Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 006
      Application of Taguchi OA and Box–Behnken design for the implementation of DoE-based AQbD approach to HPTLC method for simultaneous estimation of azilsartan and cilnidipine
      P. PRAJAPATI*, P. TAILOR, A. SHAHI, A. ACHARYA, S. SHAH
      (*Department of Quality Assurance, Maliba Pharmacy College, Uka Tarsadia University, Tarsadi, Mahuva, Surat, Gujarat, India; pintu21083@gmail.com)

      J Chrom Sci, bmad045 (2022). Standards were azilsartan medoxomil (AZL) and cilnidipine (CLN). Samples were acetonitrile solutions of commercial tablets of AZL and CLN, and purified human blood plasma as biological fluid spiked with AZL and CLN. The following method was developed by a software-assisted AQbD approach (analytical quality by design): (1) Taguchi orthogonal array design was implemented in 8 screening experiments in order to identify the 3 critical method variables (CMVs), which were: volume ratio of toluene – ethyl acetate, volume of methanol and saturation time. These CMVs had statistically significant impact (one-way ANOVA and Pareto charts) on the 3 critical analytical attributes (CAAs, they were: resolution between AZL and CLN and their hRF values). (2) To optimize these CMVs, the Box–Behnken design was implemented in 15 software-proposed experiments; the impacts of the 3 CMVs on the 3 CAAs were evaluated by ANOVA, multiple regression analysis, and 2D and 3D contour plots; the response surface analysis allowed the software to find a mathematical (quadratic or linear) equation for each CAA, based on the CMVs values. (3) The optimal CMVs ranges were determined by defining an analytical design space (ADS) on the superposed contour plots, and one TLC condition was selected as analytical control point.
      TLC on silica gel pre-washed with 10 mL methanol, dried and activated 15 min at 110° C. Separation with toluene – ethyl acetate – methanol 13:3:4 after 15 min pre-saturation with 35 % relative humidity. Absorption measurement at UV 254 nm. The hRF values were 49–51 for AZL and 70–71 for LRT. Linearity range was 400–2000 ng/zone for AZL and 100–500 ng/zone for CLN. Intermediate precision was below 1.6 % (n=3). LOQ were 121 ng/zone for AZL and 34 ng/zone for CLN. Recovery rates were 99.3–99.7 % for AZL and 98.1–99.5 % for CLN. Recovery rates from spiked plasma were 83.3 % for both molecules.

      Classification: 2e, 7, 8b, 16, 23d, 23e, 32a
      129 060
      Detection of low levels of genotoxic compounds in food contact materials using an alternative HPTLC-SOS-Umu-C assay
      D. MEYER, M. MARIN-KUAN, E. DEBON, P. SERRANT, C. COTTET-FONTANNAZ, B. SCHILTER, Gertrud E. MORLOCK*
      (*Institute of Nutritional Science, Justus Liebig University Giessen, and TransMIT Center of Effect-Directed Analysis, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      ALTEX - Alternatives to animal experimentation, 38(3), 387-397 (2021). Samples were standards of food contact contaminants with genotoxicity (4-nitroquinoline-1-oxide (NQO), aflatoxin B1, hexachloroethane, nitroso-ethylurea, phenformin, PhIP) or negative controls (alosetron, mannitol), and extracts of coated tin cans (extracted with n-hexane – acetone at 25°C for 16 h or by heating at 60 °C with ethanol 95 % for 240 h). HPTLC on RP18W layer, pretreated to harden the binder by heating 1 h at 120 °C, prewashed with methanol and with ethyl acetate and dried 4 min in cold air stream after each development. Application areas were focused to their upper edges by a two-fold elution with ethyl acetate, followed by 1 min drying in cold air stream. Development with toluene – ethyl acetate 8:5, followed by 5 min drying, neutralization with citrate buffer (pH 12) and 4 min drying. Effect-directed analysis for genotoxicity (SOS response – UMU-C test, using NQO as positive control) by immersion (speed 3.5 cm/s, time 3 s) into Salmonella typhimurium suspension and, after 3 h incubation at 37 °C and 4 min drying in cold air stream, into one of two fluorogenic substrate solutions (methylumbelliferyl- vs. resorufin-galactopyranoside). After 1 h incubation at 37 °C, visualization of mutagenic compounds as (blue vs. red) fluorescent zones at FLD 366 nm, and densitometry performed with mercury lamp for fluorescence (at  366 / >400nm vs. 550 / >580 nm, respectively). Further validation experiments, including spiking extracts with NQO, were performed showing good mean reproducibility, no quenching or other matrix effects. Lowest effective concentration of NQO was 0.53 nM (20 pg/band), 176 times lower than in the corresponding microtiter plate assays.

