60th Indian Pharmaceutical Congress PA-197, (2008) HPTLC of medroxyprogestrone acetate in bulk and injectable dosage form on silica gel with toluene - ethyl acetate - ammonia 800:200:1. Quantitative determination by absorbance measurement at 240 nm. The linearity was in the range of 50-1800 ng/spot. Recovery was 100.1 %. In the stability test (acid, base, peroxide, thermal, photodegradation) the compound was well separated from degradation products. The method was suitable for routine quality control and for monitoring stability.

Advances in Liquid Chromatography, Sept., 3-6 (1988), Szeged, Hungary. TLC of testosterone, 5-dehydroandrosterone and androsterone on cyano- and amino- modified precoated silica with hexane - acetone 80:20. Detection by fluorescence at 360 nm after spraying with 5 % perchloric acid in methanol and heating at 110°C for 5 min.

Phytochemical Analysis 4, 76-81 (1993). TLC of fractions issued from an ethyl acetate extract of Mandevilla velutina (Apocynaceae) on silica with hexane - chloroform 1:4. Isolation of a new pregnane type steroid by PTLC on silica with hexane - diisopropyl ether - acetone 4:4:3. Detection with 10% sulfuric acid in methanol, followed by 3% phosphomolybdic acid in methanol.

CBS 101, 5-7 (2008). HPTLC of steroids on RP-18W and RP-18 with methanol - water 3:2 in a flat bottom chamber. Detection by spraying with perchloric acid (20 % in ethanol) followed by heating at 100 °C for 5 min. Migration time on the hydrophobic RP-18 layer was 130 min whereas on the water-wettable RP-18W layer it was 39 min. The maximal water content of the mobile phase is 40 % for RP-18 layers and up to 100 % for RP-18W. Separation of steroids was better on RP-18W. The hRf value of stanozolol was 4, of methyl testosterone 12, of Reichstein’s S 26, of hydrocortisone 37 and cholesterol remained at the application position.

J. of Medicinal Chemistry 32, 203-213 (1989). TLC on silica with ether - petrol ether 1:1, chloroform - ether - methanol 90:8:2 and petrol ether - ethyl acetate - methanol 50:45:5. Visualization by UV; iodine vapor or by immersion in 50% phosphoric acid and heating for 30 min. at 120°C.

J. Planar Chromatogr. 6, 228-231 (1993). OPLC of potassium canrenoate, canrenoic acid and canrenone on silica with different mixtures of methanol and 0.01 M phosphate buffer (pH 5.0). Quantification by densitometry at 285 nm.

J. Liq. Chromatogr. Relat. Technol. 31, 2673-2685 (2008). TLC of dehydroepiandrosterone on silica gel with chloroform - acetone 17:3 with chamber saturation for 30 min. Detection by dipping into 0.05 % aqueous solutions of visualizing agents (methylene violet, gentian violet, janus blue, methylene blue, malachite green, rhodamine B) and methanolic chloramine T/methanolic sulfuric acid solution. Quantitative determination by densitometry at 200 nm (before derivatization and for methylene violet), 657 nm (gentian violet), 487 nm (janus blue), 488 nm (malachite green), 580 nm (rhodamine B), and 361 nm (chloramine T). LOD was 0.29 µg/zone (without visualizing agent, with chloramine T and with rhodamine B), 4.19 µg/ zone with methylene violet, 1.10 µg/zone with gentian violet, 2.14 µg/zone with janus blue, and 4.19 µg/zone with malachite green, all with an application volume of 5 µL.

Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 8, 337-340 (1988). TLC on silica with dichloromethane - acetone 8:1 and 45:1. Detection by spraying with sulfuric acid - ethanol - water and heating at 120°C for 10 min. Quantification by densitometry at 550 nm.