Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      81 057
      Analysis of amino acids by personal OPLC instrument
      E. TYHIAK*, G. KATAY, Z. OSTORICS, E. MINCSOVICS, (*Plant Prot. Inst., Hungarian Acad. of Sci., Budapest, P.O. Box 102, H-1525 Hungary)

      Separation of the main protein amino acids in a double-layer system. J. Planar Chromatogr. 11, 5-11 (1998). Demonstration of the theoretical aspects of the long-distance development, the basic elements of the double layer cassette for personal OPLC instrument, and the first results in the field of amino acid analysis with special emphasis on the 20 main protein amino acids. HPTLC on single and on double silica gel plates with butanol - acetonitrile - 0.005 M potassium dihydrogen phosphate - acetic acid 1:5:3:1. Detection after treatment with ninhydrin reagent at 490 nm.

      Keywords: densitometry
      Classification: 18a
      86 037
      Quantitative analysis of L-tryptophan in fermentation broth
      E.V. DEGTEREV, W.G. DEGTIAR, B.V. TYAGLOV, A.P. TARASOV, V.M. KRYLOV, I.I. MALAKHOVA, V.D. KRASIKOV, (The Center for Chem. of Drugs, The All-Russian Res., Chemico-Pharm. Inst., Zubovskay str. 7, 119815 Moscow, Russia)

      J. Planar Chromatogr. 13, 191-194 (2000). Quantitative TLC determination of L-tryptophan on silica gel with 2-propanol - 25 % NH3 7:3. Visualization of amino acids by dipping into a solution of 300 mg ninhydrin in acetone/acetic acid 97:3. Detection of L-tryptophan by treatment with 4-dimethylaminobenzaldehyde and heating at 110°C for 7-10 min. Quantitation by densitometry at 625 nm. Rapid method for determination of L-tryptophan.

      Keywords:
      Classification: 18a
      93 060
      Quantification of arginine in dietary supplement tablets and capsules by silica gel high performance thin-layer chromatography with visible mode densitometry
      B. HESS, J. SHERMA* (*Dep.Chem., Lafayette College, Easton, P.A. 18042, USA)

      Acta Chromatographica 14, 60-69 (2004). TLC on silica gel with 1-butanol - glacial acetic acid - water 3:1:1 with chamber saturation. Detection by spraying with ninhydrin reagent (0.3 g ninhydrin in 100 mL 1-butanol plus 2 mL glacial acetic acid), followed by heating at 110 ºC for several min. Quantification by densitometry at 495 nm. Validation of the accuracy by analysis of spiked blank and standard addition samples and precision by performing replicate analysis on a single day and on different days. Recoveries and RSD for spiked blank and standard addition samples were 98.8 % and 98.5 %, 1.92 % and 0.67 %, respectively. Discussion of use of the method for the routine quality control of nutritional supplements.

      Classification: 18a
      102 062
      Identification and quantification of amino acids in peptides
      Roseline SBAFFO-POASEVARA (API Analytical Development, IPSEN, 5 avenue du Canada, 91966 Les Ulis, France, roseline.poasevara@ipsen.com)

      CBS 101, 2-4 (2008). HPTLC of amino acids (from hydrolysis of peptides) on silica gel, Diol phase, and cellulose with either 2-butanol - acetic acid - pyridine - water 15:3:10:12 or 2-butanol - 25 % ammonia - pyridine - water 39:10:34:26 in a twin-trough chamber or horizontal chamber. Detection by dipping in ninhydrin solution (0.5 % in 2-propanol) followed by heating at 110 °C for 5 min. Better results are achieved by adding ninhydrin directly to the mobile phase at a 0.5 %-level. Quantitative determination by absorbance measurement at 440 nm. Selectivity was better on the cellulose and silica gel plate. Selection of the chromatographic system depended on which amino acids had to be separated and no general recommendation could be given. HPTLC analysis of a hydrolyzed peptide sample (containing Phe, Trp, D-Bal, Apc and Inp) on cellulose with the acidic mobile phase. All five amino acids were quantified between 70 and 130 % of the theoretical value for non-stable amino acids (degradation 5 to 30 %).

      Classification: 18a
      109 049
      Quantitative HPTLC analysis of palmitoyl hexapeptide
      S. SHAHI*, R. ATHAWALE (*C. U. Shah College of Pharmacy, 11/602 Mandar, Vasant Vihar Complex,Thane (W)-400 601, India; shilpa_s2000@rediffmail.com)

      J. Planar Chromatogr. 23, 365-368 (2010). HPTLC of palmitoyl hexapeptide (an antiwrinkle peptide) on silica gel with toluene - ethanol 9:1 in a twin-trough chamber with saturation for 30 min at room temperature (25 +/- 2 °C). The hRf was 33. Quantitative determination by absorbance measurement at 211 nm. Linearity was between 10 and 30 ng/band. The LOD and LOQ was 3 and 9 ng/band, respectively. The intra-day precision (%RSD, n = 6) was 0.9-1.5 % and the inter-day precision 0.9-1.4 %. The small %RSD obtained after small changes of the method conditions indicate the method is robust. The recovery of the method was in the range of 98.9-101.3 %.

      Classification: 18a
      116 012
      Establishing a method for thin-layer chromatography–pixel quantification
      Y. CHEN (Chen Yibin)*, J. XU (Xu Jin), M. WANG (Wang Min), P. LIAN (Lian Ping), Z. WANG (Wang Zhendong) (*College of Food and Biotechnology, Shaanxi University of Science and Technology, 710021, Xi’an, Shaanxi, China, chenyibin@sust.edu.cn)

      J. Planar Chromatogr. 28, 466-471 (2015). TLC of L-glutamic acid in a solution of Astragalus complanatus coarse powder on silica gel with n-butyl alcohol - glacial acetic acid - water 4:1:1. Detection by spraying with 0.2 % ninhydrin - acetone solution 10:1, followed by heating at 105 °C. Quantitative determination by measuring pixels after photographing. Linearity was in the range of 10-60 μg/zone. Average recovery was 103 %. Different measures to reduce the error were proposed.

      Classification: 3f, 18a
      55 071
      A study of changes in the free amino acid content in tomato leaves by various OPLC methods
      A. SARHAN, B. BARNA, Z. KIRALY

      Effect of nitrogen nutrition. Proc. Intern. Symposium on TLC with special emphasis on OPLC, Szeged, 75-76 (1984). OPLC (OPTLC) of proline, hydroxyproline, glutamine, asparagine on silica with 10 different buffer solutions. Detection with ninhydrin in methanol - acetic acid and heating at 105 °C for 10 minutes. Quantitative determination by densitometry.

      Classification: 18a
      58 080
      Rapid separation of enantiomers by thin-layer chromatography on a chiral stationary phase
      U.A.TH. BRINKMANN, D. KAMMINGA

      J. Chromatogr. 330

      Classification: 18a