Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      86 039
      Qualitative reversed-phase thin-layer chromatographic analysis of the stereochemistry of D- and L-a-amino acids in small peptides
      J.W. LeFEVRE*, E.J. GUBLO, C. BOTTING, R. WALL, A. NIGRO, M.-L. T PHAM, G. GANCI, (Chem. Dept., State Univ. of New York (SUNY) at Oswego, Oswego, NY 13126, USA)

      J. Planar Chromatogr. 13, 160-165 (2000). TLC of a-amino acids in small peptides; after sequential acid hydrolysis of a peptide, derivatization of the free amino acids with dansyl chloride, purification of the resulting DNS-amino acids by TLC, and stereochemical analysis by reversed-phase TLC. Purificatiom of DNS-amino acids (DNS-D-Arg, DALDA, DNS-Leu, DNS-Val) on silica gel with toluene - pyridine - acetic acid 40:10:1. After extraction stereochemical analysis on RP-18 with e.g. an aqueous solution of b-cyclodextrin containing different proportions of either acetonitrile or methanol, depending on the polarity of the DNS-amino acid. Detection by densitometry at 366 nm.

      Keywords:
      Classification: 18
      102 062
      Identification and quantification of amino acids in peptides
      Roseline SBAFFO-POASEVARA (API Analytical Development, IPSEN, 5 avenue du Canada, 91966 Les Ulis, France, roseline.poasevara@ipsen.com)

      CBS 101, 2-4 (2008). HPTLC of amino acids (from hydrolysis of peptides) on silica gel, Diol phase, and cellulose with either 2-butanol - acetic acid - pyridine - water 15:3:10:12 or 2-butanol - 25 % ammonia - pyridine - water 39:10:34:26 in a twin-trough chamber or horizontal chamber. Detection by dipping in ninhydrin solution (0.5 % in 2-propanol) followed by heating at 110 °C for 5 min. Better results are achieved by adding ninhydrin directly to the mobile phase at a 0.5 %-level. Quantitative determination by absorbance measurement at 440 nm. Selectivity was better on the cellulose and silica gel plate. Selection of the chromatographic system depended on which amino acids had to be separated and no general recommendation could be given. HPTLC analysis of a hydrolyzed peptide sample (containing Phe, Trp, D-Bal, Apc and Inp) on cellulose with the acidic mobile phase. All five amino acids were quantified between 70 and 130 % of the theoretical value for non-stable amino acids (degradation 5 to 30 %).

      Classification: 18a
      109 049
      Quantitative HPTLC analysis of palmitoyl hexapeptide
      S. SHAHI*, R. ATHAWALE (*C. U. Shah College of Pharmacy, 11/602 Mandar, Vasant Vihar Complex,Thane (W)-400 601, India; shilpa_s2000@rediffmail.com)

      J. Planar Chromatogr. 23, 365-368 (2010). HPTLC of palmitoyl hexapeptide (an antiwrinkle peptide) on silica gel with toluene - ethanol 9:1 in a twin-trough chamber with saturation for 30 min at room temperature (25 +/- 2 °C). The hRf was 33. Quantitative determination by absorbance measurement at 211 nm. Linearity was between 10 and 30 ng/band. The LOD and LOQ was 3 and 9 ng/band, respectively. The intra-day precision (%RSD, n = 6) was 0.9-1.5 % and the inter-day precision 0.9-1.4 %. The small %RSD obtained after small changes of the method conditions indicate the method is robust. The recovery of the method was in the range of 98.9-101.3 %.

      Classification: 18a
      116 012
      Establishing a method for thin-layer chromatography–pixel quantification
      Y. CHEN (Chen Yibin)*, J. XU (Xu Jin), M. WANG (Wang Min), P. LIAN (Lian Ping), Z. WANG (Wang Zhendong) (*College of Food and Biotechnology, Shaanxi University of Science and Technology, 710021, Xi’an, Shaanxi, China, chenyibin@sust.edu.cn)

      J. Planar Chromatogr. 28, 466-471 (2015). TLC of L-glutamic acid in a solution of Astragalus complanatus coarse powder on silica gel with n-butyl alcohol - glacial acetic acid - water 4:1:1. Detection by spraying with 0.2 % ninhydrin - acetone solution 10:1, followed by heating at 105 °C. Quantitative determination by measuring pixels after photographing. Linearity was in the range of 10-60 μg/zone. Average recovery was 103 %. Different measures to reduce the error were proposed.

      Classification: 3f, 18a
      55 071
      A study of changes in the free amino acid content in tomato leaves by various OPLC methods
      A. SARHAN, B. BARNA, Z. KIRALY

      Effect of nitrogen nutrition. Proc. Intern. Symposium on TLC with special emphasis on OPLC, Szeged, 75-76 (1984). OPLC (OPTLC) of proline, hydroxyproline, glutamine, asparagine on silica with 10 different buffer solutions. Detection with ninhydrin in methanol - acetic acid and heating at 105 °C for 10 minutes. Quantitative determination by densitometry.

      Classification: 18a
      58 080
      Rapid separation of enantiomers by thin-layer chromatography on a chiral stationary phase
      U.A.TH. BRINKMANN, D. KAMMINGA

      J. Chromatogr. 330

      Classification: 18a
      60 106
      Resolution of PTH-amino acids in three new solvents on Zn, Cd, Hg impregnated silica plates
      R. BHUSHAN, I. ALI (Chemistry Dept. Univ. of Roorkee, Roorkee-247667, India)

      J. Liquid. Chrom. 9, 3479-3487 (1986). TLC of 10 PTH-amino acids on silica (impregnated with zinc acetate, cadmium nitrate and mercuric chloride, 0.1, 0.2, 0.5 % concentration of each resp.) with chloroform - water - ethyl acetate 28:1:1; CCI4 - acetic acid 19:1 and chloroform - benzene - methanol 19:9:1. Detection with iodine chamber.

      Keywords:
      Classification: 18a
      61 118
      (Study of the paper and thin-layer chromatographic behaviours of 75Se-methionine)
      SH. DANG (Dang Shuqin), Q. LI (Li Qingshu), (Isotope Dep., Inst. Atom.Energy, Beijing, P.R. China)

      (Chinese). Chinese J. Chromatogr. 6, 46-48 (1988) (Sepu). Investigation of TLC behaviours of 75Se-methionine sulfoxide, 75Se-methionine sulfone and selenoxide on silica with ethanol - water in various proportions. Detection by spraying with ninhydrin reagent.

      Keywords:
      Classification: 18a