IPC 56th 2004, Abstract No. G-20. Simultaneous HPTLC determination of loratadine and ambroxol in combined dosage form on silica gel with n-hexane - dichloromethane - triethanolamine 11:8:1.The Rf value of loratadine and ambroxal was found to be 0.40 and 0.16 respectively. Quantitative evaluation by scanning at 254 nm.The method was linear in the range of 0.2 -1 mg/spot for loratadine and 1.2 - 6 mg/spot for ambroxol with recovery of 98.2 - 98.5 %. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ.

56th IPC 2004, Abstract No. GP-47. Stability indicating HPTLC determination of oxcarbazepine in tablet dosage form on silica gel with toluene - methanol 4:1. The Rf value of oxcarbazepine was 0.17. Quantitative determination by scanning at 255 nm. The compound was subjected to acid and alkali hydrolysis, oxidation, dry heat, and photo degradation. All degraded products were well resolved from the pure drug. The method was validated for accuracy, precision, linearity, robustness, and recovery.

J. Pharm. Biomed. Anal. 39, 581-586 (2005). For post-production quality control of camptothecin derivatives irinotecan (CPT 11) and topotecan (TPT), HPLC and HPTLC methods have been developed which were suitable for identification, determination of purity and quantification. HPTLC on silica gel with methylene chloride - methanol - formic acid - water 82:24:2:1. After development, the plate was soaked in 15 % paraffin in n-heptane. Quantitative determination by fluorescence measurement at 366/>400 nm. The method was linear within the range of 100-1000 ng/mL for both CPT-11 and TPT. The method was validated for accuracy, precision, LOD, and LOQ.

Abstract GP-18, IPC (2005). HPTLC of nimesulide and chlorzoxazone in tablets on silica gel (prewashed with methanol) with toluene - acetone - ammonia 50:50:4. Paracetamol was used as internal standard. Quantitative determination by absorbance measurement at 265 nm. Nimesulide, chlorzoxazone and paracetamol showed hRf values of 80, 73 and 42, respectively. Linearity was obtained between 0.2-1.0 mg/mL with recovery rates of 99.6-100.3 % for both compounds.The method was validated for accuracy, precision, linearity, LOD, and LOQ.

Abstract D-12, IPC (2005). HPTLC of methanol and ethyl acetate extracts of trigonella foenum graecum on silica gel with n-propanol - ammonia 11:9. Detection by spraying with ninhydrin reagent. Quantitative determination by absorbance measurement and in visible range. The hRf value of isoleucin was 60. Methanolic extracts contained 0.17 % isoleucin and ethyl acetate extracts 0.008 %. Accuracy, precision, linearity of the method were established.

J. Planar Chromatogr. 19, 32-41 (2006). TLC of 11 new derivatives of 1,2,4-triazole and of 18 new derivatives of thiosemicarbazide on RP18 with mixtures of methanol, acetonitrile, and water. Amounts of organic modifiers were in the range of 20-70 % in 10 % increments. After development in horizontal DS chambers and drying, the plates were visualized under UV light at 254 nm.

Abstract GP-74 IPC (2005). HPTLC of etoricoxib in tablets on silica gel with toluene - 1,4 dioxane - methanol 17:2:1. Quantitative determination by absorbance measurement at 235 nm. Linearity range was 500-1500 ng/spot. For stability studies the sample was subjected to acid and alkali hydrolysis, thermal, oxidaline and photo degradation. The peaks of all the degraded products were well resolved with significant different Rf values. The method was validated for different parameters and found suitable for routine quality control.

Chem. Pharm. Bull. 53, 691-693 (2005). HPTLC of trans-resveratrol in the roots of Polygonum cuspidatum and in dosage forms on silica gel with chloroform – ethyl acetate – formic acid 25:10:1. Quantitative determination by absorbance measurement at 313 nm. Linearity of determination of trans-resveratrol is between 0.5 and 3.0 µg with a correlation coefficient of 0.9989. LOD is 9 ng, and LOQ is 27 ng. The average percentage recovery is 99.9 - 100.7 %.