Effect of replacing a piperazine ring with an alkanediamine moiety on a, -adrenoreceptor blocking activity. J. of Medicinal Chemistry 32, 50-55 (1989). TLC on silica with petrol ether - ethyl acetate - methanol - NH3 28% 15:35:10:1, chloroform - petrol ether - methanol - NH3 28 % 80:80:15:1, petrol ether - ethyl acetate - methanol - NH3 28% 40:30:10:1, chloroform - petrol ether - methanol - NH3 28% 25:15:5:0. 4, petrol ether - ethyl acetate - methanol - NH3 28% 60:90:10:1, ethyl acetate - cyclohexane 1:1. Visualization under UV 254 nm.

Phytochemical Analysis 3, 218-222 (1992). TLC of numerous plant hormones on silica with a chemically bonded C-2 substituent using methanol - water in different proportions and containing 0.7 N NH3. Also TLC on silica impregnated with liquid paraffin and with the silicone fluid dimethylpolysiloxane. Development with aqueous methanol solvents containing NH3. Detection under UV. Systematic study of numerous cytokinins by TLC. Description of excellent chromatographic conditions for the separation of closely related plant hormones.

2. Benzoquinoxalinone derivatives. J. Planar Chromatogr. 8, 444-449 (1995). TLC and HPTLC of 3-substituted benzo(g)quinoxalinone derivatives on silica with dichloromethane - ethyl acetate 7:3. Detection under UV 254 and 366 nm.

J. Planar Chromatogr. 17, 255-260 (2004). TLC of fourteen 1-arylpiperazine derivatives on silica gel with monocomponent and binary non-polar mobile phases and on RP-18 with binary mobile phases comprising mixtures of methanol, acetone, or dioxane (as organic modifiers) and water in a horizontal chamber after equilibration for 15 min. Detection under UV light at 254 nm.

J. Planar Chromatogr. 23, 56-64 (2010). HPTLC of thirteen quinolines (quinoline, isoquinoline, 2,4-dihydroxyquinoline, 8-hydroxyquinoline, 5,6-benzoquinoline, 2,2’-diquinoline, 8-methylquinoline, 5-aminoquinoline, 1-ethylquinoline, 2-chlorquinoline, 6-nitroquinoline, 2,6-dimethylquinoline, 5-hydroxyquinoline) on cyano phase with different mobile phases prepared from mixtures of methanol, acetonitrile, 2-propanol, tetrahydrofuran, and dioxane with addition of buffer at different pH, ion-pairing reagents, or silanol blockers. Detection under UV light at 254 nm and by densitometric absorbance measurement at 254 nm. Improved peak symmetry was observed for mobile phases containing ion-pairing reagent. The most symmetrical peaks were obtained by use of mobile phase containing diethylamine as silanol blocker.

62nd Indian Pharmaceutical Congress Abstract No. F-263 (2010). TLC of desloratadine on silica gel with methanol – chloroform – toluene – ammonia 50:50:10:3. Quantitative determination by absorbance measurement at 254 nm. The hRf value was 61. The linearity was in the range of 150-750 ng/zone with r2 = 0.9997. The limit of detection was 21 ng/zone, whereas the limit of quantitation was 65 ng/zone.

J. Chil. Chem. Soc. 58, 1663-1666 (2013). HPTLC of rosiglitazone (1) and glimepiride (2) in tablet dosage form on silica gel with methanol - toluene - ethyl acetate 1:8:1. Quantitative determination by absorbance measurement at 228 nm. The hRf values for (1) and (2) were 39 and 20, respectively. Linearity was in the range of 100-1500 ng/zone for (1) and 100-1500 ng/zone for (2). LOD and LOQ were 30 and 35 ng/zone for (1) and 85 and 90 ng/zone for (2), respectively. Recovery was 96 % for (1) and (2). Intermediate intra- and inter-day precision was below 2 % (n=6).

Saturated heterocycles, 51. Synthesis of stereoisomeric 1,3- thiazines with condensed skeleton. Acta Chimica 118, 37-42 (1985). TLC of cis- and trans-4,5- tetramethylene-4,5- dihydro-6H-1,3-thiazines on silica with butanol - ether - acetic acid 1 0:10:1.