Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      87 054
      Three-step purification of a fragment of the large immunophilin FKBP52
      F. PIRKL, (Inst. Org. Chem & Biochem., Tech. Univ. München, Lichtenbergstrasse 4, DE-85747 Garching, Germany)

      J. Chromatogr. B 737 (1/2), 71-77 (2000). Description of a fast and effective three-step purification procedure for a fragment of FKBP52, consisting of affinity chromatography, anion-exchange and gel-permeation chromatography. Verification of the purity of the products by SDS-PAGE.

      Keywords:
      Classification: 19, 38
      111 011
      Introduction of special HPTLC and TLC plates for coupling with mass spectrometry
      M. SCHULZ*, B. SCHUBACH, Susanne MINARIK, H. GRIESINGER (*Merck KGaA, MM-LER-CP. Frankfurterstr. 250, 64293 Darmstadt, Germany, michael.schulz@merckgroup.com)

      CBS 110, 10-11 (2013). New HPTLC silica gel plates specially suited for coupling with MS and TLC plates for coupling with NMR. These MS-grade HPTLC plates are low in impurities and show less background noise, the layer thickness is 100 µm. HPTLC of human insulin and its secondary components on MS-grade silica gel with 2-butanol - pyridine - ammonia 25 % - water 39:34:10:26, detection under UV 366 nm after treatment with fluorescamine and by elution with the TLC-MS Interface and Q-TOF MS analysis in ESI positive mode, the eluent was acetonitrile - water 19:1. Separation and identification of insulin and desamido insulin was achieved, even though there is only 1 Da mass difference.

      Keywords: HPTLC
      Classification: 3b, 19
      70 115
      Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes
      S. BIRNBAUM*, C. UDEN, C.G.M. MAGNUSSON, S. NIELSSON, (*Dep. Pure & App. Biochem. Univ. Lund, P.O. Box 124, 2400 Lund, Sweden)

      Anal. Biochem. 206, 168-171 (1992). Description of a rapid immunochromatographic method for the analysis of protein antigents, based on „sandwich“ assay format using monoclonal antibodies of two distinct specificities, one covalently immobilized to a defined detection zone on a porous membrane while other serve as a label. The sample is mixed with the coating, and the mixture is then passed along a porous membrane by capillary action past the antibodies in the detection zone, giving a blue color. Detection limit, below the nanomolar range for the antigen, human chlorionic gonadotropin.

      Keywords:
      Classification: 3b, 19
      76 222
      Advances in affinity electrophoresis
      K. TAKEO, (Yamaguchi Prefectural Public and Nursing Coll., Izumi-machi 21-1, Hofu-shi 747, Japan)

      J. Chromatogr. 698, 89-105 (1995). A review with 54 references on the basic principles of affinity electrophoresis and quantitative affinity electrophoresis with the latest applications, especially the thermodynamic analysis of biospecific interactions by affinity electrophoresis and the development of two-dimensional affinity heterogeneity, lectin affinity electrophoresis for characterizing glycoprotein and newly developed capillary gel affinity electrophoresis.

      Keywords: review
      Classification: 19, 36
      87 055
      Chromatographic purification of the CH2 domain of the monoclonal antibody MAK 33
      M.J.W. THIES*, F. PIRKL, (*Inst. Org. Chem. & Biochen., Techn. Univ. M¸nchen, Lichtenbergstrasse 4, DE-85747 Garching, Germany)

      J. Chromatogr. B 737 (1/2), 63-69 (2000). The title protein was expressed in Escherichia coli forming insoluble inclusion bodies (Ibs) and purified in a three-step, hydrophobic interaction and gel permeation chromatography. Determination of the purity of the CH2 protein by a silver-stained SDS-PAGE.

      Keywords:
      Classification: 19, 38
      111 036
      Analysis of insulin samples from different species by HPTLC-MS
      M. SCHULZ*, Susanne MINARIK, B. SCHUBACH, I. FAHR (*Merck KGaA, MM-LER-CP. Frankfurterstr. 250, 64293 Darmstadt, Germany, michael.schulz@merckgroup.com)

      CBS 108, 4-5 (2012). HPTLC of human insulin recombinant and porcine and bovine pancreas insulin on ProteoChrom silica gel with 2-butanol - pyridine - 25 % ammonia - water 39:34:10:26 to 50 mm migration distance. Detection under UV 366 nm after spraying with 0.02 % fluorescamine in acetone. Elution with the TLC-MS Interface and MS analysis in ESI positive mode, the eluent was acetonitrile - water 1:1. The hRf value of human, porcine and bovine insulin was 50. All mass spectra could be clearly assigned to the different insulin samples.

      Classification: 19
      70 116
      (Analysis of the composition changes in protein and lipid of modified keratin fibers
      Y. DENG (Deng Yanru), J. MENG (Meng Jinjun) (Xinjiang Inst. Chem., Academia Sinica, Ulumuqi 830011, P.R. China)

      J. China Assoc. Instr. Anal. (Fenxi Ceshi Tongbao) 11, 74-77 (1992). TLC of the composition of protein and lipid in chemically modified keratin fibers on silica with chloroform - methanol 80:2. Detection by exposure to iodine vapor. Also polyacrylamide gel electrophoresis.

      Classification: 11c, 19
      76 223
      Current status and perspectives of computer-aided two-dimensional densitometry
      T. TODA*, M. OHASHI, (*Dept. Mol. Biol., Tokyo Metropolitan Inst. Gerontol., 35-2 Sakaecho, Itabashi-Ku, Tokyo 173, Japan)

      J. Chromatogr. 698, 41-54 (1995). A review with 35 references on the architectures of hardware and algorithms of software that have been developed for two-dimensional densitometry. Discussion of the quantitative aspects of two-dimensional densitometry and the perspective of two-dimensional gel protein databases.

      Keywords: review
      Classification: 4c, 19, 36