Anal. Chem. 79, 486-493 (2007). TLC of methylene blue and methyl red on monolithic phase with ethyl acetate - ethanol -water 6:4:3 and 3:2:1 with chamber saturation for 30 min. After development the plates were dried and scanned with MALDI. TLC separation of fluorescamine labeled proteins (insulin, cytochrome c, lysozyme, and myoglobin) with 40 or 55 % aqueous acetonitrile and 0.1 % trifluoro acetic acid with chamber saturation for 30 min. Detection under UV 366 nm. Preparation of the monolithic layer: The polymerization mixture consisted of butyl methacrylate, ethylene dimethacrylate, 1-decanol, cyclohexanol, and 2,2-dimethoxy-2-phenyl-acetophenone.

(Thin-layer chromatographic enantiomeric resolution via ligand exchange.) GIT-Suppl. 3, 6-12 (1986). Separation of enantiomeric amino acids and dipeptides on reversed phase silica gel covered with a chiral selector (proline derivative). Typical developing solvents: methanol - water - acetonitrile 5:5:20 (running time 30 min) and 5:5:3 (running time 90 min).

1. Polar pentapeptides. J. of Medicinal Chemistry 31, 960-966 (1988). TLC of enkephalin-related pentapeptides on silica with chloroform - methanol 8:1, chloroform - methanol - 32% acetic acid 120:90:5, chloroform - methanol - NH3 120:90:5 or 120:90:40, n-butanol - acetic acid - water 3:1:1 and chloroform - methanol 95:5. Visualization under UV and/or by spraying with ninhydrin and other reagents.

J. of Medicinal Chemistry 32, 807-826 (1989). TLC of peptidoleukotrienes on silica or C-18 reversed-phase silica with dichloromethane - hexane 5:95 and methanol - water 1:1. Visualization under UV and/or by spraying with molybdic acid - sulfuric acid followed by charring.

J. Chromatogr. 553, 467-475 (1991). Determination of the retentions of 18 peptides in reverse-phase TLC with methanol as the organic mobile phase. Preadsorption of inorganic salts (LiCl, NaCl, KCl, CaCl2 and AgCl2) on silica. Investigation of the effect of the salts on the silanophilic effect, and elucidation of the role of the salt concentration, that of ion charge, and hydrated ion radii.

J. Planar Chromatogr. 12, 150-151 (1999). OPLC of 5 synthetic model peptides (e.g. Ac-Pro-Val-Val-Ser-Gly-NH2, Ac-Pro-Glu-Val-Ala-Gly-NH2) on silica gel with butanol - pyridine - acetic acid - water 12:4:1:4. After thorough drying the plates were treated with chlorine-toluidine reagent.

J. Chil. Chem. Soc. 58, 1737-1740 (2013). HPTLC of enalapril maleate in tablets on silica gel with 1-buthanol - glacial acetic acid - water 12:3:5. Quantitative determination by absorbance measurement at 207 nm. The hRf values of enalapril and its degradation products enalapril diketopiperazine and enalaprilat were 62, 82 and 52, respectively. Linearity was in the range of 200-1200 ng/zone for enalapril. LOD and LOQ were 23 and 72 ng/zone. Intermediate intra- and inter-day precision was below 2 % (n=6).

J.A.O.A.C. 69, 938-940 (1986). TLC separation of actaplanin on cellulose with methanol - chloroform - NH3 55:15:35. Detection by spraying with agar medium containing a diluted B. subtilis spore suspension, incubation over night at 37 °C and spraying with 2 mg/ml aqueous solution of INT.