Isolation of mycosporine hydroxyglutamicol from Nephroma laevigatum Ach. Phytochemistry 72, 1348-1357 (2011). HPTLC of mycosporines and mycosporines-like amino acids on silica gel with chloroform – methanol – water 6:4:1. The plate was dried in a stream of nitrogen and protected from light. Detection by absorbance measurement at the respective maximum wavelength between 310 and 360 nm. The hRfvalues of the compounds ranged between 20 and 80. HPTLC allowed a rapid and simultaneous comparison of 12-20 extracts for UV-absorbing compounds within 2 h.

Chinese J. Chromatogr. 32 (3), 242-247 (2014). Definition of novel molecular connectivity indices and calculation of the indices for 18 chiral hydroxyl acids and amino acids, based on Kier’s molecular connectivity indices and conjugated matrix. Study of the quantitative structure-retention index relationship (QSRR) between the retention index of the chiral organic acids and the chiral connectivity indices by multivariate statistical regression. The best four-parameter QSRR model was set up through leaps-and-bounds regression analysis. The traditional correlation coefficient and the cross-validation correlation coefficient of leave-one-out were given, respectively. The results demonstrated that the model was highly reliable and had good predictive ability from the statistical point of view. From the parameters of the model, it is known that the dominant influence factors on the retention index were the two-dimensional molecular structure characteristics and the space factors such as the chiral characteristics, the flexibility and the puckered degree of the molecules of the chiral organic acids. The results also indicated that the introduced chiral connectivity indices had good rationality and validity for the characterization of the retention indices for the chiral organic acids, thus, provided an effective method for predicting the retention indices of chiral organic acids.

varieties by analytical OPLC. Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 46 (1984). OPLC (OPTLC) of trimethyl-L-lysine on silica with propanol - methanol - 0.l M sodium acetate in water 20:3:30. Detection with Dragendorff reagent. Densitometry by absorbance at 525 nm.

V. Danube Symposium on Chromatography Yalta, November 11 -16, 1985. TLC study : the composition of intermediates in the microbiological synthesis of threonine, tryptophan and glutamic acid. TLC on silica with isopropanol - ethyl acetate - NH3 - water 20:20:1.5:25. Detection with ninhydrin.

Anal. Letters 19, 543-552 (1986). TLC of 24 amino acids on tin(IV) arseno-silicate cation exchanger with various buffer solutions. Detection by spraying with 2 % alcoholic ninhydrin and heating.

Anal. Chem., 59, 2045-2050 (1987). Investigation of the performance of several mathematical approaches for quantification of components in case of severe chromatographic and spectroscopic overlap. Demonstration by the separation of two amino acids, glycine and glutamine on silica with 1-butanol - acetone - acetic acid - water 7:7:2:4 followed by derivatization with O-phthaldialdehyde. Studies of 5 approaches based on factor analysis and Kalman filtering methods with errors of 5.9% for glycine and - 6.4% for glutamine.

J. Chromatogr. 436, 341- 343 (1988). TLC on silica with methanol - chloroform 3:1. Detection by spraying first with 0.01% fluorescein isothiocyanate in 0.1M aqueous sodium hydroxide solution and heating at 90 °C for 10 min., then with 0.25 % ninhydrin in acetone and heating again at 90 °C for 10 min. Observation under visible light and UV 280 nm. Detection limit, 0.5-1.0 µg.

Chinese J. Adv. in Biochem. & Biophys. (Shengwu Huaxue Yu Shengwu Wuli Jinzhan) 15, 452-453 (1988). Two-dimensional TLC on microcrystalline cellulose - silica 5:2 or 6:4 with ethyl acetate - acetone - methanol - parapentanol - 26% NH3 - water 9:3:3:1:1:3 for the 1st dimension and butanol - acetone - isopropanol formic acid - water 18:8:8:3:6 for the 2nd. Detection by spraying with cadmium-ninhydrin reagent.