Chiral separations with aqueous solvents and liquid-liquid systems. J. Chromatogr. 635, 346-348 (1993). Examination of the enantiomers of substituted tryptophans by TLC on micro crystalline cellulose with aqueous solvents and liquid-liquid systems. Discussion of the mechanism of separation in liquid-liquid systems.

J. Liquid Chromatogr. 17, 1125-1139 (1994). TLC on silica with 1) petrol ether (40-60 °C) - ether - formic acid 50:50:1, 2) water - acetic acid 9:1. Visualization under UV 254 and 366 nm before and after spraying with an ethanolic solution of KOH (5%) without and with heating at 100 °C for 10 min. Also HPLC method.

Biomed Chromatogr. 11, 286-288 (1997). TLC on silica gel impregnated with (1R,3R, 5R)-2-azabicyclo[3,3,0]octan-3-carboxylic acid with 0.5 M NaCl - acetonitrile in different ratios and addition of methanol in some cases. Visualization under UV 254 nm for dansyl-DL-amino acids. Development with different combinations of acetonitrile - methanol - water and detection by spraying with 0.2% ninhydrin in acetone for amino acids.

J. Chromatogr. B 752 (2), 311-322 (2001). The peptide mix obtained from in-gel or on-blot degestion wax analysed directly after degestion or after concentration on POROS R2 beads. 2 DE of Mycobacterium bovis BCG Chicago cell proteins. Eight protein spots were identified using four preparation procedures for MALDI-MS. Overall, on-blot degestion was as effective as in-gel degestion. Whereas higher signal intensity resulted after concentration, hydrophilic peptides are better detected by direct measurement of the peptide mix without POROS R2 concentration.

J. Chromatogr. Sci. 43 (10), 542-548 (2005). The usefulness of TLC as an efficient measuring technique in the studies of oscillatory trans-enantiomerization of profens from the S to the R configuration (and vice versa) during their storage as 70 % ethanol solutions is demonstrated in the literature. S-(+)-ibuprofen, S-(+)-naproxen, and S,R-(±)-2-phenylpropionic acid are utilized as the test profens. It is proven possible to show oscillatory instability with the racemic S,R-(±)-2-phenylpropionic acid also. Correctness of the TLC assessment is successfully confirmed by means of polarimetry. Upon these preliminary results, it is concluded that the most probable mechanism might embrace the keto-enol tautomerism because of a convenient migration of the proton from one moiety of the profen molecule to another in an aqueous medium. To indirectly verify this hypothesis, profens are stored in dichloromethane, deliberately hampering their ability to dissociate and to re-structure. It is shown that the non-aqueous solvent considerably suppresses, although they do not completely eradicate, the oscillatory trans-enantiomerization of profens. In view of these findings, the reports which claim a predominant therapeutic potential of the respective S-profens become less convincing and certainly need reconsideration.

59th Indian Pharmaceutical congress F-211, 440, (2007). TLC of isomers of carvedilol on chiral silica gel (prepared with beta-cyclodextrin) with methanol - water 5:1. Evaluation at 366 nm. The R(+) and the S(-) isomer of carvedilol had hRf values of 89 and 81, respectively. The linearity range was 50 - 500 µg/zone. Limit of detection and quantification was 10, 12 and 40, 42 µg/zone respectively for R(+) and S(-) isomers. The preparation of R(+) and S(-) isomers of carvedilol was found to be in the ratio 3:2 in bulk and formulations.

J. Chromatogr. A 1217(8), 1395-1398 (2010). TLC of atenolol, propranolol and salbutamol and their enantiomers by using different modes of loading/impregnating the Cu(II) complexes of L-proline, L-phenylalanine, L-histidine, N,N-dimethyl-L-phenylalanine, and L-tryptophan on silica gel with 1) the Cu(II)-L-amino acid complex as chiral mobile phase additive, 2) ascending development of plain commercial plates in the solutions of Cu complex, and 3) using a solution of Cu(II)acetate as mobile phase additive for the commercial TLC plates impregnated by development with the amino acid solutions. Detection by exposure to iodine vapor. The detection limit was 0.18 µg for each enantiomer. For the best method performance the Cu(II)cation has to be involved.

J. Planar Chromatogr. 29, 176-183 (2016). HPTLC of atracurium besylate (1) and atropine sulfate (2) on silica gel with acetonitrile – methanol – dichloromethane – glacial acetic acid – water containing 70 mg L-(+)-tartaric acid 70:10:5:7:1, pH 5 for (1), and methanol – water containing 40 mg L-histidine and 20 mg copper(II) acetate 22:3, pH 7 for (2). Quantitative determination by absorbance measurement at 280 nm for (1) and 215 nm for (2). The hRF values for (1) and (2) were 51 and 65, respectively. Linearity was in the range of 2-14 μg/zone for (1) and 5-35 μg/zone for (2). Intermediate precisions were below 1 %. The LOD and LOQ were 0.5 and 1.6 µg/zone for (1) and 1.2 and 3.6 µg/zone for (2). Average recovery was found to be 103.4 % for (1) and 96.6 % for (2).