Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 95:5. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated: isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.

J. Liquid Chrom. 10, 1277-1290 (1987). TLC on silica, Ag+ impregnated silica with e.g. isopropyl ether - acetic acid 96:4, then with petrol ether - acetic acid 90:10:1. Also chromatography on RP-18 silica. Derivatization with phosphomolybdic acid reagent, for quantification with copper acetate - H3PO4 reagent. Quantification by densitometry.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 49-55 (1991). Separation of ceramides and cholesterol on silica, using a four-step 2-D developing technique: first direction with chloroform - acetone - methanol 90:5:5 over 1 cm, second direction with the same solvent over 8.5 cm, then in the first direction again with chloroform - acetone - methanol - NH3 60:30:8:2, and finally with chloroform - acetone - methanol - acetic acid - water 78:16:4:2:1 in the second direction. Derivatization by dipping in phosphoric acid - acetic acid - sulfuric acid - water 10:10:1:180 followed by heating at 200 °C for 10 min. Method for routine analysis of ceramides.

Chinese J. Herb Med. (Zhongcaoyao) 24, 128-130 (1993). TLC of palmityl-ß-sitosterol, lupenone, 24-methylene-cycloartenol, and ß-sitosterol on silica with chloroform - benzene 9:1. Detection by spraying with 5% phosphomolybdic acid. Quantification by densitometry (absorbance) at 625 nm.

J. Planar Chromatogr. 13, 437-442 (2000). TLC of cholesterol and oxysterols (7-ketosterol, 25-hydroxycholesterol, 7b-hydroxycholesterol, cholesterol-5a,6a-epoxide, cholestane-3b,5a,6b-triol) on silica gel with chloroform-acetone 9:1. After spraying with Liebermann-Burchard reagent the spots were quantified by densitometry in reflectance mode at 360 nm. Also TLC of silylated oxysterols on silanized silica gel with n-hexane - ether - methanol 38:2:1. Simple, sensitive, and reproducible method.

Chromatographia 67 (3-4), 268-274 (2008). HPTLC of 24beta-ethylcholesta-5,22E,25-triene-3beta-ol (ECTO) in the aerial part of Clerodendrum phlomidis (used as a chemical marker for the standardization of C. phlomidis plant extracts) on silica gel with chloroform - methanol 197:3. Detection by spraying with anisaldehyde reagent. Quantitative determination by densitometry in absorption mode at 650 nm. Linearity was between 150 and 400 ng/band with good correlation (r2 = 0.996).

J. Planar Chromatogr. 30, 193-198 (2017). HPTLC of 20-hydroxyecdysone in Sesuvium portulacastrum on silica gel with chloroform – methanol – benzene 25:5:3. Quantitative determination by absorbance measurement at 254 nm. The hRF value for 20-hydroxyecdysone was 30. Linearity was between 50 and 500 ng/zone. LOD and LOQ were 4 and 14 ng/zone. The intermediate precision was below 2 % (n=6). The recovery rate ranged from 98.6 to 102.4 %.

Phytochemistry 23, 2925-2929 (1984). Isolation of sterols from a crude extract of cucurbita maxima seeds by PLC on silica with ether - benzene 1:9 (4 developments). Detection by UV after spraying with 0.05 % solution of rhodamine 6G in acetone. Method suitable for the separation of delta 5-sterols from delta 7-sterols. See also: V. Garg and W. Nes, Phytochemistry 23, 2919 (1984).