GIT Fachz. Lab. Spez. Chrom. 2/96, 126-129 (1996). Combination of conventional TLC, with subsequent color detection and HPTLC, using AMD and densitometry. - HPTLC screening of amines after reductive cleavage of azoic dyes on silica with dichloromethane - acetonitrile 25:3; detection by coupling with (1-naphthyl)-ethylene diammonium dichloride. Confirmation of positive samples by AMD using a 15-step gradient. Quantification by densitometry at 200, 230, and 300 nm. As alternative TLC with diisopropylether - pentane - diethylamine 12:8:1.

CBS 88, 7 (2002) HPTLC of E 110 Yellow orange S, E 104 Quinoline yellow, E 124 Cochineal red, E 132 Indigotine I, E 151 Brilliant black BN on RP-18 W with methanol - 5 % aqueous sodium sulfate solution 3:4. Identification based on Rf-values, documentation under white light.

J. Sep. Sci. 30, 3311-3315 (2007). HPTLC of lawsone in the leaves of Lawsonia alba on silica gel with chloroform – methanol 17:3. Quantitative determination by absorbance measurement at 334 nm. The hRf value of lawsone was 40 and selectivity regarding matrix was given. Linearity was between 100 and 1000 ng/zone. The precision was 1.72 % and recovery (by standard addition) was 98.8 %.

Chinese Bulletin of Biol. 47 (7), 44-46 (2012). Chloroplast pigments play an important role in plant photosynthesis and become a kind of main raw material widely applied in the preparation of antitumor photodynamic drugs and functional foods. Phytoxanthin has outstanding function in vision protection, reducing the incidence of cataracts, anticancer therapy, and delaying the occurrence of arteriosclerosis. As a demonstration experiment for high school students analysis of chloroplast pigments by TLC and column chromatography. TLC of the extracts of fresh spinach leaves on silica gel with petroleum ether (30-60 °C) – benzene – acetone 7:1:5, detection in daylight, identification of beta-carotene (orange), chlorophyll a (dark green), chlorophyll b (light green) and phytoxanthin (bright yellow) by fingerprint comparison with the standards. Preparation by column chromatography on silica gel eluted with the same sovent system, collection of fractions, and the pure products were obtained by solvent evaporation. The results indicate that the TLC method is simple, sensitive, easy to operate, more intuitive, robust and more suitable for the purpose.

J. Chromatogr. 408, 181-190 (1987). TLC of six chlorophylls and derivatives on RP-18 silica with single solvents including alcohols, acetone, acetonitrile, and binary solvents such as acetone - acetonitrile and ethanol - water. Detection by densitometry at 410 nm and 435 nm. Discussion of the retention behaviours. Also, HPLC.

Biochemical Systematics and Ecology 18, 307-316 (1990). TLC of canthaxanthin, native xanthophylls, peridinin and peridininol on silica – CaCO3 1:1 with petrol ether – acetone – isopropanol 69,5:25:4:1,5, or on silica – Ca(OH)2– MgO – CaSO4 10:4:3:1 with petrol ether – acetone – benzene – isopropanol 69.5:25:2:1.5.

(Hungarian). Acta Pharmaceutica Hungarica 64, 117-122 (1994). TLC of anthocyanins on cellulose with formic acid - hydrochloric acid - water 6:1:5. Densitometry.

J. Agric. Food Chem. 48, 825-828 (2000). TLC of pigments on silica gel with solvent system A (light petroleum ether 40 608C) for the separation of b-carotene and lycopene, system B (petroleum ether (65 - 958C - acetone - diethylamine 10:4:1 for e.g. rubixanthin, gazaniaxanthin, b-cryptoxanthin, zeaxanthin, violaxanthin, antheraxanthin, g-carotene. Detection by observation under white and UV light.