J. Planar Chromatogr. 28, 30-35 (2015). HPTLC of lupeol in the leaves of F. carica, F. nitida, F. ingens, F. palmata, and F. vest, on silica gel with toluene – methanol 9:1. Detection by spraying with p-anisaldehyde reagent, followed by heating. Quantitative determination by absorbance measurement at 540 nm. The hRF value of lupeol was 32. Linearity was between 100 and 800 ng/zone. The intermediate intra-day and inter-day precisions were below 2 % (n=6). The LOD and LOQ were 31 and 94 ng/zone, respectively. Recoveries were in the range of 99.2-99.7 %.

J. Planar Chromatogr. 27, 372-376 (2014). For quality control, HPTLC of cefprozil monohydrate on silica gel with ethyl acetate – acetone – methanol – water – glacial acetic acid 15:5:5:3:1. Quantitative determination by absorbance measurement at 280 nm. The hRF value of cefprozil monohydrate was 20. Linearity was between 200 and 5000 ng/zone. The intermediate precision was below 1.5 % (n=3). The LOD and LOQ were 52 and 75 ng/zone, respectively. Recoveries were in the range of 98.7-101.2 %.

J. AOAC Int. 97, 791-797 (2014). HPTLC of (1) atorvastatin calcium and (2) olmesartan medoxomil on silica gel with chloroform – methanol – acetonitrile 2:1:2. Quantitative determination by absorbance measurement at 276 nm. The hRF values of (1) and (2) were 47 and 69, respectively. Linearity was between 300 and 750 ng/zone for (1), and between 600 and 1500 ng/zone for (2). The intermediate inter-day and intra-day precisions were below 1 % (n=9) for both, (1) and (2). The LOD and LOQ were 6.3 and 19.0 ng/zone for (1) and 3.1 and 9.5 ng/zone for (2). Recoveries for (1) and (2) were in the range of 98-102 %. The method showed comparable results to a validated HPLC method and a UV spectrophotometric method.

J. Liq. Chromatogr. Relat. Technol. 38, 1104-1108 (2015). HPTLC of trans-resveratrol on silica gel with ethyl acetate - cyclohexane - n-butanol 9:9:2. Detection through chemiluminescence by dipping into a TCPO solution (250 mg bis(2,4,6-trichlorophenyl)oxalate in 36 mL n-butyl acetate, followed by adding 0.4 mL hydrogen peroxide that was vigorously shaken with the solution for 20 min). The HPTLC plate was covered by a glass plate and measured for 2 min using a very light-sensitive CCD camera. The hRF value for trans-resveratrol was 78. Linearity was in the range of 20-500 ng/zone. LOD and LOQ were 13.7 and 20.3 ng/zone, respectively.

Rev. Bras. Farmacogn. 24, 381-388 (2014). HPTLC fingerprint of Hypochaeris radicata (1) and Taraxacum officinale (2) extracts from roots on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Detection by spraying with vanillin-sulfuric acid reagent followed by visualization under white light. The hRF values of 69 ad 77 were unique to the root of H. radicata, whereas an hRF value of 74 was unique for T. officinale._x000D_

Trends Anal. Chem. 76, 60-70 (2016). The review discusses suitable analytical approaches for the analysis of counterfeit and substandard pharmaceuticals in Tanzania. The authors highlight the importance of TLC and HPTLC for the quality control of pharmaceuticals in developing countries, having a repeatability and a reproducibility of the results comparable to those obtained with HPLC.

J. Sep. Sci. 38, 4180-4186 (2015). HPTLC of glycyrrhizic acid in the dried root and rhizoma of the plants Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, and Glycyrrhiza glabra L. on silica gel with ethyl acetate - water - acetic acid - formic acid 14:2:2:1. Detection by spraying with 10 % solution of sulfuric acid in ethanol, followed by heating at 105 °C for 10 min. Quantitative determination by absorbance measurement at 365 nm.

J. Chromatogr. Sci. 53 (5), 816-823 (2015). HPTLC of ursolic acid, betulinic acid, stigmasterol and lupeol in Shankhpushpi botanicals on silica gel in a twin-trough glass chamber with petroleum ether - ethyl acetate - toluene 7:2:1, detection by spraying with anisaldehyde reagent. Densitometric evaluation at 580 nm. The hRF value of ursolic acid was 21, of betulinic acid 29, of stigmasterol 33 and of lupeol 50. Linearity was between 100-600 ng/zone for all substances. Results of the analysis of real life samples: 134.2 mg/g and 146.1 mg/g ursolic acid in Clitorea ternatea (CT) and Canscora decussata (CD); 110.6 mg/g betulinic acid in Evolvulus alsinoides (EA); 92.8 mg/g, 154.9 mg/g, 31.9 mg/g and 39.2 mg/g stigmasterol in EA, Convolvulus pluricaulis, CT and CD; 30.1 mg/g lupeol in CT.