J. Planar Chromatogr. 24, 16-22 (2011). A novel method is proposed for optimization of simultaneous thin-layer chromatographic separation of seven amino acids. For this purpose a useful combination of genetic algorithms (GA) with artificial neural networks (ANN) was employed. Methods investigated in this work were successfully used for prediction of resolution (RS) and optimization of the separation of model solutions containing the seven compounds. Very good correlation was achieved between predicted and calculated RS data, and low absolute and relative errors were obtained. TLC of alanine, asparagine, cysteine, leucine, phenylalanine, serine and threonine on cellulose with butanol - acetic acid - water 11:4:5 in a saturated chamber. Detection by spraying with ninhydrin reagent, followed by heating on a plate heater. The hRf values of the amino acids were 30, 36, 44, 50, 52, 72, and 79, respectively.

J. Planar Chromatogr. 28, 248-250 (2015). TLC of phenylalanine, tyrosine and tryptophan on silica gel prepared by adding different concentrations of vancomycin with n-butanol - methanol - water - acetic acid 25:8:6:2. The best separations of the three racemic amino acids were achieved with more than 1.86 g of vancomycin in 25 g of silica gel. Vancomycin proved to be sufficiently stable and showed good enantioselectivity.

Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 67, (1984). OPLC (OPTLC) of amino acids on silica with 1) butanol - acetic acid - water 4:1:1, 2) phenol -1 % SDS - isobutanol - propanol - butanol - acetic acid 70:28:2:2:2:1.7. Detection with ninhydrin + Cu or Cd acetate reagent. Detection with videodensitometer, coefficient of variation 6 %.

J. Chromatogr. 333, 69-81 (1985). Study on the influence of temperature gradient for different chromatographic systems using the tank developing configuration. Illustration of the method by chromatography of dansyl amino acids and aflatoxins. The enantiomers of the dansyl amino acids could be separated by 2-D-RP TLC with partial separation, then after spraying with Cu complex of N,N-di-n-propyl-L-alanine by separation in a temperature gradient. Aflatoxins B1, B2, G1, G2 were separated on silica with a temperature gradient of 20/min. over 150-180 min.

Hungarian Scientific Instruments 62, 1-9 (1986). Separation of amino acids and S-containing amino acids on cellulose with MEK -acetonitrile - acetic acid - water 3:4:1:2. Separation of N-methylated and N-formylated L-lysine on silica with propanol - methanol - 0.1 M aqueous Na-acetate 20:3:30, Separation of ether soluble DN amino acids on silica with chloroform - carbon tetrachloride -MEK - propanol - methanol - acetic acid 30:30:20:30:15:2. Separation of corticosteroids with acetonitrile - butyl acetate 1:1 and of trigonelline from tomato juice with propanol - methanol - 0.1 M Na-acetate. Appropriate staining reagents; densitometry by absorbance.

Chromatographia 23, 289-291 (1987). TLC of fluorescent-labelled cysteine and its homologue homocysteine with monobromo-bimane on silica with 4 different solvent systems. Identification by their intense yellow fluorescence under UV at 366 nm. Detection limit 2 ng for both.

J. Chromatogr. 393, 407-417 (1987). Two- dimensional TLC of 22 dansylated amino acids on micropolyamide based on a previously described procedure. Quantification by autoradiography. Detection limit, 10 to the minus 13 mol with specific activity of less than 5 GBq/mmol.

Chromatographia 24, 725-738 (1987). TLC on chiral-plates with four solvent systems. Detection by spraying with 0. 1% ninhydrin in ethanol. Comparison of Rf values and resolution factors.