Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany; stuetz.l@lw-online.de)

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      129 068
      Thai mango and pineapple puree and juice analyzed by high-performance thin-layer chromatography hyphenated with effect-directed assays
      Gertrud E. MORLOCK*, N. WUTTHINITHISANAND, D. RAUHUT
      (*Institute of Nutritional Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      Molecules, 26 (24), 7683 (2021). Samples were ultrasound-assisted extracts of fruit puree and juice (pre-treated with sulfur dioxide or ascorbic acid) of Ananas comosus (Bromeliaceae) and Mangifera indica (Anacardiaceae). HPTLC on silica gel with toluene – ethyl acetate – methanol – formic acid 120:90:35:3. Detection under white light, UV 254 nm and 366 nm, before and after  derivatization by immersion (2 s, 3 cm/s) into anisaldehyde sulfuric acid reagent and  diphenylamine aniline reagent, followed by heating at 110 °C for 5 min. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for enzymatic inhibition (acetyl-cholinesterase, tyrosinase); C) for activity against Gram-negative (Aliivibrio fischeri bioluminescence assay) or Gram-positive bacteria (Bacillus subtilis bioassay). Active compounds were far more present in puree than in juice extracts, and differences were also seen between cultivars. Ascorbic acid (hRF 37), used as additive for the mango puree, was active as antioxidant and as transiently disruptive for A. fischeri metabolism and bioluminescence.

      Classification: 27, 32e, 35b
      107 154
      Analysis of biodiesel conversion using thin-layer chromatography and nonlinear calibration curves
      S.N. FEDOSOV*, J. BRASK, X. XU (*Dept. Molecular Biology, Aarhus Univ., Science Park, Gustav Wieds Vej 10C, 8000 Aarhus C, Denmark)

      J. Chromatogr. A 1218 (19), 2785-2792 (2011). Examination of the applicability of TLC for the analysis of biodiesel conversion. Biodiesel is a complex mixture which complicates the analytical separation and requires a large set of data for understanding reaction kinetics. A flame ionization detector (FID) and a modified TLC staining procedure were evaluated in comparison with the well-established but time-consuming and expensive GC and HPLC methods. The TLC staining method is suited for quantitative analysis due to no background. Demonstration by using several experimental samples produced by enzymatic conversion of rapeseed oil to biodiesel. It was found that the first reaction step (6 h) resulted in 85–95 % conversion and the second step (after removal of glycerol and water) increased the yield to 97–98 %. All components of the mixtures were separated and quantified. Relation of the biodiesel contents measured by TLC and GC gave the values of 1.03 ± 0.07 (TLC-staining) and 0.95 ± 0.04 (TLC–FID), which indicated the applicability of the TLC methods.

      Classification: 35
      78 102
      Separation of optical brighteners for plastics and migration into water and olive oil
      B. ILIANO, A.M. OUDAR, J. GOSSELE, (Ministry of Public Health, Inst. of Hyg. and Epidemiology, Dept. of Pharmaco-Bromatology, Brussels, Belgium)

      Deut. Lebensm. Rundschau 91, 205-208 (1995). HPTLC of optical brighteners (Leucopur, Azur 4, Azur 5, Hostalux ABC, Uvitex, Eastobrite) on RP-18 with acetonitrile - dichloromethane 19:1, acetonitrile - water 19:1 and methanol - water 97:3. The organic mixture showed better separation than the aqueous phases. Detection under UV.

      Keywords:
      Classification: 30, 35
      112 136
      Thin-layer chromatographic monitoring of sonolytic degradation of surfactants in wastewaters
      I. REZIC (Laboratory of Analytical Chemistry, Department of Applied Chemistry, Faculty of Textile Technology, University of Zagreb, Croatia, iva_rezic@net.hr)

      J. Planar Chromatogr. 26, 96-101 (2013). HPTLC of cetylpyridinium chloride (1), sodium dodecyl sulfate (2) and Triton X-100 (3) on silica gel with methanol - water 1:1. Quantitative determination by absorbance measurement at 254 nm. The hRf values for (1) to (3) were 6, 74 and 91, respectively. Linearity was in the range of 170-1740 µg/zone for (1), 250-1980 µg/zone for (2) and 160-1640 µg/zone for (3). LOQ was 75 ng/zone for (1), 90 ng/zone (2) and 65 ng/zone for (3).

      Classification: 35a
      61 257
      (Quantitative analysis of sucrose fatty acid esters by thin-layer chromatography
      Q. DENG (Deng Qinying), K. LI (Li Kaozheng), X. SUN (Sun Xiaowei), (Dep. Chem., Zhongshan Univ., Guangzhou, P.R. China)

      J. China Assoc. Inst. Anal. 6, 19-22 (1987) (Fenxi Ceshi Tongbao). TLC on silica with chloroform - methanol - acetic acid - water 80:10:8:2. Detection by exposing to iodine vapour. Quantification by densitometry at 520 nm.

      Classification: 11a, 35a
      73 169
      Separation of different types of surfactants by thin-layer chromatography
      A. KRUSE, N. BUSCHMANN*, K. CAMMANN, (Dept. of Anal. Chem., Westfäl. Wilhelms Univ., Wilhelm-Klemm-Str. 8, D-48149 Münster, Germany)

      J. Planar Chromatogr. 7, 22-24 (1994). TLC of surfactants (anionic, cationic, non-ionic, amphoteric) by four-fold development on silica with 1. methanol - 2 N NH3 3:1, 2. THF - acetone 1:9, 3. chloroform - methanol 9:1, 4. chloroform - methanol - 0.1 N sulfuric acid 80:19:1. Visualization by dipping in Dragendorff's reagent. The reliability of the method has been tested for 16 surfactants, for which the Rfs are reported.

      Classification: 35a
      106 179
      Chromatographic behavior of food additives on thin layers of titanium(IV) silicate ion-exchanger
      V. GHOULIPOUR, S. AMINI, A. HAGHSHENAS, S. WAQIF-HUSAIN* (*Chemistry Department, Faculty of Science, Science and Research Branch, Islamic Azad University, P. O. Box 14515-775, Poonak-Hesarak, Teheran, Iran; syedwaqifhusain@yahoo.com)

      J. Planar Chromatogr. 23, 250-254 (2010). TLC of 30 food additives (aldehydes, organic acids, esters and alcohols, and various sodium salts) on titanium(IV)silicate ion-exchanger with 10 different mobile phases: e. g. methanol, heptane - diethylether 4:1, aqueous ammonia - methanol - ethyl acetate 1:3:6, methanol - aqueous ammonia 9:1, methanol - ethanol 17:3, 0.5 M ammonium sulfate solution, 0.25 M, 0.5 M and 0.1 M potassium bromide solution, and phosphate buffer pH 2.5 in a twin-trough chamber without chamber saturation. Detection by spraying with 1 % iron chloride solution, 0.5 % potassium permanganate and 3 % barium chloride solution 1:1, 1 % ninhydrin in ethanol, 2 % phosphomolybdic acid in ethanol, and 5 % potassium dichromate in concentrated sulfuric acid. The study shows that the quality of separation depends to a large extent on the mobile phase, and selectivity is achieved by varying the composition of the mobile phase.

      Classification: 35b