J. Planar Chromatogr. 35, 501-507 (2022). HPTLC of β-sitosterol the seeds of Syzygium cumini on silica gel with toluene - ethyl acetate - methanol - glacial acetic acid 6:3:2:1. Detection by dipping into anisaldehyde sulfuric acid solution for 3 s, followed by heating at 80-100 °C for 2-3 min. The assessment of α-amylase inhibitors was performed by dipping into enzyme solution (10 mg α-amylase enzyme in 20 mL of sodium acetate buffer and stored at 2–8 °C) for 2–3 seconds, followed by 90 min humidification in a desiccator and the dipping into a 1 % starch solution as a substrate and put in a humid environment for additional 20–30 min to allow the enzyme–substrate interaction to occur. After a 2–5-min time, the plate was washed or dipped in Gram’s iodine blue, which revealed anti-diabetic activity as blue stains on a white background. The hRF value for β-sitosterol was 87. Further analysis by high‑resolution mass spectrometry.

J. Planar Chromatogr. 35, 403-410 (2022). HPTLC of β-sitosterol (1), ferulic acid (2), berberrubine (3), griffonilide (4) and lithospermoside (5) in Semiaquilegia adoxoides on silica gel with cyclohexane - ethyl acetate 3:1 for (1), toluene - ethyl acetate - methanol - formic acid 50:40:5:12 for (1) and (2), n-butanol - acetic acid - water 21:3:6 for (3) and  chloroform - methanol - water 12:6:1 for (3) to (5). Detection of (1) and (2) by spraying with 10 % sulfuric acid in ethanol, followed by heating at 105 °C. Analysis under UV light at 254 and 366 nm.

J. Planar Chromatogr. 35, 13-21 (2022). HPTLC of guggulsterone E (1) and Z (2) in Commiphora mukul on silica gel with toluene - acetone 9:1. Quantitative determination by absorbance measurement at 250 nm. The hRF values for (1) and (2) were 18 and 23, respectively. Linearity was between 60 and 360 ng/zone for (1) and (2). 

Nature - Lab. Invest. 100, 1411–1424 (2020). Samples were chloroform – methanol 1:1 solutions of lipid standards and of liver tissue extracts from wild-type mice (1), from transgenic murine models of hepatic steatosis (2) (mice expressing HBs, hepatitis B virus surface protein), or of cholestasis (3) (mice totally knock-out for the gene of phospholipid translocator ABCB4, ATP-binding cassette subfamily B member 4), or of both (4) (hybrids of mice (2) and (3)). HPTLC on silica gel (preheated at 110°C for 15 min) with n-hexane – diethyl ether – acetic acid 20:5:1. (A) For qualitative analysis, visualization under white light after immersion into anisaldehyde 0.5 % (in sulfuric acid – acetic acid – methanol, 1:2:17), followed by heating at 110°C for 9 min. (B) Identification of lipids was confirmed by elution of the zones of interest with methanol from the HPTLC layer through a TLC-MS interface and a filter frit directly to a quadrupole-orbitrap MS (atmospheric pressure chemical ionization, full HR-MS scan in m/z range 100–1000). (C) For quantitative analysis, visualization at UV 366 nm after derivatization by immersion into primuline reagent (primuline 0.5 g/L in acetone – water 4:1); fluorescence was measured at UV 366 nm (mercury lamp, optical filter for wavelengths above 400 nm, scanning slit 6.0 mm × 0.2 mm, speed 20 mm/s). (A) and (B) allowed the separation and detection of cholesterol, cholesteryl oleate, methyl oleate, free fatty acids (FFA, expressed as oleic acid equivalents) and triacylglycerols (TAG, as triolein equivalents) in liver extracts. (C) showed that TAG was decreased and FFA increased in (3) and (4), compared to (1) and (2). Cholesterol and cholesteryl oleate had no significant changes between groups.

