J. Planar Chromatogr. 11, 305-308 (1998). Investigation of the chromatographic behavior of dexamethasone, prednisolone, and other representative corticosteroids. - 12 Different mixtures of organic solvents were compared to assess their efficiency as mobile phases for the separation of 18 glucocorticosteroids by TLC. Optical evaluation of the plates after treatment with 4 different spray reagents then revealed that the combination of choice for optimum separation and detection was chloroform- methanol 23:2, or chloroform - acetone 9:1 and a mixture of 2,4-dihydroxybenzaldehyde, sulfuric acid, and acetic acid as spray reagent. The specificity of these chromatographic conditions was assessed by analysis of 35 anabolic steroids and 10 other veterinary drugs. No significant interferences were found.

J. Chromatogr. 295, 308-311 (1984). TLC separation of steroids which differ by only one double bond on silver nitrate impregnated silica with benzene - ethanol 9:1, benzene - ethyl acetate 2:1, ether - benzene 2:1, hexane - ethyl acetate 1:1 and other solvent systems. Detection with ethanol - sulfuric acid 1:1. Also TLC of steroids after bromination on the plate.

Acta Pharmaceutica Hungarica 59, 167-172 (1989). TLC of 2ß,16ß-bis-(4'-dimethyl-1‘piperazino)-3a,17ß-diacetoxy-5a-andro stane (=Arduan) on silica with chloroform - dichloromethane - methanol 6:2:2. NaI in an unsaturated development tank sealed airtight. Determination of radioactivity of the parent drug and metabolites separated by ion-pair TLC.

Dtsch. Apoth. Ztg. 139, 517-518 (1999). Approved procedure for identity and quality test TLC and HPTLC of betamethasone 17a-valerate (using prednisolone acetate, hydroxycortisone acetate or dexamethasone acetate as reference substances) on silica gel with ethyl methyl ketone - toluene 2:3; without chamber saturation, bandwise application. The plate is left in the air for 15 min after application. Detection under UV 254 nm, after spraying with ethanolic sulfuric acid solution and heating at 110°C, and under UV 360 nm.

behavior of some steroids by OPLC. Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 90 (1984). OPLC (OPTLC) of progesterone, cholesterol, corticosterone, aldosterone, cortisol, cortisone on silica with a) petrol ether - ethyl-acetate 6:4, b) chloroform - ethyl acetate - ethanol 90:10:1, c) chloroform - phenol 9:1. Detection with 50 % phosphoric acid and heating at 105 °C for 15 minutes.

J. of Medicinal Chemistry 32, 1977-1981 (1989). TLC of pregnane derivatives on silica with 25-75% ethyl acetate in hexane and methanol - dichloromethane 1:9.

Apoth. Ztg. 139, 628-629 (1999). HPTLC of budesonide with prednisolone acetate, dexamethasone acetate or betamethasone 17a-valerate as reference substance on silica gel with ethyl methyl ketone - toluene 2:3 without chamber saturation, bandwise application. The plate is left for 15 min in the air after application. Detection under UV 254 nm, after spraying with 20% ethanolic sulfuric acid and heating at 110°C for 5 min. Visualization under day light and under UV 360 nm.

Acta Chimica 117, 287-292 (1984). TLC of steroidal derivatives on silica with aq. perchloric acid solution. Identified substances: 5,6- beta dihydroxy-5-alpha -cholestane, its 3 beta-acetoxy, 3 beta-chloro-analogue; 5-hydroxy-6 beta-acrylamide-5 alpha-cholestane, its 3 beta-acetoxy, 3 beta-chloro analogue.