J. Planar Chromatogr. 24, 232-235 (2011). HPTLC of darunavir ethanolate in tablets on silica gel, prewashed with methanol, with toluene - ethyl acetate - methanol 7:2:1 in a twin-trough chamber lined with filter paper and saturated with mobile phase for 30 min at room temperature (25 +/- 2 °C). Quantitative determination by densitometry in absorbance mode at 267 nm. The hRf of darunavir ethanolate was 47. Linearity was between 250 and 1750 ng/band with r = 0.9994. The limits of detection and quantification were 15 and 46 ng/band, respectively. The intra-day and inter-day precision was (%RSD, n = 6) between 0.5-0.9 % and 1.1-1.3 %. Recovery (n = 6) was 99.3-101.2 %.

J. Planar Chromatogr. 24, 264-267 (2011). TLC of crebanine on silica gel with toluene - ethyl acetate - methanol 14:3:3 in a twin-trough chamber saturated for 1 h at 25 +/- 2 °C. Detection under UV light at 254 nm. Quantitative determination by densitometry in absorbance mode at 286 nm. The method precision (%RSD, n = 3) and the instrument precision (%RSD, n = 6) were 0.9 and 0.5 %, respectively. The repeatability (%RSD, n = 5) was 0.8; the accuracy as average recovery was 100.1 %. The limit of detection and quantification was 10 and 15 ng/zone, respectively. Linearity was between 100-500 ng/zone. The hRf value was 54.

J. Planar Chromatogr. 23, 315-319 (2010). HPTLC of polyphenols (extracted from Quercus robur) and quercetin, chlorogenic acid, gallic acid, and rutin as standards on silica gel with ethyl acetate - water - formic acid 87:3:10 in an unsaturated twin-trough chamber. Detection by spraying with 1 % 2-aminoethyl diphenylborinate (natural products reagent) followed by 2 % polyethylene glycol 4000 solution as well as by spraying with 2 % phosphomolybdic acid reagent followed by heating at 120 °C for 5 min. Evaluation by densitometry in absorption mode at 700 nm.

Der Pharmacia Letter 2(4), 440-446 (2010). TLC of glibenclamide on silica gel with toluene - ethyl acetate - methanol 16:1:2. The hRf value was 45. Quantitative determination by densitometry in absorbance mode at 229 nm. The method was linear in the range of 40-200 ng/band. The recovery was 99.8 %.

J. Planar Chromatogr. 24, 534-538 (2011). HPTLC of clarithromycin in plasma on silica gel (prewashed with methanol) with ethyl acetate - methanol - 15 % ammonium acetate (pH 10.6) 7:2:1 in a twin-trough chamber with saturation for 15 min. Detection by dipping into xanthydrol solution (10 % in methanol). Quantitative determination by densitometry in absorbance mode at 506 nm. The hRf was 62. The method was linear over the range of 0.1-3.0 µg/mL (r2 = 0.9974). The recovery (by standard addition) was over 85 %. The intra-day and inter-day precision (%RSD) of the assay was in the range of 0.8-4.6 %. The recovery was above 95 %.

J. Planar Chromatogr. 24, 130-135 (2011). TLC of Juniperus essential oils (containing terpenoids, monoterpenes, sesquiterpenes and alcohols) with guaiazulene and cineole as standards on silica gel with ethyl acetate - toluene 1:19. Detection by spraying with anisaldehyde reagent followed by heating for 5 min at 100 °C and examination under daylight. The hRf values were 42 and 89 for cineole and guaiazulene, respectively.

J. Liq. Chromatogr. Relat. Technol. 34, 1783-1799 (2011). HPTLC of isotretinoin in topical formulations on silica gel with toluene - methanol 9:1. Quantitative determination by absorbance measurement at 340 nm. The hRf of glycyrrhizin was 43. Linearity was 100-500 ng/zone. LOD and LOQ were found to be 13 and 42 ng/zone. The intra-day and inter-day precision precisions (n = 5) were 1.8 % and 1.6 %, respectively. Recovery (by standard addition) ranged from 99.1-100.6 %.

J. Liq. Chromatogr. Relat. Technol. 32, 2437-2450 (2009). HPTLC calliterpenone (1) and calliterpenone monoacetate (2) in the leaves of Callicarpa macrophylla on silica gel with methanol - water 9:11. Quantitative determination by absorbance measurement at 210 nm. The hRf values of (1) and (2) were 43 and 73, respectively. Linearity was between 1-5 µg/zone for (1) and (2). LOD and LOQ were 230 and 780 ng/zone for (1) and 220 and 730 ng/zone for (2). Intra-day and inter-day precisions ranged between 1.3-1.7 % for (1) and 1.1-1.7 % for (2). Recoveries (by standard addition) were between 97.5-100.8 % for both.