Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      129 015
      Comparison of yeast estrogen screening on HPTLC and in microtiter plates
      A. BERGMANN*, E. SIMON, A. SCHIFFERLI, A. SCHÖNBORN, E. VERMEIRSSEN (*Swiss Centre for Applied Ecotoxicology, Eawag, Überlandstrasse 133, 8600 Dübendorf, Switzerland, alanjames.bergmann@oekotoxzentrum.ch)

      CBS 125. HPTLC of estrogen-active compounds in a migrate of a food contact material on silica gel with chloroform - acetone - petroleum ether 11:4:5. Planar Yeast Estrogen Test (P-YES) was performed by spraying 2 mL yeast cells onto HPTLC plates, followed by incubation at 30 °C for 3 h and spraying with 2 mL 0.5 mg/mL 4-methylumbelliferyl-beta-D-galactopyranoside and incubation for 20 min at 37 °C. The method was compared with microtiter bioassay (L-YES). P-YES was more sensitive than L-YES and results can be repeated upto one year later.

      Classification: 4e, 13b
      129 029
      New solvent systems to separate some estrogenically active compounds by high‑performance thin‑layer chromatography (HPTLC)
      B. SPANGENBERG (Institute of Process Engineering, Offenburg University of Applied Sciences: Hochschule Offenburg, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 35, 189-195 (2022). Planar yeast estrogen screen (pYES) of estriol, daidzein, genistein, 17β-estradiol, 17α-ethinyl estradiol, estrone, 4-nonylphenol and bis(2-ethylhexyl) phthalate on silica gel with cyclohexane - methyl ethyl ketone 2:1 or cyclohexane - cyclopentyl-methyl ether 3:2. Detection under UV light at 272 nm.

      Classification: 13b
      127 030
      High-performance thin-layer chromatography in combination with a yeast-based multi-effect bioassay to determine endocrine effects in environmental samples
      N. BAETZ, L. ROTCHE, V. WIRZBERGER, B. SURES, T. SCHMIDT, J. TUERK* (*Institute of Energy and Environmental Technology, Bliersheimer Str. 58 – 60, 47229 Duisburg, Germany, tuerk@iuta.de)

      Anal. Bioanal. Chem. 413, 1321-1335 (2021). HPTLC of estrone (1), 17β-estradiol
      (2), 17α-ethinylestradiol (3), 5α-dihydrotestosterone (4), and progesterone (5) in wastewater and surface water samples on silica gel with a 
      mixture of dichloromethane, cyclohexane, and acetone in different proportions. Detection by spraying with 8 % sulfuric acid in ethanol, followed by heating at 105 ºC for 10 min. Qualitative identification under UV light at 310 nm. Yeast multi endocrine-effect screen was performed by spraying the HPTLC plates with a mixed suspension of genetically modified Arxula adeninivorans yeast strains, which contain either the human estrogen, androgen, or progesterone receptor. The HPTLC plates were incubated at 30 ºC for 18 h and at 100 % humidity. After incubation, densitometric evaluation at: 445/K460 nm, 475/K500 nm and 542/K560 nm to determine the fluorescence of the cyan fluorescent protein (CFP, gestagen), green fluorescent protein (GFP, androgen), and DsRed2 protein (estrogen), respectively. The hRF values for (1) to (5) were 21, 22, 29, 34 and 39, respectively.

       

       

      Classification: 13b
      123 009
      Equol determination in cattle manure by HPTLC-DART-TOF-MS
      V. PETERS, B. SPANGENBERG* (*Department of Process Engineering, University of Offenburg, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Liq. Chromatogr. Relat. Technol. 42, 311-316 (2019). HPTLC of equol in cattle manure with methyl t-butyl ether - cyclohexane 1:1. The plate was scanned with a Time of Flight – Direct Analysis in Real Time – Mass Spectrometry (TOF-DART-MS) system. The hRF value of equol was 71. The LOD and LOQ for equol were 2.4 µg/zone and 4.5 µg/zone, respectively. 

      Classification: 13b
      112 014
      Coupling sample preparation with effect-directed analysis of estrogenic activity – proposal for a new rapid screening concept for water samples
      A. SCHOENBORN*, A. GRIMMER (*Zurich University of Applied Sciences, P.O. Box, 8820 WAedenswil, Switzerland, andreas.schoenborn@zhaw.ch)

      J. Planar Chromatogr. 26, 402-408 (2013). HPTLC of 17alpha-ethinylestradiol (1) and 17beta-estradiol (2) and estrone (3) on silica gel with chloroform - acetone - petroleum 11:4:5. The method was combined with the Yeast Estrogen Screen for the detection of estrogenic activity in aqueous samples. Quantitative determination by absorbance measurement at 366 nm. The hRf values of compounds (1) to (3) were 60, 50 and 67, respectively. LOD for (2) was 5 pg/zone.

      Classification: 4e, 13b
      62 094
      A multi-residue TLC screening procedure for anabolic estrogens and detection of estradiol, DES or zeranol in chicken muscle tissue extracts
      M.B. MEDINA, D.P. SCHWARTE, (Eastern Reg. Cent., Agric. Res. Serv., Philadelphia, PA 19118 USA)

      Food Addit. Contam. 4, 415-427 (1987). HPTLC of 17ß-estradiol, DES, zearalanol (zeranol), zearalenone and their metabolites estrone etc. on silica with methyl chloride - methanol - isopropanol 97:1:2. Detection by exposure to iodine vapor and spraying with 1% starch solution. Detection limit, 4 ng.

      Classification: 13b
      112 015
      Combination of high-performance thin-layer chromatography with a specific bioassay – a tool for effect-directed analysis
      D. SPIRA*, G. REIFFERSCHEID, S. BUCHINGER (*Federal Institute of Hydrology, Am Mainzer Tor 1, 56068 Koblenz, Germany, spira@bafg.de)

      J. Planar Chromatogr. 26, 395-401 (2013). HPTLC of 17alpha-ethinylestradiol (1), 17beta-estradiol (2) and estrone (3) on silica gel with chloroform - acetone - petroleum 11:4:5. The method was combined with the Yeast Estrogen Screen for the detection of estrogenic effects in sediment extracts of a river. Quantitative determination by absorbance measurement at 320 nm. The hRf values of compounds (1) to (3) were 74, 61 and 84, respectively.LOD and LOQ were calculated for the estrogenic model compound (1): LOD was 0.46 pg without prior development of the TLC plate and 0.48 pg after development, LOQ was 0.8 pg without development and 1.6 pg after development.

      Classification: 4e, 13b
      64 095
      Weibliche Sexualhormone
      B. UNTERHALT*, A. SANATGAR, (Inst. für Pharm. Chem., Hittorfstr. 58-62, D-4400 Münster, FRG)

      Zur DAB-Reinheitsprüfung „Verwandte Substanzen“ und zur DAB- Identitätsprüfung mit DC. (Female sexual hormones. For DAB-purity test of „similar substances“ and for DAP-identity test with TLC.) (German). Deutsche Apotheker Zeitung 129, 1998 (1989). TLC of ethisterone, norethisterone, mestranole, estradiolbenzoate, ethi- nylestradiole on silica with acetone - chloroform 1:9. Visualization under UV 254 and 366 nm.

      Classification: 13b