(TLC chromatographic analysis for the quantitative estimation of the fecal bile acid composition). TLC of cholic acid, chenodesoxy cholic acid, desoxy cholic acid, lithocholic acid and ursodesoxy cholic acid on silica with isooctane - isopropanol - acetic acid 30:10:1 and/or isooctane - ethyl acetate - acetic acid 10:10:2. Derivatization with 2 % ethanolic solution of 2,7-dichlorofluoresceine. Densitometric quantification by fluorescence. Fiber rich polysaccharides (dietary fibers) elevate fecal bile acids and their metabolites. The possible role of this diet in relation to fecal bile acid concentration and large bowel cancer is discussed.

J.Trad.Chin.Med (Zhongguo Zhongyao Zazhi) 14, 466-468 (1989). TLC of D7- stigmastenol and fructose on silica with cyclohexane - ethyl acetate 4:1, and benzene - acetic acid - methanol 15:6:4. Quantification by densitometry (absorbance) at 540 nm and 500 nm.

Anal. Chim. Acta 275, 147-162 (1993). HPTLC of the anabolic residues in biological samples on silica with seven solvent systems in one or in two dimensions. Detection under UV 366 nm. Also, HPLC and GC/MS. Discussion of the influence of the matrix and the applied methods on the results.

Anal. Biochem. 239, 115-117 (1996). TLC of sterols on silica gel with hexane - ethyl ether - acetic acid 80:15:1. Dried plates were rehydrated for 30 sec in PBS and then incubated for 30 min in filipin suspension at 37°C in the dark. Afterwards washed 2 x 2 min in water. Spots visualized at 365 nm. Detection limit 5 ng cholesterol.

J. Chromatogr. A 1026 (1-2), 301-304 (2004) A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100 % depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected by TLC. Detection by fluorescence measurement, quantification limit was 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

Food Chem. 221, 1232-1244 (2017). Review of novel analytical methods for the detection of milk adulterants, including the use of RP-TLC for the determination of vegetable oils as adulterant of fat content. In this methodology, vegetable oil content was quantified by monitoring the structure of sterols by using β-sitosterol as a marker.

J. Planar Chromatogr. 31, 332-336 (2018). HPTLC of β-sitosterol (1) and lupeol (2) in Saussurea lappa and Saussurea auriculata on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid 29:9:2. Detection by spraying with anisaldehyde–sulfuric acid reagent. Quantitative determination by absorbance measurement at 525 nm. The hRf values for (1) and (2) were 71 and 88, respectively. Linearity was between 100 and 400 ng/zone. LOD and LOQ were 7 and 23 ng for (1), and 6 and 17 ng for (2), respectively. The intermediate precision was <2 %. Average recovery was 99.9 % for (1) and 100.0 % for (2).

J. A.O.A.C. 70, 499-501 (1987). TLC determination of coprostanol and cholesterol on silica, impregnated with 5 % alcoholic phosphomolybdic acid solution, with ether - heptane 55:45. Detection by heating for 20 min at 120 °C.