Anal. Biochem. 239, 115-117 (1996). TLC of sterols on silica gel with hexane - ethyl ether - acetic acid 80:15:1. Dried plates were rehydrated for 30 sec in PBS and then incubated for 30 min in filipin suspension at 37°C in the dark. Afterwards washed 2 x 2 min in water. Spots visualized at 365 nm. Detection limit 5 ng cholesterol.

J. Chromatogr. A 1026 (1-2), 301-304 (2004) A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100 % depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected by TLC. Detection by fluorescence measurement, quantification limit was 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

Food Chem. 221, 1232-1244 (2017). Review of novel analytical methods for the detection of milk adulterants, including the use of RP-TLC for the determination of vegetable oils as adulterant of fat content. In this methodology, vegetable oil content was quantified by monitoring the structure of sterols by using β-sitosterol as a marker.

J. Planar Chromatogr. 31, 332-336 (2018). HPTLC of β-sitosterol (1) and lupeol (2) in Saussurea lappa and Saussurea auriculata on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid 29:9:2. Detection by spraying with anisaldehyde–sulfuric acid reagent. Quantitative determination by absorbance measurement at 525 nm. The hRf values for (1) and (2) were 71 and 88, respectively. Linearity was between 100 and 400 ng/zone. LOD and LOQ were 7 and 23 ng for (1), and 6 and 17 ng for (2), respectively. The intermediate precision was <2 %. Average recovery was 99.9 % for (1) and 100.0 % for (2).

J. A.O.A.C. 70, 499-501 (1987). TLC determination of coprostanol and cholesterol on silica, impregnated with 5 % alcoholic phosphomolybdic acid solution, with ether - heptane 55:45. Detection by heating for 20 min at 120 °C.

J. Liquid Chromatogr. 12, 3151-3161 (1989). TLC of sterols and other lipids on silica with isopropyl ether – acetic acid 96:4, followed by hexane – ether – acetic acid 90:10:1. Detection of lipids by spraying with 5% ethanolic phosphomolybdic acid and heating for 5-10 min at 100-120°C. Semiquantification by comparison of the zone sizes and intensities of standards and samples. Quantification of cholesterol by densitometry after development with chloroform – ethyl acetate 96:4, and dipping in cupric acetate – phosphoric acid reagent and heating at 100°C. Quantification of triacylglycerols and free fatty acids by densitometry after development with hexane – ether – acetic acid 80:20:1, followed by spraying with cupric acetate – phosphoric acid reagent. Also GC.

Anal. Chim. Acta 275, 177-182 (1993). HPTLC of corticosteroids on silica with chloroform - methanol 98:2. Detection by spraying with a mixed solution of 1% sulfuric acid in acetic acid, and heating for 10 min. at 95 °C. Examination in daylight and under UV 366 nm.

J. Liqu. Chromatogr. 23, 282-294 (2000). Comparison of techniques TLC of 20-hydroxyecdysone and polypodine B (5,20-dihydroxyecdysone) on silica gel with dichloromethane - ethanol (96%) 4:1, chloroform - methanol - benzene 25:5:2, and ethyl acetate - methanol - conc. NH3 17:2:1. Detection directly after development under UV 254 nm; quantitation by densitometry at 254 nm in reflectance mode. - Parallel analyses using both TLC with densitometry and HPLC gave similar results.