Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      82 058
      Comparative study of the thin-layer chromatographic detection of different corticosteroids
      O. HUETOS, T. REUVERS*, J.J. SANCHEZ, (Unidad de Residuos Zoosanitarios, Centro Nacional de Alimentación (ISCIII), Ctra. Pozuelo-Majadahonda Km. 2, 28220-Majadahonda, Spain)

      J. Planar Chromatogr. 11, 305-308 (1998). Investigation of the chromatographic behavior of dexamethasone, prednisolone, and other representative corticosteroids. - 12 Different mixtures of organic solvents were compared to assess their efficiency as mobile phases for the separation of 18 glucocorticosteroids by TLC. Optical evaluation of the plates after treatment with 4 different spray reagents then revealed that the combination of choice for optimum separation and detection was chloroform- methanol 23:2, or chloroform - acetone 9:1 and a mixture of 2,4-dihydroxybenzaldehyde, sulfuric acid, and acetic acid as spray reagent. The specificity of these chromatographic conditions was assessed by analysis of 35 anabolic steroids and 10 other veterinary drugs. No significant interferences were found.

      Keywords:
      Classification: 13a
      117 080
      Modern direct bioautography
      of endocrine active compounds
      Ines KLINGELHOEFER, Gertrud MORLOCK* (*Justus Liebig University Giessen, Institute of Nutritional Science, IFZ, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, gertrud.morlock@uni-giessen.de)

      CBS 115, 2-4 (2015). HPTLC of propolis tinctures and standards estrone, 17-estradiol, 17-ethinylestradiol, estriol, bisphenol A, 4-n-nonylphenol, and caffeic acid methyl ester on RP-18W with n-hexane – toluene – ethyl acetate 8:3:2 to a migration distance of 7 cm. For the HPTLC-pYES bioassay the plate was dipped into a yeast cell suspension and incubated for 3 h. Biodensitometric evaluation by fluorescence measurement at 365 nm with a cut-off filter of 400 nm. Elution of the bioactive zones with methanol – ammonium formate buffer (10 mM, pH 4, 49:1) into a ESI-MS. The LOD for 17-estradiol was 0.2 pg/zone and the LOQ 0.5 pg/zone. With this method estrogen effective substances can be detected with no or minimal sample preparation.

      Classification: 13b
      70 090
      Some technical recommendations for improving the performance of quantitative thin-layer chromatography
      G. WINDHORST, J.P. KLEIJN*, (*Quality Assurance, Anal. Dev. Act., Organon Intern. BV, Oss, The Netherlands)

      J. Planar Chromatogr. 5, 229-233 (1992). TLC of a bis quaternary ammonium steroid on silica with lithium perchlorate (20%) in water - 2-propanol - glycerol 5:90:5. Separation of the decomposition products with sodium iodide (40%) in water - 2-propanol - acetonitrile 5:90:5. Detection with iodine vapor resp. by immersion into a solution of iodine (0.25%) in chloroform - methanol 1:1 (decomposition products). TLC of an estrogen and a progestogen on silica with toluene - ethyl acetate - acetic acid 80:20:3. Quantification by scanning in fluorescence/reflection mode. Practical hints on application, development, detection, and densitometric evaluation; if performed carefully, TLC results are of the same quality as those from HPLC or GC.

      Classification: 13b
      113 030
      Estimation of antioxidant potential of individual components present in complex mixture of Withania somnifera (Ashwagandha) root fraction by thin-layer chromatography–2,2-diphenyl-1-picrylhdrazyl method
      S. DEVKAR, S. JAGTAP, S. KATYARE, M. HEDGE* (*Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Medical College Campus, Pune 411043, India, mahabaleshwarh@yahoo.com)

      J. Planar Chromatogr. 27, 157-161 (2014). HPTLC of withanoside V (1), withaferine A (2), 1,2-deoxywithastramonolide (3), withanone (4), withanolide A (5), and withanolide B (6) on silica gel with dichloromethane - toluene - methanol - acetone - diethyl ether 13:14:8:3:1. Quantitative determination by absorbance measurement at 235 nm. The hRF values for (1) to (6) were 7, 55, 61, 63, 66 and 77, respectively. Precision was below 2 % (n=3). The LOD and LOQ were 60 and 250 ng/zone for (1), 120 and 350 ng/zone for (2), 80 and 300 ng/zone for (3), (4) and (6), and 60 and 250 ng/zone for (5), respectively. Average recoveries for (1) to (6) were 97, 98, 99, 98, 98 and 99 %, respectively.

      Classification: 13c
      121 053
      High-performance thin-layer chromatography of four sterols in a medicinally important grass species – Heteropogon contortus (L
      N. KAUR*, R. GUPTA (*Department of Botany, Punjabi University, 147002
      Patiala, India, navjot21188@gmail.com)

      J. Planar Chromatogr. 31, 143-149 (2018). HPTLC of stigmasterol (1), β-sitosterol (2), campesterol (3) and ergosterol (4) in Heteropogon contortus on silica gel with toluene – methanol – formic acid 90:10:3. Detection by spraying with anisaldehyde sulfuric acid followed by heating at 110-120 °C for 4-5 min. Quantitative determination by absorbance measurement at 530 nm. The hRf values for (1) to (4) were 23, 13, 39 and 28, respectively. Linearity was in the range of 2-12 ng/zone for (1) to (4). The LOD and LOQ were 50 and 200 pg/zone for (1), 13 and 40 pg/zone for (2), 86 and 260 pg/zone for (3) and 20 and 60 pg/zone for (4), respectively. Recovery was in the range of 98.4 and 99.6 % for (1) to (4).

      Classification: 13c
      56 071
      A complete separation of lipids by three-directional thin-layer chromatography
      J. KRAMER, R. FOUCHARD, E. FARNWORTH

      Lipids 18, 896-899 (1983). Two-dimensional separation of phospholipids on silica with 1) chloroform - methanol - NH3 65:25:5., 2) chloroform - acetone - methanol - acetic acid - water 10:4:2:3:1; visualizing under UV after spraying with 2',7'-dlchlorofluoresceine; removing the fractions by scraping and identification by co-chromatography with authentic standards (one-dimensionally); plasmalogenes can be identified by exposing the plate to HCl (after the first development).

      Classification: 11a, 13c
      63 063
      Chemical constituents of some species of Agaricaceae
      F. SENATORE, (Dipartimento di Chimica delle Sostanze Naturali, Univ. di Napoli, via Domenico Montesano 49, I-80131 Napoli, Italy)

      Biochemical systematics and ecology 16, 601-604 (1988). TLC of sterols on silica and AgNO3-silica with petrol ether - ethyl acetate 35:5 and benzene - hexane 1:1.

      Classification: 13c
      68 099
      Chemosystematic differentiation of Quercus ilex and Q
      Z.A. RAFITI*, E. ZAVARIN, Y. PELLEAU, (*4a 1152, Institut Méditerranéen d’Ecologie et de Paleoécologie, Univ. d’Aix-Marseille III., France)

      Biochemical Systematics and Ecology 19, 249-252 (1991). TLC of cholesterol, stigmasterol, ß-sitosterol, D5-avenasterol, D7-stigmasterol and brassicasterol on silica with chloroform - ethanol 50:1.

      Classification: 13c