 19 (4), 70-71 (2013). Pothos chinensis (Raf.) Merr. and three other medicinal herbs are used in traditional Yao medicine, they have anti venom, anti tumor, and antioxidant effects and reduce blood sugar. For quality control, TLC of 1) Pothos chinensis (Raf.) Merr., on polyamide phase with acetone – water 1:2, detection by spraying with 2 % aluminiumchloride in ethanol and heating at 105°C until the zones are visible in daylight, and under UV 366 nm; 2) for Mappianthus iodoides Hand.-Mazz., on silica gel with petroleum ether (60-90°C) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight, and under UV 366 nm; 3) Zanthoxylum nitidum (Roxb.) DC., on silica gel with chloroform – methanol – ammonia 150:5:1, detection under UV 366 nm; 4) for Saussurea woodiana Hemsl., on silica gel with petroleum ether (60-90°C) – ethyl formate – formic acid 75:25:2, detection under UV 366 nm.

in Shanhaidanpian tablets) (Chinese). J. of China Pharm. 26 (9), 997-999 (2012). Shanhaidanpian tablets are a herbal TCM preparation for the treatment of poor blood flow, blood stasis and chest pain syndrome. For quality control, TLC on silica gel with toluene – ethyl acetate – formic acid 20:2:1, detection by exposure to vapors until the zones are visible in daylight. Quantitative determination of 2,3,5,4’-tetrahydroxystilbene-2-O-beta-D-glucoside by HPLC.

Chinese J. of Med. Guide 10 (20), 99-102 (2013). Fufang Xiaoji Zhike Milian Gao ointment is herbal TCM preparation for clearing the body heat caused by inflammation, resolving phlegm and relieving asthma. For quality control, TLC on silica gel 1) for Pericarpium Citri Reticulatae and the standard hesperidin, first over 3 cm with ethyl acetate – methanol – water 99:17:13 and thenover 8 cm with toluene – ethyl acetate – formic acid – water 95:45:5:3, detection by spraying with 2 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Schisandra Chinensis and the standard schizandrin A, with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm; 3) for Crataegus pinnatifida and the standard ursolic acid, with toluene – ethyl acetate – formic acid – water 39:8:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 80 °C, detection under UV 366 nm. Quantification of tenuifolin by HPLC.

J. Planar Chromatogr. 27, 204-209 (2014). HPTLC of naringin in the leaves and stems of Rumex vesicarius on silica gel with ethyl acetate - glacial acetic acid - methanol - water 30:10:5:1. Quantitative determination by absorbance measurement at 275 nm. The hRF value for naringin was 46. Linearity was in the range of 100-1000 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=6). The LOD and LOQ were 37 and 111 ng/zone, respectively. The average recovery was 99.5 %.

J. Planar Chromatogr. 27, 2980-2988 (2014). HPTLC of piperine in commercial samples of pepper on silica gel with acetone - n-hexane 3:2. Quantitative determination by absorbance measurement at 360 nm. The hRF value for piperine was 90. The LODs and LOQs were 590 and 1800 ng/zone, respectively. Recoveries were between 98.1 and 99.2 %

J. of China Pharm. 22 (13), 25-27 (2013). Yushang Jiegu Dan pill is a herbal TCM for treating various kinds of body damage, local swelling and pain, early fracture, etc. For quality control, HPTLC 1) for Scutellaria baicalensis Georgi and the standard baicalin, on polyamide layer with toluene – ethyl acetate – methanol – formic acid 10:3:1:2, detection under UV 366 nm; 2) for Rheum palmatum L. and the standards physcion and emodin, on silica gel with toluene – ethyl acetate – formic acid 15:2:1, detection under UV 366 nm; 3) for Caesalpinia sappan L., on silica gel with dichloromethane – acetone – formic acid 10:4:1, detection in daylight; 4) for Paeonia veitchii and the standard paeoniflorin, on silica gel with chloroform – ethyl acetate – methanol - formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 5) for Boswellia carteri, on silica gel with cyclohexane – ethyl acetate 9:1, detection by spraying with 1 % p-dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:50 and heating at 105 °C until the zones are visible in daylight.

Chinese J, Mod. Drug Appl. 7 (18), 13-14 (2013). Ganoderma lucidum is a class of medicinal fungi considered by TCM physicians as the “god medicine” effective for inhibiting tumors, reducing free radicals, slowing down aging, improving the immunity, and having anti inflammation, hypolipidemic and hypoglycemic effects. Ganoderma lucidum polysaccharide is one of the most effective compounds of Ganoderma lucidum. Compared to GC and HPLC, this TLC method has the advantage of simpler sample preparation and multiple sample analysis which makes it more feasible. TLC of the hydrolysate of the sample and of standards glucose, L-rhamnose monohydrate, galactose, mannose and glucuronic acid on microcrystalline cellulose phase, with n-butanol – ethyl acetate – pyridine – water 6:1:5:4 (optimized from 6 different mobile phase systems), first run over 2 cm and second run over 8 cm. Detection by spraying with a solution of o-phthalic acid – n-butanol saturated with water – aniline 16:1000:9 and heating at 110 °C for 5 min and viewing in daylight.