J. Planar Chromatogr. 23, 134-136 (2010). TLC of sertraline on silica gel (prewashed with methanol) with chloroform - ethyl acetate - triethylamine 25:15:1 in a twin-trough chamber previously saturated for 10 min at room temperature. Quantitative determination by absorbance measurement at 279 nm. The hRf for sertraline was 40. Intra-day precision and inter-day precision was 99.84 % RSD and 99.92 % RSD, respectively. A good linear relationship between response (peak area) and amount was obtained over the range 2.7-7.9 µg/band.

J. Planar Chromatogr. 23, 119-122 (2010). HPTLC of artemether and lumefantrine on silica gel with n-hexane - ethyl acetate 4:1 at room temperature in a twin-trough chamber saturated for 15 min. Quantitative determination by densitometric scanning in reflectance mode at 357 nm. The method is linear (r2 > 0.995), precise (RSD < 2 %), accurate (average recovery of 100.5 % for artemether and 99.5 % for lumefantrine), specific, and robust. LOD and LOQ for artemether were 50 and 150 ng/band, respectively, and those for lumefantrine were 300 and 900 ng/band, respectively.

Asian Journal of Chemistry 22(6), 4209-4213 (2010). HPTLC of beta-blockers on silica gel with toluene - ethyl acetate - acetone - 25 % ammonia 10:20:20:1. The method was applied to biological samples (urine and blood). The drugs were extracted from biological samples by liquid-liquid extraction. Detection at 254 nm. The corresponding separated spots were scraped from the plate, extracted with methanol and analyzed by mass spectrometry. Finally the identity was confirmed by comparing the spectra with a data library. The proposed HPTLC method coupled with MS is very sensitive and highly suitable for biological samples.

Planta Med. 76, 474-480 (2010). HPTLC of camptothecin and plant material extracts (roots, stems, leaves,and fruits) on silica gel with chloroform - ethyl acetate - methanol 9:10:1 in a twin trough chamber at 25 +/- 2°C and 40 % relative humidity. Quantitative determination by densitometry at 360 nm. Linearity was between 80 and 480 ng/zone with a correlation coefficient of 0.998 +/- 0.020. Instrumental precision (%RSD) was 0.5 %. Repeatability (%RSD) of sample and standard were 1.1 and 1.0 %. The LOD and LOQ were found to be 40 and 80 ng/zone, respectively. The accuracy of the method was proven by the average percentage recovery of 99.1 %.

J. Planar Chromatogr. 24, 44-47 (2011). HPTLC of nitrazepam on silica gel with benzene - ethanol 5:1 in a horizontal chamber with saturation for 50 min. Quantitative determination by densitometry in absorbance mode at 196 nm. The hRf of nitrazepam was 68. Linearity was in the range of 0.25-10.0 µg/zone. Mean recovery was 98.8 % and 98.8 % for tablet and pure powder, respectively. Precision and accuracy (%RSD) were 1.3 % and 1.2 %, respectively. LOD and LOQ were 0.49 and 1.52 µg/mL, respectively.

J. Planar Chromatogr. 25, 77-80 (2012). HPTLC of gemcitabine on silica gel with toluene - methanol - chloroform 6:6:5. Quantitative determination by absorbance measurement at 268 nm. The hRf value of gemcitabine was 48. Linearity was in the range of 500-3000 ng/band. Intraday and interday precisions (%RSD) were 1.4-1.6 % and 1.6-1.8 %, respectively. Recovery was found to be in the range of 98.3-101.8 %.

J. Planar Chromatogr. 25, 368-373 (2012). HPTLC of rebamipide on silica gel with toluene - methanol - triethylamine 16:9:1. Quantitative determination by absorbance measurement at 230 nm. The hRf of rebamipide was 59. Linearity was in the range of 100-600 ng/band. Limits of detection and quantification were 6 and 18 ng/band, respectively. Intermediate intra-day/inter-day precision was below 1.7 % (n=3). Recovery (by standard addition) was between 100.1 and 100.9 %. The proposed HPTLC method is equivalent to a reported HPLC method.

J. Liq. Chromatogr. Relat. Technol. 36, 2446-2462 (2013). HPTLC of amitriptyline in tablets on silica gel with acetone - ammonium hydroxide 99:1. Quantitative determination by absorbance measurement at 254 nm. The hRf value of amitriptyline was 46. Linearity was 0.70-1.30 µg/zone. The earlier model TLC to HPTLC-densitometry transfer procedure was expanded by adding sample peak identification confirmation and peak purity (specificity) tests.