J. Liq. Chromatogr. Relat. Technol. 33, 109-117 (2010). TLC of ursodeoxycholic acid on silica gel with n-hexane - ethyl acetate - acetic acid 22:22:5 at room temperaure in a horizontal chamber. The hRf value was 48. Detection by spraying with 10 % sulfuric acid and heating at 120 °C for 20 min. Quantitative determination by densitometric scanning at 360 nm. LOD and LOQ was 0.014 and 0.041 mg/spot, respectively. The linearity range was 0.030-0.120 mg/spot. The correlation coefficient r was 0.0063. The accuracy of the quantitative analysis of ursodeoxycholic acid in extracts from examined pharmaceutical formulations was 95.3 % and 97.2 %, and the precision was 5.29 and 6.15 % in tablets and capsules, respectively.

Ind. J. Pharma. Science 71 61, 644-650 (2009). HPTLC of the novel antihyperlipidemic agent LM-13765 in rabbit plasma on silica gel (pre-washed with methanol) with benzene - methanol 4:1 in a saturated chamber at 25 °C. Densitometric evaluation at 314 nm. The method was linear in the range of 12.5-100 ng/band. Also HPTLC of LM-13765-C, a metabolite of the parent compound LM-13765, on silica gel with toluene - methanol 23:2. Densitometric evaluation at 274 nm. Details are provided on the complete extraction procedure for the extraction of the parent compound and its metabolite from plasma.

J. Planar Chromatogr. 15, 299-305 (2002). TLC of 15 cholic acid derivatives on 9 reversed-phase and 3 normal-phase systems (RP-18, cyano-, polyacrylonitrile-modification and unmodified silica gel) with 9 different mobile phases. Detection by spraying with 50% sulfuric acid and heating. RP-18 combined with mobile phases containing 60-70% methanol or 50-60% dioxane in water can be recommended as the most suitable chromatographic system for the separation of cholic acid derivatives.

J. Planar Chromatogr. 18, 465-470 (2005). HPTLC of cholic, glycocholic, glycodeoxycholic, chenodeoxycholic, deoxycholic, lithocholic, and glycolithocholic acid on RP-18 W, RP-2, and cyano phases in a presaturated chamber with mixtures of an organic modifier (methanol, dioxane, acetonitrile, acetone) and water in different volume proportions which were varied in steps of 5 % from 35 to 80 %. Detection by spraying with a 10 % aqueous solution of sulfuric acid or by dipping in a 10 % solution of phosphomolybdic acid in ethanol and heating at 120 °C for 20 min. Investigation of relationships between lipophilicity obtained by use of RP-HPTLC, experimental and theoretical partition coefficients, and selected structural descriptors.