Two new modified trichothecenes from Fusarium sporotrichioides MC-72083. Journal of Natural Products 50, 897-902 (1987). TLC of 8a- and 8ß-hydroxysambucoin on silica with benzene - acetone 3:2. Vizualization with two reagent systems: 1) 0.5% p-anisaldehyde in methanol - acetic acid - sulfuric acid 85:15:5 and 2) 1% 4-(p-nitrobenzyl)-pyridine in carbon tetrachloride - chloroform 3:2 followed, after heating for 30 min. at 150 °C., with 10% tetraethylenepentamine in carbon tetrachloride - chloroform 3:2. The epoxide-containing trichothecenes give a skyblue color.

Plant Science 54, 237-243 (1988). TLC on silica with chloroform - methanol 14:1 and toluene - ethyl acetate 1:2. Detection with anisaldehyde - sulfuric acid - acetic acid - ethanol reagent.

J. AOAC Int. 73, 270-275 (1990). TLC of zearalenone, a-zearalenol and ß-zearalenol on silica with chloroform – ethanol 97:3, detection under UV, by spraying with aluminium chloride (20 % in ethanol ) and re-examination under long-wave UV. TLC of trichothecenes (T-2 toxin, HT-2 toxin, nivalenol, fusarenon-X, neosolaniol, and deoxynivalenol) on silica with chloroform – acetone 3:2. After drying detection by spraying with 4-(p-nitrobenzyl)pyrindine, heating at 150 °C for 30 min., cooling and spraying with tetraethylenepentamine solution. New sensitive HPTLC method.

Determination of ochratoxin A in cereals, milling products from cereal and green coffee. MSZ (Hungarican Norm) 337/3 (1990). TLC of ochratoxin A on silica with benzene – methanol – acetic acid 18:1:1. Detection under UV 366 nm.

J. AOAC Int. 77, 939-941 (1994). TLC of zearalenone on silica with chloroform - acetone 9:1. Detection under UV 254 nm. Visual comparison of test spot with standard.

Chromatographia 47, 215-218 (1998). HPTLC on silica gel with toluene - ethyl acetate - formic acid 6:2:1. Quantitation by fluorodensitometry at 313 nm. Validation of the method by spiking uncontaminated extracts of maize with zearalenone over the range 10 to 320 mg/kg, with a linearity range between 10 and 80 mg/kg. Detection limit 2.6 mg/kg. Recovery > 62.8% (n=5).

J. Chromatogr. A 904 (2), 263-268 (2000). Presentation of a simple miniaturized and lower power consuming (battery, fully semiconductor based) detector cell (SeBaDeC) for the densitometric measurement of aflatoxins on TLC plates, applying an UV-light emitting diode (UV-LED) with a peak emission wavelength of 370 nm for fluorescence excitation, while a photo diode with a peak sensitivity of 440 nm in combination with a 418 nm cut-off filter is used for detecting the fluorescence intensity. Amplification of the resulting signal by means of an operational amplifier integrated circuit (OA), and direct conversion into digital signal with a ADC. Recording the signal of the serial RS232 port of a portable PC and processing it with a spreadsheet program. Detection level as low as concentration of aflatoxins of 1 ng.

J. Planar Chromatogr. 23, 193-197 (2010). HPTLC of ochratoxin A, aflatoxin B1, G1, B2, and G2 on silica gel with t-butyl methyl ether - water - methanol - cyclohexane 48:1:2:1 in an unsaturated horizontal developing chamber and on RP18 with methanol - 4 % aqueous zinc sulfate solution - ethyl methyl ketone 5:5:1. After development the silica gel plate was dipped for 2 s in silicone oil - hexane 1:2 which enhanced aflatoxin fluorescence by a factor of 2 and ochratoxin A fluorescence by a factor of 3-10. RP18 plates were developed to a distance of 75 mm in an unsaturated vertical chamber. Averaged densitograms were obtained in the emission wavelength range from 445 to 485 nm. Sample pretreatment was by modified QuEChERS (Quick, Easy; Cheap, Effectice, Rugged, Safe) extraction with tetahydrofuran or acetone. Linearity was in the range of 3 to 100 pg/zone for aflatoxins B2 and G2, 10 to 350 pg/zone for aflatoxins B1 and G1, and 0.25 to 2.5 ng/zone for ochratoxin A. LOQ for the aflatoxins were between 13 and 35 pg/zone (equivalent to to 1.5 and 2.5 ppb); for ochratoxin A it was 970 pg/zone (56 ppb).