J .Chromatogr. Sci., 29, 423-427 (1991). HPTLC of sulfonamide on silica developed three times with mixtures of methanol, containing 2% NH3 (25%) and dichloromethane 3:7, 14:85 and 5:95. Detection by fluorescence quenching, or by spraying with a fluorescamine solution, and scanning by fluorescence at 366/>400 nm. Detection limit 0.32 ng. Limit of quantification, 4 µg/kg for milk and meat samples.

Acta Pharma. Sinica (Yaoxue Xuebao) 28, 532-536 (1993). TLC of berberine on silica with ethyl acetate - methyl acetate - methanol - water 27:23:6:5. Quantification by densitometry at 350 nm.

Hanzaigaku Zasshi (Acta Criminol. Med. Legalls Jap.) 60, 149-154 (1994). Simultaneous TLC detection of spermine and choline from human semen, and semen of some other animals, and of a number of fruit and vegetable juices. Discussion of the distinction between the chromatograms for human semen and those for other samples.

Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 16, 418-419 (1996). TLC on silica with chloroform - benzene - acetone - methanol 5:5:3:1. Detection under UV 254. Quantification by densitometry at 300, 200 nm, respectively. Detection limits 60, 60, 400 ng, respectively. Precision between 1.78 and 3.21% (within plate) and between 2.75 and 4.05% (inter plate).

Chromatographia 47, 695-700 (1998). TLC on silica gel with chloroform - methanol 9:1. Detection by dipping into isonicotinic acid hydrazide (IAH) reagent for 20 s, and then into chloroform - liquid paraffin 9:1. Quantitation by fluorodensitometry at 366/>460 nm. Detection limit 1 ng.

The transformation of icariin by intestinal flora.) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 31 (11), 834-837 (2000). TLC on silica gel with petroleum ether (30-60°C) - acetone - formic acid 35:15:1. Detection by spraying with 2 % AlCl3 in ethanol, and under UV 254 nm. Identification of the metabolites, icaritin and baohuoside I, by ESI/MS

J. Planar Chromatogr. 16, 374-376 (2003). HPTLC of mannose and lactulose from urine on amino-modified silica gel with acetonitrile - water 7:3. After drying the plates were heated at 170°C for 20 min. Determination of lactulose by densitometry at 366 nm. Visualization after dipping into paraffin - hexane 1:2, and drying for 5 min at 100°C. The plate was immersed in three detection reagents: 1 L 0.1 mol/L AgNO3 and 200 mL acetone for 2 s, drying in air; 4 g NaOH in 4 mL water and 200 mL methanol for 2 s, drying for 2 min at 100°C; 20 g sodium thiosulfate dissolved in 100 mL water and 100 mL ethanol for 6 s, drying for 2 min at 100°C. Determination of mannitol by densitometry in absorbance mode at 660 nm.

Braz. J. Pharm. Sci. 49, 845-851 (2013). HPTLC of metoprolol (1) and hydrochlorothiazide (2) in human plasma on silica gel with chloroform - methanol - ammonia 18:2:1. Quantitative determination by absorbance measurement at 239 nm. The hRF values for (1) and (2) were 48 and 13. Linearity was in the range of 20-120 μg/zone for (1) and 2-12 μg/zone for (2). The intermediate/interday/intra-day precisions were below 5 % (n=3). The LOQs were 20 and 2 μg/zone for (1) and (2), respectively. Recoveries were between 70.6 and 84.9 %.