Isolation of the enantiomers. Chromatographia 68 (11-12), 1048-1051 (2008). TLC resolution of the enantiomers of the racemic drugs ketamine and lisinopril on silica gel with 1) ethyl acetate - methanol - water 3:1:1 and enantiomerically pure tartaric acid and (-)-mandelic acid as chiral impregnating reagents, for ketamine; and 2) acetonitrile - methanol - water - dichloromethane 14:2:2:1 with (+)-tartaric acid as the impregnating agent for lisinopril and using (+)-tartaric acid as mobile phase additive; and 3) acetonitrile - methanol - (+)-tartaric acid (0.5 % in water, pH 5) - acetic acid 70:10:11:7 which enabled successful resolution of the enantiomers of lisinopril. Investigation of the effects of temperature, pH, and the amount of chiral selector. Detection with iodine vapour. Isolation and identification of the separated enantiomers. The LOD was 0.25 and 0.27 µg for each enantiomer of ketamine with (+)-tartaric acid and (-)-mandelic acid, respectively, whereas 0.14 and 0.16 µg for each enantiomer of lisinopril with (+)-tartaric acid (both conditions) and (-)-mandelic acid, respectively.

J. AOAC Int. 93, 771-777 (2010). HPTLC of the diastereomer A and B of cefuroxime axetil on cellulose (activated by heating at 60 °C) with 1 % aqueous beta-cyclodextrin - methanol 15:1. Quantitative determination by absorbance measurement at 285 nm. The hRf of diastereomer A was 87 and for diastereomer B 93. Linearity was between 100-450 ng/zone for both diastereomers. Precision was good with %RSD values below 2.67 %. The recovery was in the range of 96.6-104.2 %. The LOD for both diastereomers was 40 ng/zone.

J. Planar Chromatogr. 30, 350-356 (2017). HPTLC for the enantioresolution of atenolol, betaxolol, and orciprenaline racemic mixtures using the chiral selector (S)-Glu as an additive of the stationary phase (A) or the mobile phase (B). For method (A), the chiral selector (50 mL of 0.5 % (S)-Glu) was mixed with a slurry of silica gel (25 g) and the plate was developed with acetonitrile – methanol – dichloromethane – water 10:3:3:2. For method (B), the mobile phase was acetonitrile – methanol – water 7:1:1 admixed with 0.5 % (S)-Glu. Detection by exposure to iodine vapor. LOD for each enantiomer was in the range of 1.4-1.9 μg/zone. Recovery was between 96.2-99.1 % for_x000D_
both (S)- and (R)-atenolol.

J. Chromatogr. 503, 226-229 (1990). Ligand-exchange TLC on chiral plates with acetonitrile – methanol – water in different proportions. Detection by spraying with 0.2 % ninhydrin in acetone and heating at 100 °C for 10 min. The method is amenable to quantification by densitometry.

J. Planar Chromatogr. 4, 338-340 (1991). TLC of optical isomers and 22 nitro substituted aromatic hydrocarbons on RP-18 silica with aqueous solutions containing per 100 mL sodium chloride (2.5 g), urea (5.0 g), methanol (30,0 mL), and 0.1 M a-cyclodextrin resp. containing sodium chloride (2.5 g), urea (26.0 g), methanol (30 mL, and 0.1 M ß-cyclodextrin. Detection under UV.

J. Planar Chromatogr. 7, 103-107 (1994). TLC of charged and uncharged racemic solutes (e.g. warfarin and its p-chloro derivative, 2,4-DNP-DL-citrullin, 2,4-DNP-DL-ethionine, DL-4-amino-N-methylpteroylglutamic acid racemates) on RP-18 silica by eluting with solutions of different pH containing 2% 2-propanol and high concentrations of bovine serum albumin. For several solutes there was a linear relationship between Rf/(1 -Rf) and albumin concentration.

Anal. Letters 28, 2041-2048 (1996). TLC on silica with acetonitrile - water 2:5 containing 2-o-[(R)-2-hydroxypropyl]-b-CD as additive. Detection by spraying with a solution of 1.5 g salicylaldehyde in 100 mL toluene and heating at 50 °C for 10 min.

J. Liq. Chrom. & Rel. Technol. 22, 41-50 (1999). Separation of selected amino acids into their enantiomers by TLC on a chiral phase. A new valence optical topological index and valence optical Gutman index are proposed which enable distinction between isomers of L and D configuration. HPTLC of optical isomers of D and L amino acids (alanine, norvaline, norleucine, glutamic acid, phenylalanine, tyrosine, tryptophan, proline) on Chir HPTLC plates with methanol - water - acetonitrile 1:1:4. Visualization by dipping into a solution of 0.3% ninhydrin in acetone and heating at 110°C for 10 min.