J. Planar Chromatogr. 28, 119-125 (2015). The goal of this quantitative structure-retention relationship study was to investigate the retention behavior and to characterize the lipophilicity of 16 guanidine and imidazoline derivatives by TLC on RP-8 with methanol–water–ammonia (in different volume ratios), simulating the n-octanol–water partition system. Detection by absorbance measurement at 254 nm. Three different modeling methodologies (stepwise MLR, PLR, ANN) were tested. The stepwise MLR could be used as the most reliable model for prediction of retention behavior in the examinated RP-TLC system.

Chinese J. Med. Industry 17, 422-423 (1986) (Yiyao Gongye). TLC on silica with butanol - isopropanol - 38% NH3 3:1:0.5. Quantification by densitometry at 460 nm.

J. Chromatogr. 499, 361-371 (1990). Studies of reverse-phase retention behavior of 18 benzodiazepine derivatives using TLC on paraffin-impregnated silica with a solution containing 0 – 32.5 Vol-% of acetonitrile in steps of 2.5 % and 6, 12, 30, 60, and 90 mM KHPO4 solution. Detection by their UV absorption spectra. Linear dependence of RM value on organic phase concentration and logarithmically on buffer concentration in mobile phase. Prediction of HPLC retention behavior.

Anal. Letters 27, 723-729 (1994). TLC of phenytoin on silica with chloroform - isopropylether - methanol - NH3 75:25:20:1. Quantification by densitometry at 254 nm.

J. Planar Chromatogr. 15, 38-41 (2002). Study of the effect of impregnating TLC plates with some synthetic peptides and aspartic acid on the speed of migration of 3-(w-aminoalkyl)-5,5-dialkyl-(or spirocycloalkyl-1',5)hydantoin derivatives of phenylpiperazines and comparison of the results with the affinity of the impregnants for 5-HT 1A and 5-HT 2A receptors. TLC of 10 5-HT 1A and 5-HT 2A compounds on silica gel impregnated with H-Asp-OH, H-Asp-Ile-OH, H-Leu-Asp-Val-Leu-OH, and H-Ala-Asp-Val-Leu-OH with chloroform - methanol 10:1. Visualization under UV and by use of an iodine chamber.

Indian J. Pharm. Sci. 72(2), 249-252 (2010). HPTLC of rosiglitazone on silica gel with methanol - toluene - chloroform - triethyl amine 2:161:1. Caffeine was used as internal standard. The hRf value of rosiglitazone was 31 and of caffeine 52. Densitometric evaluation at 264 nm. The method was linear in the range of 5-50 µg/band with average recovery of 99.8-100.2 %. The method was suitable for content uniformity testing according to USP and could be used for stability studies as well.

62nd Indian Pharmaceutical Congress Abstract No. F-258 (2010). TLC of nebivolol HCl and hydrochlorothiazide on silica gel with methanol – chloroform – toluene – triethylamine 10:25:14:1. Quantitative determination by absorbance measurement at 284 nm. The hRf value of nebivolol was 78 and of hydrochlorthiazide 41. The method was linear in the range of 5-100 ng/band and 20-140 ng/band for nebivolol HCl and hydrochlorothiazide, respectively. Recovery was in the range of 98.8-100.0 % for both drugs.

J. Ethnopharmacol. 175, 131-137 (2015). HPTLC of 11-hydroxymustakone (1), N-formylannonain (2) and yangambin (3) in Tinospora cordifolia on silica gel with chloroform - ethyl acetate - methanol 130:80:3. Detection by dipping into anisaldehyde sulfuric acid reagent, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 366 nm. LOD and LOQ were 75 and 225 ng/zone for (1), 150 and 450 ng/zone for (2) and 30 and 90 ng/zone for (3), respectively. The intermediate precision was below 3 % (n=3). Recovery for (1) to (3) was in the range of 99-108 %.