      Classification: 4e, 5c, 8b, 16, 23d, 23e, 32d
      59 085
      Two-dimensional thin-layer chromatography for the separation and identification of nitro derivatives in explosives
      L. BAGNATO*, G. GRASSO, (*Ministero Difensa, Direzione di Artiglieria, Corso Malta 102, I-80143 Naples)

      J. Chromatogr. 357, 440-444 (1986). Two-dimensional TLC of aliphatic and heterocyclic nitro derivatives on silica with petrol ether - dichloromethane, petrol ether - acetone, petrol ether - ethyl acetate, chloroform - acetone or petrol ether - benzene - acetone in various combinations. Detection by spraying with 1 % diphenylamine in ethanol - acetone 1:1.

      Classification: 16
      91 038
      Determination of N-nitrosamines by HPTLC with fluorescence detection - Use of non-ionic surfactants as enhancing agents
      L. CARDENES, J.H. AYALA, V. GONZALES, A.M. AFONSO*, (*Dept. of Anal. Chem., Nutrition and Food Sci., Univ. of La Laguna, Campus de Anchieta, Astrofisico Francisco Sanchez s/n, E-38205 La Laguna, Spain)

      J. Planar Chromatogr. 15, 349-353 (2002). HPTLC of the dansyl derivatives of N-nitrosodimethylamine, N-nitrosodiethylamine, and N-nitrosodibutylamine on silica gel, protected from light, with hexane - ether - dichloromethane 10:3:2. A fiber-optic-based fluorescence instrument for in-situ scanning was used for quantitative measurements at 317 and 521 nm. Dipping the plates in a mixture of the surfactant polyoxyethylene-10-lauryl ether enhanced both the fluorescence intensity and the signal-to-noise ratio of the dansyl derivatives. The detection limits ranging between 0.47 and 0.89 ng were five times lower than those obtained in the absence of the surfactant.

      Keywords:
      Classification: 16
      60 103
      Thin-layer chromatography of isosorbide dinitrate, nitroglycerin and their degradation products
      M. CARLSON*, R. THOMPSON, (*U.S. Food & Drug Adm., Dept. Health & Human Services, 240 Hennepin Avenue, Minneapolis, MN 55401, USA)

      J. Chromatogr. 368, 472-475 (1986). TLC on silica with toluene - ethyl acetate 1:1. Detection by spraying with 0.1 % sodiummetaperiodate and 0.5 % potassium permanganate in 4 % solution of sodium hydroxide and heating at 100 °C for 1-5 min. The reported procedure is also useful for the isolation of other related organic nitrate esters including erythrityl tetranitrate, pentaerythritol tetranitrate and mannitol hexanitrate. Isosorbide dinitrate, nitroglycerin.

      Keywords:
      Classification: 16
      62 009
      Lipohilicity determination of some nitrostyrene derivatives on RP-2, RP-8 and RP-18 layers
      T. CSERHATI, A. KISS-TAMAS, GY. MIKITE, (Plant Protec. Inst., Hungarian Acad. Sci., Herman O. 15, H-1022 Budapest, Hungary)

      Chromatographia 25, 82-86 (1988). Determination of the lipophilicity of 33 nitrostyrene derivatives by reversed-phase TLC on three alkyl- bonded silicas with methanol. Discussion of the relationship between RM value of each compound and the concentration of the organic solvent, the retention strength and selec tivities of the three bonded phases, and the impact of the change of methanol concentration on the retention.

      Keywords:
      Classification: 2a, 16
      63 087
      Robust derivative for the determination of 1-nitropyrene by fluorescence scanning densitometry
      T.A. DEAN, C.F. POOLE, (Dep. Chem., Wayne State Univ., Detroit, MI 48202 USA)

      J. Planar Chromatogr. 1, 70-72 (1988). TLC of the N, N’-dimethylamino derivative of 1-nitropyrene on silica and octadecyl-bonded silica. Quantification by fluorodensitometry. Detection limit, ˜ 10pg. Comparison of the fluorescence intensity and the stability of the N, N’-dimethylamino and the amino derivatives of the compound.

      Classification: 16
      65 093
      Trifluoroacetic anhydride-sodium iodide as a reagent for the selective detection of nitrones and nitroxide radicals by thin-layer chromatography
      J. DRABOWICZ, A. KOTYNSKI, Z.H. KUDZIN*, R. SKOWRONSKI, (Inst. Chem., Univ. Lodz, Narutowicza 68, 90-136 Lodz, Poland)

      J. Chromatogr. 473, 287-292 (1989). Presentation of a selective procedure for the detection of nitrones and nitroxide radicals in the presence of amine N-oxides on the TLC plate based on the application of trifluoroacetic anhydride-sodium iodide reagent. Discussion of possible interferences and their elimination.

      Keywords:
      Classification: 3e, 16