J. Ethnopharmacol. 134, 992-995 (2011). HPTLC of fumaric acid and fumaric acid conjugates (as dimethyl fumarate) in the aerial parts of Fumaria indica on silica gel with formic acid – chloroform – butanol – heptane 3:4:8:11. Quantitative determination by absorbance measurement at 260 nm.

62nd Indian Pharmaceutical Congress Abstract No. F-244 (2010). TLC of fluvoxamine maleate on silica gel with benzene – methanol 5:4. The hRf value was 52. Quantitative determination by absorbance measurement at 256 nm. The linearity was in the range of 500-3000 ng/band with r2=0.998. The drug was subjected to acidic, alkaline and oxidative, dry heat, UV and photolytic stress. Since the method could effectively separate the drug from its degradation products, it can be used for stability studies.

62nd Indian Pharmaceutical Congress Abstract No. F-242 (2010). TLC of gatifloxacin and ornidazole on silica gel with n-butanol – ethanol – 8M ammonia 10:1:3. Quantitative determination by absorbance measurement at 299 nm. The hRf value of gatifloxacin was 27 and of ornidazole 83. The method was found to be linear between 20-100 ng/zone for gatifloxacin and 50-250 ng/zone for ornidazole (r2> 0.99). The limit of detection and quantitation were found to be 4.1 and 12.5 ng/zone, respectively for gatifloxacin and 10.3 and 31.2 ng/zone, respectively for ornidazole.

62nd Indian Pharmaceutical Congress Abstract No. F-251 (2010). TLC of levocetirizine and phenylpropanolamine on silica gel with methanol – ethyl acetate – toluene – ammonia 15:4:5:2. Quantitative determination by absorbance measurement at 210 nm. The hRf value was 30 and 60 for levocetrizine and phenylpropanolamine, respectively. The linearity was in the range of 45-270 ng/band for both drugs.

Acta Chromatographica 20(4), 625-636 (2008). TLC of of aceclofenac, paracetamol, and chlorzoxazone on silica gel (prewashed with methanol) with toluene – 2-propanol – ammonia 10:10:1. Detection and quantification by densitometry at 274 nm. The hRf values of aceclofenac, paracetamol, and chlorzoxazone were 28, 72 , and 51, respectively. The linearity was in the range of 400–1400 ng/band for aceclofenac, 2–7 µg/band for paracetamol, and 1–3.5 µg/band for chlorzoxazone, with r=0.9995, 0.9993, and 0.9996, respectively. The recovery of aceclofenac was 99.5-100.4 %, for paracetamol 100.0-100.5 %, and for chlorzoxazone 99.4-99.8 %.

CBS 105, 13-15 (2010). HPTLC of PVC foil samples on silica gel with isooctane – toluene – diethyl ether – ethyl acetate 8:7:4:1 after chamber saturation for 10 min, up to a migration distance of 65 mm. Detection of the biological activity of any compound migrated from the plastic foils in migration studies by dipping in Vibrio fischeri bacteria suspension and documentation with the Bioluminizer. Also direct extraction of additives from plastic foils by the TLC-MS Interface coupled to an Agilent LC-MS system and recording of the eluted additives in the positive ESI mode. Exact masses of unknowns were calculated with MassWorks software allowing their improved specification and thus their confirmation.

J. AOAC Int. 93, 804-810 (2010). HPTLC of rutin on amino phase with ethyl acetate - formic acid - methanol - water 100:9:11:17 at room temperature. Detection by spraying with natural products reagent. Linearity was between 0.95 and 4.78 µg/zone. LOD and LOQ were 330 and 630 ng/zone, respectively. The %RSD for six replicates at three concentration levels was less than 3 %, while the recovery was between 97.3-103.3 %. For the three-dimensional image analysis of chromatograms images were taken from each plate using a HP flatbed scanner at an optical resolution of 300 dpi in normal mode. The pictures were stored in TIFF file format. Evaluation was performed using Melanie 7.0 software. Following automatic detection of the zones the program computed the 3D image of a selected region from the HPTLC plate. The volume of the resulting cone-shaped zone was used as numerical data to construct the calibration curve.

J. Planar Chromatogr. 24, 136-139 (2011). HPTLC of ethanolic extracts of plant material and betaine on silica gel, prewashed with methanol, with methanol - water 9:1 in a twin-trough chamber saturated for 30 min. Detection under UV light at 254 and 366 nm and by spraying with Dragendorff’s reagent or with sulfuric acid reagent followed by heating at 120 °C for 10 min (Dragendorff’s reagent) or at 110 °C until the intensity of the fluorescent zones reached a maximum (sulfuric acid). Quantitative determination by densitometry at 550 nm. The calibration range was between 4 and 30 µg/band. LOD and LOQ was 1 and 4 µg/band, respectively. The repeatability (n = 6) was 1.7 %. The recovery was between 99.1-101.9 %. The inter-day and intra-day precision (n = 3) was between 1.1- 2.2 % and 1.2-2.2 %, respectively.