Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      120 060
      Two-dimensional thin-layer chromatography of phytoestrogens on RP-18 W plate, detected by effect-directed analysis using the yeast estrogen screen test
      Y. MINAR, B. SPANGENBERG* (*University of Offenburg, Department of Process Engineering, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 30, 423-426 (2017). 2D-HPTLC of phytoestrogenic active compounds in the root of Glycyrrhiza glabra on RP-18 with hexane – ethyl acetate – acetone 9:3:2 in the first direction and acetone – water 3:2 in the second direction. Effect-direct analysis by dipping into a yeast suspension followed by incubation at 30 °C for 4 h, drying at 37 °C for 15 min and spraying with the combined reaction buffer C (20 mL reaction buffer C is mixed with 0.2 mL of a freshly prepared solution of 0.05 g/mL 4-methylumbelliferyl-ß-D-galactopyranoside in DMSO or 0.2 mL of a freshly prepared solution of 0.05 g/mL 5-bromo-4-chloro-3-indoxyl-ß-D-galactopyranoside (X-Gal) in DMSO). Fluorescence detection at UV 366 nm._x000D_

      Classification: 13b
      85 057
      Chromatographic methods in pharmaceutical purity testing
      K. FERENCZI-FODOR, B. BAGOCSI, D. FABIAN, E. GÜRTLER, Z. VEGH, (Gedeon Richter Ltd., P.O.Box 27, 1475 Budapest 10, Hungary)

      Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 19-25. OPLC of ethinyl estradiole on silica gel with cyclohexane - ethyl acetate - chloroform 3:1:1. Detection by spraying with sulfuric acid and visual evaluation at 366 nm. The method is compared with the Official British and European Pharmacopoeia monographs. Only with the OPLC method it was possible to separate all impurities, i.e. 6-hydroxy derivatives (both isomers), the oxo-derivatives (6-oxo- and 16-oxo-), the 9(11)-dehydro and the 17-epi-derivatives from the estrone and estradiol. The HPLC method of the 3rd European Pharmacopoeia has to be changed slightly in order to analyze all impurities. The British Pharmacopoeia TLC method was not suitable because of its poor selectivity. Both OPLC and HPLC are suitable for purity testing. The OPLC method was preferred for process-validation because it shows a short analysis time and low eluent consumption (tenfold less than HPLC). The HPLC method was used for critical batches due to its better precision (3,5% RSD).

      Keywords:
      Classification: 4b, 13b
      120 061
      Planar yeast estrogen screen with resorufin-?-D-galactopyranoside as substrate
      D. SCHICK, W. SCHWACK* (*Inst. of Food Chem., Univ. of Hohenheim, Garbenstraße 28, 70599 Stuttgart, Germany, wolfgang.schwack@uni-hohenheim.de)

      J. Chromatogr. A 1497, 155-163 (2017). Development of a planar yeast estrogen screen for the determination of estrogen active compounds. Introduction of resorufin-β-D-galactopyranoside, providing the orange fluorescing resorufin after enzymatic cleavage, as the planar yeast estrogen screen (pYES) substrate to determine estrogen active compounds (EAC). For samples containing blue fluorescent components, this substance is better suited than the generally employed substrate 4-methylumbelliferyl-β-D-galactopyranoside, which delivered blue fluorescing 4-methylumbelliferone after enzymatic cleavage by the YES reporter β-D-galactosidase. The mean LOD and LOQ was 3.5 and 6.5 pg/zone for 17β-estradiol (1) and 17α-ethinylestradiol (2), respectively; recoveries were close to 100% for (1) and (2) from spiked water samples in a concentration range of 2–20 ng/L.

      Classification: 13b, 37c
      85 058
      Steroid structure and retention in normal- and reversed-phase thin-layer chromatography
      S.M. PETROVIC*, M. SAKAC, S. JOVANOVIC-SANTA, (*Dept. of Anal. Chem., Fac. of Techn., Univ. of Novi Sad, Cara Lanzara 1, P.O. Box 340, 21000 Novi Sad, Yugoslavia)

      J. Planar Chromatogr. 13, 106-113 (2000). Study of the chromatographic behavior of a series of (26) estradiol and estrone derivatives on silica gel with binary nonaqueous mobile phases and on C8- and C18-modified silica with binary aqueous mobile phases. HPTLC on silica gel or silica RP-8 resp. RP-18. Spots were observed under UV 254 nm.

