Marine Drugs 21(1), 2 (2023). Samples were methanol extracts of cultivated marine bacteria Thalassomonas actiniarum, T. viridans and T. haliotis (Colwelliaceae),  as well as cholesterol, cholic acid, and deoxycholic acid as standards. TLC on silica gel with n-hexane – ethyl acetate – methanol – acetic acid 20:20:5:2. After drying at room temperature, visualization by spraying with phosphomolybdic acid (10 % in ethanol) and heating with a heat-gun. For isolation of cholic acid (hRF 80), present in all samples, preparative TLC on silica gel with the same mobile phase, the corresponding band was scraped off with a surgical blade and extracted with methanol overnight. The isolated cholic acid was identified by LC-MS.

J. Planar Chromatogr. 33, 271-279 (2020). Two-dimensional HPTLC of cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic, and lithocholic bile acids on silica gel with 0.0001 M cetylpyridinium chloride at pH 9 with the addition of aliphatic alcohol modifiers 4 % 1-butanol (direction II) and 0.6 % 1-pentanol (direction II). Detection by drying the plate for 2-3 min in the oven at 120 ºC, followed by spraying with 8 % sulfuric acid in ethanol. Qualitative determination under UV light at 365 nm.

J. AOAC Int. 102, 708-713 (2019). TLC of snake bile in 20 species from three families (Elapidae, Colubridae, and Viperidae) on silica gel with xylene – isopropanol – methanol – glacial acetic acid – water 80:40:30:20:3. Detection by spraying with a 10% sulfuric acid ethanol solution, followed by heating at 105 ºC.  Qualitative identification under UV light at 366 nm. TLC coupled with quadrupole–time-of-flight–MS analysis of each zone was used for compound identification and evaluation.

Proc. Euro Food Pack, Intern. Conference on food packing, Vienna, 275-279 (1984). Two-dimensional TLC of 5-cholestene 3 beta,7 alpha-diol; 3,5-cholestadiene-7-one; cholestan-3 beta, 5 alpha, 6 beta-triol on silica with 1) chloroform - acetone 9:1, 2) chloroform - ether 1:1. Detection by UV. TLC study oxidation of cholesterol in heated tallow, photooxidation of cholesterol in butter.

Talanta 37, 471-480 (1990). TLC of several bile acids and bile salts on silica with isooctane – ethyl acetate – acetic acid 10:10:2 and butanol – acetic acid – water 4:1:1. Identification by liquid-state secondary-ion MS. Quantification by monitoring the ion intensity for the sample spot and the standard spot developed in parallel.

J. Liq. Chromatogr. Relat. Technol. 28, 297-311 (2005). TLC of cholic acid, glycocholic acid, glycodeoxycholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, and glycolithocholic acid on RP-18 W with methanol - water, (acetonitrile - methanol 1:1) - water, acetone - water, dioxane - water in different volume compositions. Detection by spraying with 10 % aqueous solution of sulfuric acid or by dipping in 10 % ethanolic solution of phosphomolybdic acid followed by heating at 120 °C for 20 min.

behavior of some steroids by OPLC. Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 90 (1984). OPLC (OPTLC) of progesterone, cholesterol, corticosterone, aldosterone, cortisol, cortisone on silica with a) petrol ether - ethyl-acetate 6:4, b) chloroform - ethyl acetate - ethanol 90:10:1, c) chloroform - phenol 9:1. Detection with 50 % phosphoric acid and heating at 105 °C for 15 minutes.

Chinese J. Hosp. Pharm. (Zhongquo Yiyuan Yaoxue Zazhi) 11, 260-262 (1991). TLC of taurocholic acid and hyodeoxycholic acid on silica with cyclohexane – ethyl acetate – acetic acid 4:4:1. Quantification by densitometry at 400 nm.