J. Planar Chromatogr. 25, 290-294 (2012). HPTLC of withaferine A (1), 1,2 deoxy-withastramonolide (2), withanolide A (3), and withanolide B (4) in Withania somnifera on silica gel with dichloromethane - toluene - methanol - acetone - diethyl ether 15:15:6:2:2. Quantitative determination by absorbance measurement at 235 nm. The hRf values of (1) to (4) were found to be 58, 61, 68 and 79, respectively. Linearity was in the range of 200-1200 ng/band. Average recoveries were 98, 99.5, 98 and 99 % for compounds (1) to (4), respectively.

J. of Qilu Med. & Pharm. 31 (2), 75-78 (2012). Pudilan Xiaojan oral liquid is a herbal TCM preparation effective in clearing heat and removing toxicity, because of its antiinflammatory activity it is used to cure swelling caused by furuncles, pharyngitis, lymphadenitis and tonsillitis. Dandelion is the key component drug of the preparation. Analysis of the components of dandelion in the preparation by TLC on silica gel with chloroform – methanol – formic acid 18:2:1, detection under UV 366 nm.

by thin-layer chromatography) (Chinese) Chinese J. Ethnomed. Ethnopharm.(3), 53-54 (2012) Melodinus fusiformis is a herbal TCM drug specially effective for curing rheumatism. TLC of the extractsand standard oleanolic acid of the crude drug on silica gel with n-hexane – ethyl acetate 7:3, detection by spraying with 5 % phosphomolybdate in n-propanol and heating using a air blower until the zones are visible.

J. Planar Chromatogr. 25, 30-35 (2012). HPTLC of rutin (1), narcissin (2), nicotiflorin (3) and isoquercitrin (4) on silica gel with ethyl acetate - 1,2-dichloroethane - acetic acid - 85 % formic acid - water 100:25:10:10:8. Quantitative determination by absorbance measurement at 360 and 387 nm. Intraday and interday precisions were in the range of 1.5-1.9 % and 1.6-1.9 %, respectively. Recovery was found to be 98.2-101.4 % for (1), 98.0-101.1 % for (2), 98.2-100.5 % for (3) and 98.1-101.9 % for (4).

J. Planar Chromatogr. 25, 65-71 (2012). HPTLC of dapsone in presence of its oxidative degradants on silica gel with acetate - toluene 1:1. Quantitative determination by absorbance measurement at 289 nm. Linearity was in the range of 0.5-6.0 µg/zone. Recovery was 99.4 %. The method showed comparable results to a validated HPLC method.

Inner Mongolian J. of Trad. Chinese Med. 10, 35-36 (2012). Jiangding Doukou Qufeng compound oral liquid is a herbal TCM preparation prescribed to effectively cure flatulence, loss of appetite, nausea, and vomiting. TLC of the extracts of the preparations on silica gel 1) for Cortex Cinnamomi and Foeniculum vulgare, with petroleum ether (60-90 ºC) – ethyl acetate 17:3, detection by spraying with 2,4-dinitrophenylhydrazine solution in 2N hydrochloric acid and viewing in daylight; 2) for Rhizoma Zingiberis, with petroleum ether (60-90 ºC) – chloroform – ethyl acetate 2:1:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C until the zones were visible in daylight.

Chinese J. Mod. Drug Appl. 6 (12), 131-132 (2012). Shisiwei Dangui Huoxue mixture is a herbal TCM for promoting blood circulation and relieving pain, and is prescribed clinically to treat blood stasis, local swelling caused by lesion of main and collateral channels of the injured limb after catagma. For quality control, TLC on silica gel 1) for Angelica sinensis, with n-hexane – ethyl acetate 9:1, detection at UV 366 nm; 2) for Radix Paeoniae Alba, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 followed by mild heating and evaluation in daylight.

J. Sep. Sci. 36, 2373-2378 (2013). HPTLC of 6alpha,7alpha-diacetoxy-13-hydroxy-8(9),14-labdadien (1), 13-hydroxy-5(10),14-halimadien-6-one (2), and 9-hydroxy-13(14)-labden-16,15-olide (3) in the leaves of Vitex trifolia L. on silica gel with chloroform - acetone 49:1. Detection by spraying with vanillin-sulfuric acid reagent. Quantitative determination by absorbance measurement at 610 nm. The hRf of compounds (1), (2) and (3) were 74, 57 and 51, respectively. Linearity was 333-1000 ng/zone for (1) and (2) and 670-2000 ng/zone for (3). LOD were in the range of 73, 56, and 92 ng/zone and LOQ were found to be 242, 186, and 307 ng/zone for (1), (2) and (3), respectively. The intra- and inter-assay precisions were in the range of 1.0-1.2 % and 1.1-1.3 %, respectively for compounds (1) to (3). Recovery (by standard addition) ranged 99.1-102.7 %.