J. Planar. Chromatogr. 28, 173-177 (2015). HPTLC of (1) sclareol, (2) linalool, (3) linalyl acetate, and (4) carvone on silica gel with n-hexane - ethyl acetate 17:3. Detection at UV 254 nm and in daylight after dipping in vanillin - sulfuric acid followed by heating at 110 °C for 5 min. The presence of (1) in Clary Sage essential oil was demonstrated by 2D on silica gel with toluene - ethanol 17:3 in the first direction and n-hexane - acetone in the second direction, and on RP-18 phases with acetonitrile - water 7:3. Bioassays were performed by the direct bioautographic system using a Gram-negative test bacteria (Xanthomanas euvesicatoria), the luminescence gene-tagged Arabidopsis pathogen (Pseudomonas syringae pv. maculicola) and the luminescent marine bacterium (Aliivibrio fischeri) as well as a Gram-positive soil bacterium (Bacillus subtilis). For Xanthomanas euvesicatoria and Bacillus subtilis, the dried layers were dipped into cell suspension culture and after 2 h incubation in a vapor chamber at 28 °C, they were immersed into an aqueous MTT solution. The bright zones against the darker background indicate the presence of antibacterial components. For luminescent bacterial strains, the plate was dipped into bacteria suspension and evaluated with a computer-controlled cooled low-light camera. Images of bioautograms were directly recorded with an exposition time of 5 min for Aliivibrio fischeri and 15 min for Pseudomonas syringae pv. maculicola. The dark spots lacking luminescence indicate the antibacterial activity. Sclareol inhibited all tested bacteria, linalool and carvone showed antibacterial effect against all Gram-negative strains tested, while linalyl acetate only against Xanthomanas euvesicatoria and Aliivibrio fischeri.

Chinese J.Pharm.Anal. 8, 33-36 (1988) (Yaowu Fenxi Zazhi). TLC of d-menthone and 1-pulegone on alkali-modified silica with petrol ether - ethyl acetate 9:1. Detection by spraying with vanillin - sulfuric acid - ethanol 1:1:18 and heating at 80 °C for 20 min.

Olaj, Szappan, Kozmetika 38, 28-32 (1989). TLC of perfume oils on silica with benzene - ethyl acetate 8:2, dichloromethane - petrol ether 7:3 and toluene - ethyl acetate 98:2. Visualization under UV and by spraying with 3% sulfuric acid - vanillin reagent followed by heating at 105°C for 5 min.

J. Chromatogr. 624, 497-502 (1992). TLC of title compounds on silica with butanol - acetic acid - water 4:1:1. Detection of crocin and crocetins in white light, and of picrocrocin under UV 254 nm. TLC of safranal on silica with hexane - ethyl acetate 9:1; detection by spraying with 2,4-dinitrophenylhydrazine reagent. Quantification by spectrophotometry. Also HPLC and GC method. Comparison of the three methods.

J. Liq. Chromatogr. Relat. Technol. 28, 2525-2537 (2005). TLC of 15 selected essential oil components, alcohols (geraniol, linalool, menthol, (+)-borneol), phenols (vanillin, eugenol, guaiacol, thymol), ether ketones, and aldehydes (cineole, trans-anethole, (R)-(-)-carvone, (R)-(-)-fenchone, coumarin, camphor, cinnamic aldehyde) on silica gel and alumina with 26 mobile phases after saturation for 30 min. Aluminium oxide plates and carbon tetrachloride - acetone 49:1 were best suited for the separation of the investigated alcohols; aluminium oxide plates and carbon tetrachloride - acetone 17:3 for the separation of the investigated phenols, and alumina with chlorobenzene - acetone (19:1 for the separation of coumarin, cineole, carvone, cinnamic aldehyde, campher, fenchone, and trans-anethole. Detection by spraying with a 5 % solution of potassium dichromate in 40 % sulfuric acid.

J. Liq. Chromatogr. Relat. Technol. 38, 1794-1801 (2015). 2D-TLC of eleven essential oils from nine Mentha species on silica gel with ethyl acetate - toluene 5:19 in the first direction and ethyl acetate - n-heptane 3:17 in the second direction. Detection by dipping into p-anisaldehyde reagent (0.5 mL p-anisaldehyde solution in 50 mL glacial acetic acid and 1 mL concentrated sulfuric acid) followed by heating at 105 °C for 10 min. Evaluation under 254 nm. The developed plates were immersed in a 0.2 % methanolic solution of 2,2-diphenyl-1-picrylhydrazyl (DPPH radical reagent) and scanned after 30 min.

Spongian diterpenes from Hyatella intestinalis. J. of Natural Products 51, 293-297 (1988). TLC of 3ß,19-diacetoxyspongia-13(16),14-dien-2-one and 19-hydroxyspongia-13(16),14-dien-3-one on silica with ethyl acetate - hexane 1:5 or 1:10 acetone - hexane 1:1. Preparative TLC of 2a,19-dihydroxyspongia-13(16),14-dien-3-one on silica with benzene - ether 2:1.

part.) (Hungarian). Gyogyszereszet 33, 197-201 (1989). TLC of pinene, sclareol, farnesol, borneol, geraniol, linalool on silica with chloroform - dioxane 9:1, benzene - ethyl acetate 8:2 and benzene - dioxane - chloroform 45:10:45. Visualization under UV and by spraying with vanillin - sulfuric acid reagent.