J. Planar Chromatogr. 28, 251-255 (2015). HPTLC of rivastigmine on silica gel with toluene - acetonitrile - formic acid 6:3:1. Quantitative determination by absorbance measurement at 540 nm. The hRF value for rivastigmine was 41. Linearity was in the range of 125-1250 ng/zone. LOD and LOQ were 22 and 66 ng/zone, respectively. The intermediate precision was below 8 % (n=3). Recovery ranged between 99 and 102 %.

J. Liq. Chromatogr. Relat. Technol. 38, 1767-1782 (2015). HPTLC of thiocolchicoside on silica gel with toluene - acetone - water 3:15:2. Quantitative determination by absorbance measurement at 277 nm. The hRf value of thiocolchicoside was 53. Linearity was in the range of 100-500 ng/zone. LOD and LOQ were 3 and 11 ng/zone, respectively. The intermediate/inter-day/intra-day precision (n=7) was below 1 %. Recovery (by standard addition) was in the range of 100-101 %.

J. Planar Chromatogr. 28, 395-397 (2015). HPTLC of 25-B-NBOMe (a N-(2-methoxy)benzyl-substituted phenylethylamine hallucinogen) in seized blotters (small, square pieces of absorbant paper impregnated with LSD or other hallucinogens) on silica gel with cyclohexane – toluene – diethylamine 15:3:2. Quantitative determination by absorbance measurement at 298 nm. The hRF value for 25-B-NBOMe was 34. Linearity was in the range of 19-115 μg/zone. LOD and LOQ were 7 and 22 μg/zone. The intermediate precision was below 6.3 % (n=6). Average recovery was 98 %.

by the combination of high-performance thin-layer chromatography with direct bioautography and mass spectrometry. J. of Chromatogr. A 1422, 310-317 (2015). Investigation of two tansy (Tanacetum vulgare L.) essential oils, obtained by steam distillation of the capitula with subsequent liquid-liquid extraction (oil 1) or with use of an auxiliary phase for the trapping of the steam components (oil 2), against Bacillus subtilis F1276, B. subtilis spizizenii (DSM 618), Xanthomonas euvesicatoria, Pseudomonas syringae pv. maculicola, Ralstonia solanacearum strain GMI1000 and Aliivibrio fischeri by using the coupling of HPTLC to direct bioautography (HPTLC-DB). Oil 2 was richer in components and provided more inhibition zones due to the advanced extraction process. Identification of the main bioactive components by scanning HPTLC-direct analysis in real time mass spectrometry (HPTLC-DART-MS) and solid-phase microextraction GC electron impact MS (SPME-GC-EI-MS) as cis- and trans-chrysanthenol as well as trans-chrysanthenyl acetate. The results indicated that cis-chrysanthenol exhibited antibacterial effects against all tested bacteria. Trans-chrysanthenol inhibited B. subtilis, R. solanacearum and A. fischeri, and trans-chrysanthenyl acetate was an inhibitor for X. euvesicatoria, R. solanacearum and A. fischeri. However, the ionization characteristics and the recorded mass spectra showed that DART is a softer ionization technique than EI and more affected by ambient conditions and thus prone to additional oxidation products, although HPTLC-DART-MS resulted in a comparable fragmentation.

J. Liq. Chromatogr. Relat. Technol. 39, 264-270 (2016). HPTLC of estradiol hemihydrate on silica gel (1) or RP-18 (2) with dichloromethane – ethyl acetate 4:1 for (1) and methanol – water 9:1 for (2). Quantitative determination by absorbance measurement at 200 nm. The LOD was 239 ng/zone using (1) and 93 ng/zone using (2).

J. Planar Chromatogr. 29, 227-228 (2016). HPTLC of carbaryl in visceral sample on silica gel with hexane – ethyl acetate 9:1. Detection by spraying with 10 % NaOH solution followed by a mixture of a 10 % aqueous sodium bromide solution and a 10 % aqueous copper chloride solution (mixture ratio most likely 1:1, however, not reported). The hRF value for carbaryl was 51.

Chinese J. Mod. Drug Appl. 8 (5), 3-4 (2014). Danshen Wuweizi Pian tablet is a herbal TCM preparation for the treatment of coronary heart disease, acute and chronic persistent hepatitis and neurasthenia. For quality control, TLC (1) for Salvia miltiorrhiza Bge. and the standard tanshinone IIA, on silica gel with toluene – ethyl acetate 19:1, detection in daylight; (2) for Schisandra chinensis (Turcz.) Baill., on silica gel with petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm.

J. Ethnopharmacol. 193, 627-636 (2016). HPTLC of hyperoside (1), rutoside (2), apigenin (3), spiraeoside (4), quercetin (5), and isoquercitrin (6) on silica gel with ethyl acetate – formic acid – glacial acetic acid – water 100:11:11:26. Detection by spraying with natural product/PEG reagent, followed by drying for 10 min. Quantitative determination by absorbance measurement at 366 nm. The hRF values for (1) to (6) were 55, 37, 95, 6, 92 and 57, respectively._x000D_