J. AOAC Int. 95, 67-73 (2012). HPTLC fingerprint of four cycloartanes dikamaliartane-A (1) and dikamaliartane-B (2) and gummiferartane-1 (3) and gummiferartane-2 (4) in the gum resin from the leaf buds of Gardenia lucida or G. gummifera on silica gel with toluene – acetone 11:9. Detection by dipping in anisaldehyde reagent (anisaldehyde 0.5 % in methanol – acetic acid – sulfuric acid 17:2:1), followed by heating at 100 °C for 5 min. The hRf of compounds (1) to (4) were 60, 80, 90 and 70, respectively.

J. of China Pharm. 19 (22), 41-43 (2010). Yanreqing capsules are a herbal TCM preparation specially effective for heat clearing and detoxifying, and prescribed clinically to cure bronchitis, pneumonia, acute tonsillitis, urinary system infections and biliary tract infections. TLC of the extracts of the medicine on silica gel 1) for Baical Skullcap, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol and viewing in daylight; 2) for Cape jasmine, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 °C until the zones are visible.

J. Liq. Chromatogr. Relat. Technol. 35, 499-510 (2012). HPTLC of desvenlafaxine in dosage forms on silica gel with ethyl acetate - toluene - methanol 14:4:1 + 1 drop ammonia. Quantitative determination by absorbance measurement at 228 nm. The hRf value of desvenlafaxine was 48 and selectivity regarding matrix was given. Linearity was between 100 and 1000 ng/spot. The intermediate/inter-day/intra-day precision was below 0.2 % (n=6). The limits of detection and quantification were 10 and 100 ng/spot, respectively. Recovery (by standard addition) was between 97.9 and 99. 1%.

J. of China Pharm. 21 (3), 22-23 (2012). Rubingxiao tablet is a herbal TCM preparation prescribed to treat mastitis and hyperplasia of mammary glands. Quality control by qualitative identification of its major component drugs and quantitative determination of tetrahydropalmatine in it. TLC of the extracts of the preparations on silica gel 1) for Epimedium davidii Franch, with ethyl acetate – butanone – formic acid – water 10:1:1:1, detection at UV 366 nm, followed by spraying with 1 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Astragalus mongholicus, with chloroform – methanol 10:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were visible, evaluation at UV 366 nm; 3) for Poria cocos, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid - ethanol 1:4 and heating at 105 °C until the zones were visible, viewing under daylight.

Anhui Med. & Pharm. 16 (6), 768-769 (2012). Shiyiwei Huoxue tincture is a herbal TCM preparation for activation of blood circulation and is prescribed clinically to treat damages of soft tissues and diseases of the bone and joint system. For quality control, TLC of the extracts of the preparations on silica gel 1) for borneol, with n-hexane – ethyl acetate 17:3, detection by spraying with 1 % vanillin in sulfuric acid - ethanol 1:4 and heating at 105 °C until the zones are visible, evaluation in daylight; 2) for Rhizoma Corydalis, with n-hexane – chloroform – methanol 15:8:2, detection by exposure to iodine vapors and viewing in daylight and under UV 366 nm.

J. of China Pharm. 26 (2), 167-171 (2012). Niuhuangjingnao tablets are a herbal TCM effective specially in clearing heat, removing toxicity, relieving uneasiness of mind and body tranquilization, and are prescribed clinically to cure dizziness, swelling and pain in the throat. In order to perfect the procedure for the quality control of the medicine a method has been presented. TLC of the extracts of the medicine 1) for borneol, on silica gel with toluene – ethyl acetate 10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones were visualized, viewing in daylight; 2) for Rheum officinale, on silica gel with the upper phase of petroleum ether (60-90 ºC ) - ethyl acetate – formic acid 15:5:1, detection by viewing at UV 366 nm, followed by exposure to ammonia vapors and viewing at UV 366 nm; 3) for the rhizome of Chinese Goldthread, on silica gel with toluene – isopropanol - ethyl acetate – methanol – water 20:5:10:5:1, detection by exposure to ammonia vapors and viewing at UV 366 nm; 4) for Fructus Forsythiae, on silica gel with chloroform – methanol – glacial acetic acid 70:10:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were visualized, viewing in daylight; 5) for honeysuckle, on polyamide phase with ethyl acetate – methanol – formic acid 20:2:3, detection by viewing at UV 366 nm; 6) for the root of Kudzu vine and Cape jasmine, on silica gel with chloroform – methanol – water 28:10:1, detection by viewing at UV 366 nm, followed by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones were visualized, viewing in daylight.

Kinetic evaluation of the degradation process and identification of photoproducts by mass spectrometry. J. Liq. Chromatogr. Relat. Technol. 36, 2431-2445 (2013). HPTLC of difloxacin and its degradation products on silica gel with methylene chloride - methanol - 2-propanol - ammonia 25 % 4:4:5:2. Quantitative determination by absorbance measurement at 294 nm. The hRf values of difloxacin and its degradation products were 43, 25, 32, 39, respectively. Linearity was 1.2-2.4 µg/zone. LOD and LOQ were 0.01 and 0.03 µg/zone, respectively. Intermediate precision was below 2.0 %. Recovery (by standard addition) was in the range of 101.1-110.9 %.

Chinese J. of Northern Pharmacy 9 (4), 8-9 (2012). Shugan capsules are a herbal TCM herbal preparation for nourishing the liver and tonifying spleen, used for women’s climacteric symptoms. For quality control, TLC on silica gel 1) for Radix Paeoniae Alba, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 4:1 and heating mildly, viewing under daylight; 2) for Rhizoma Cyperi, with n-hexane - ethyl acetate 17:3, detection under UV 254 nm.