J. Planar Chromatogr. 34, 173-181 (2021). HPTLC of caffeic acid (1), chlorogenic acid (2), quercetin (3), oleanolic acid (4), lupeol (5), betulinic acid (6), β-Sitosterol (7), campesterol (8) and ergosterol (9) in samples of Echinops echinatus on silica gel with toluene - ethyl acetate - formic acid - methanol 30:30:8:3 for (1) and (2), toluene - ethyl acetate - formic acid 5:4:1 for (3), toluene - methanol 9:1 for (4), petroleum ether - ethyl acetate - toluene 7:2:1 for (5) and (6), toluene - ethyl acetate 9:1 for (7) and toluene - methanol - formic acid for (8) and (9). Quantitative determination by absorbance measurement at 254 nm for (1) to (3), 540 nm for (4) to (6) and 530 nm for (7) to (9). The hRF values for (1) to (9) were 69, 80, 60, 30, 68, 55, 26, 67 and 75, respectively. Linearity was between 2 and 12 µg/mL for (1) to (9). Intermediate precision was below 2 % (n=6). The LOD and LOQ were 38 and 119 ng/zone for (1), 18 and 57 ng/zone for (2), 364 and 1100 ng/zone for (3), 11 and 32 ng/zone for (4), 40 and 123 ng/zone for (5), 15 and 46 ng/zone for (6), 8 and 23 ng/zone for (7), 52 and 159 ng/zone for (8) and 527 and 1598 ng/zone for (9), respectively. Recovery was between 95.7 and 99.6 % for (1) to (9). 

Planta Med. 85(3), 195-202 (2019). The dichloromethane fraction of an ethanolic extract from Gloeophyllum odoratum sporocarp (Gloeophyllaceae, Basidiomycetes) was submitted to a multistep purification process (conventional, flash and supercritical fluid column chromatography). At each step, fractions were monitored on TLC silica gel with dichloromethane – methanol – water 40:4:1. Detection under white and UV light after derivatization with vanillin sulfuric acid 5 % in methanol and heating. Eight triterpenes were isolated for further identification: eburicodiol, gloeophyllins B and K, hydroxylanosterol, trametenolic acid B (all five from the lanostane type), gloeophyllins A and L (C‑nor-D-homoergosteroid type), and ergosterol peroxide (ergostane type).

J. Planar Chromatogr. 32, 243-249 (2019). HPTLC of stigmasterol (1) and cinnamic acid (2) in Pluchea dioscoridis on silica gel with chloroform - methanol - acetic acid 93:5:2. Quantitative determination of (1) by absorbance measurement at 254 nm. Compound (2) was detected by spraying with p-anisaldehyde and quantified under UV light at 513 nm. The hRF values for (1) and (2) were 57 and 19, respectivley. Linearity was between 200 and 1400 ng/zone for (1) and (2). The intermediate precision was below 2 % (n=6). The LOD and LOQ were 39 and 117 ng for (1) and 43 and 129 ng for (2), respectively. Recovery rate was between 98.8 and 99.4 % for (1) and 98.4 and 99.0 % for (2).

J. Planar Chromatogr. 27, 157-161 (2014). HPTLC of withanoside V (1), withaferine A (2), 1,2-deoxywithastramonolide (3), withanone (4), withanolide A (5), and withanolide B (6) on silica gel with dichloromethane - toluene - methanol - acetone - diethyl ether 13:14:8:3:1. Quantitative determination by absorbance measurement at 235 nm. The hRF values for (1) to (6) were 7, 55, 61, 63, 66 and 77, respectively. Precision was below 2 % (n=3). The LOD and LOQ were 60 and 250 ng/zone for (1), 120 and 350 ng/zone for (2), 80 and 300 ng/zone for (3), (4) and (6), and 60 and 250 ng/zone for (5), respectively. Average recoveries for (1) to (6) were 97, 98, 99, 98, 98 and 99 %, respectively.