      Keywords:
      Classification: 13b
      120 086
      Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections
      S. HAGE, Gertrud E. MORLOCK* (*Chair of Food Sci., Inst. for Nutr. Sci. & Interdisciplinary Res. Center (IFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. Chromatogr. A 1490, 201-211 (2017). HPTLC for comparison of the phenolic profiles of polar extracts from Populus nigra L. (1), Populus alba L. (2) and Salix alba L. (3) buds. Five chemotypical patterns were distinguished after derivatization with Natural Products reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, which directly linked to active molecules in the chromatograms. The results showed that polyvalent compounds were evident when all derivatization and activity assays were combined together. Detection of at least three antimicrobial compound zones using Aliivibrio fischeri and Bacillus subtilis bioassays and of one phyto-estrogen with the planar yeast estrogen screen in Populus buds. Detection of several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase in all samples. Bioactive compounds were assigned by HPTLC-MS, e.g. chrysin as selective cholinesterase inhibitor, and caffeic acid and galangin as antimicrobials in (1) and (2). The method is suitable to determine the botanical origin and quality of Populus bud extracts and propolis samples.

      Classification: 13b, 32e
      121 022
      Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology
      A. SCHOENBORN*, P. SCHMID, S. BRÄM, G. REIFFERSCHEID, M. OHLIG, S. BUCHINGER (ZHAW Life Sciences und Facility Management, Grüental, 8820 Wädenswil, Switzerland)

      J. Chromatogr. A 1530, 185-191 (2017). Development of a new spray-on method for applying yeast cells to HPTLC plates, leading to a much higher sensitivity of the planar yeast estrogen screen (p-YES), which can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. HPTLC of sample constituents and direct detection of estrogenic compounds by spraying with yeast cells. This resulted in much sharper signals compared to those in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU with reduced signal broadening, thus lower LOQ for estrogenic compounds, e.g. estrone 2 pg/zone, 17β-estradiol 0.5 pg/zone, 17α-ethinylestradiol 0.5 pg/zone and estriol 20 pg/zone. Demonstration of the possibility of the method to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility by using native samples from wastewater or even surface water directly applied on HPTLC plates without the need for prior sample treatment.

      Classification: 4e, 13b, 37c
      121 054
      Effect-based and chemical analytical methods to monitor estrogens under the European Water Framework Directive
      Sara KONEMANN*, R. KASE, E. SIMON, K. SWART, S. BUCHINGER, M. SCHLÜSENER, H. HOLLER, B. ESCHER, I. WERNER, S. AÏSSA, E. VERMEIRSSEN, V. DULIO, S. VALSECCHI, S. POLESELLO, P. BEHNISCH, B. JAVURKOVA, O. PERCEVAL, C. DI PAOLO, D. OLBRICH, E. SYCHROVA, R. SCHLICHTING, L. LEBORGNE, M. CLARA, C. SCHEFFKNECHT, Y. MARNEFFE, C. CHALON (*Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany, sarah.koenemann@rwth-aachen.de)

      Trends Anal. Chem. 102, 225-235 (2018). The study described reliable effect-based methods for screening of endocrine disrupting compounds to fulfill the requirements of the European Decision EU 2015/495 regarding steroidal estrogens of the Water Framework Directive. The study identified TLC-based methods such as the planar Yeast Estrogen Screen (pYES) for the quantification of the overall estrogenic activity present in the sample by means of 17ß-estradiol-equivalence concentrations.

      Classification: 13b
      102 037
      Evaluation of visualizing reagents for estradiol on thin layer by densitometric method
      Alina PYKA*, W. KLIMCZOK, D. GURAK (*Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Silesia, 4 Jagiellonska Street, 41-200, Sosnowiec, Poland; apyka@slam.katowice.pl)

      J. Liq. Chromatogr. Relat. Technol. 31, 555-566 (2008). Five new derivatization reagents (gentian violet, methylene violet, methylene blue, malachite green, and Janus blue) were used to detect estradiol on aluminium oxide. Barton’s reagent, rhodamine B, and sulfuric acid were used as the comparative derivatization reagents. Limit of detection, detection index, modified broadening index, modified contrast index, and linearity range were determined for estradiol after derivatization with these reagents. Quantitative determination by absorbance measurement between 200 and 600 nm.

      Classification: 13b