Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      72 091
      True color photodocumentation of UV-irradiated thin layer chromatograms
      Z. VEGH, (Chemical Works of Gedeon Richter Ltd., H-1475 Budapest 10, P.O. Box 27, Hungary)

      J. Planar Chromatogr. 6, 341-345 (1993). Use of color slide films for the selection of appropriate filters and exposure data. Color prints are then made with color negative film using the previously optimized condition. With this method the number of photographs can be limited to one of each chromatogram. TLC of steroids (hydrocortisone, prednisolone, mesylate, hydrocortisone acetate, and spironolactone, trenbolone acetate, hydroxyprogesterone caproate, pregnadienolone acetate oxime ) with benzene - ethyl acetate 1:1. Visualization by spraying with a 10% ethanolic solution of sulfuric acid, followed by heating at 100 °C for 2 to 4 min.

      Keywords:
      Classification: 3, 13
      85 056
      Unsaponifiable lipid constituents of some underutilized tropical seed oil
      K.O. ESUOSO*, H. LUTZ, E. BAYER, M. KUTUBUDDIN, (*Inst. of Org. Chem., Res. Centre for Nucleic Acid and Peptide Chem., Univ. of Tuebingen, 72076 Tübingen, Germany)

      J. Agric. Food Chem. 48, 231-234 (2000). TLC of the unsaponifiables (hydrocarbons, triterpene alcohols, sterols) on silica gel, bandwise applied, with hexane - ethyl acetate 6:1. Detection after spraying with 0.5% Rhodamine 6G solution and observation under UV. After elution and extraction silanation of sterols and triterpene alcohols with BSTFA for GC investigation.

      Keywords:
      Classification: 13, 15
      56 080
      Characterization of C19 steroids by two-dimensional thin-layer chromatography
      W. NIENSTEDT

      J. Chromatogr. 329, 171-177 (1985). Two-dimensional TLC of unconjugated metabolites of neutral steroids on silica with 1) 1,2-dichloroethane - methyl acetate 8:2 and 2) hexane -1-hexanol 65:35. Detection with Liebermann-Burchard reagent.

      Keywords:
      Classification: 13a
      65 082
      Improved Chromatographic clean-up of anabolics in bovine urine
      H.F. DE BRABANDER*, P. VANHEE, S. VAN HOYE, R. VERBEKE, (*Laboratory of Chemical Analysis of Food from Animal Origin, Vet. Fac.of the Univ. of Ghent, Casinoplein 24, B-9000 Ghent, Belgium)

      J. Planar Chromatogr. 2, 33-38 (1989). Two-dimensional TLC of anabolics on silica with 5 different solvent systems. Reference mixtures contained 2 - 20 ng of the steroids (zeranol, zearalenone, a-nortestosterone, medroxyprogesterone acetate, trenbolone, trebolone acetate). Alternatively a „4x4“ developing mode could be used. In this mode 4 samples are developed in two dimensions on one HPTLC plate. For inducing fluorescence reaction, the plates were dipped into a 5% sulfuric acid - ethanol solution for 30 s and then incubated at 95°C for 10 min. The fluorescence before and after heating was observed under UV 366 nm. The described method permit the routine detection of various anabolic residues in bovine urine at levels of 0.5 - 10 ppb. The recoveries are high (70 - 90%). Standard deviation were between 2 and 8%.

      Classification: 13a, 13b
      94 030
      Determination of progesterone in drug release media
      A. JAMSHIDI (Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, P.O. Box 14965/115, Tehran, Iran, a.jamshidi@ippi.ac.ir)

      CBS 93, 10-12 (2004). HPTLC of progesterone on silica gel prewashed by development in AMD2 first with chloroform - methanol 1:1 and then with the mobile phase, followed by drying at 80 °C for 15 min. Development in AMD2 with toluene - 2-propanol 10:1 without preconditioning over 60 mm. Quantitative determination by absorbance measurement at 252 nm followed by spectra recording from 200 to 360 nm. The linear working range is 25.7-154.5 ng/zone. Repeatability (standard deviation calculated from the amounts of seven simulated progesterone samples determined on the same plate at three concentration levels in the lower, middle and upper range) is 0.26-1.29 %. Recovery is 99.88-100.97 %. Reproducibility was performed with recycled HPTLC plates.

      Classification: 13a, 32a
      120 060
      Two-dimensional thin-layer chromatography of phytoestrogens on RP-18 W plate, detected by effect-directed analysis using the yeast estrogen screen test
      Y. MINAR, B. SPANGENBERG* (*University of Offenburg, Department of Process Engineering, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 30, 423-426 (2017). 2D-HPTLC of phytoestrogenic active compounds in the root of Glycyrrhiza glabra on RP-18 with hexane – ethyl acetate – acetone 9:3:2 in the first direction and acetone – water 3:2 in the second direction. Effect-direct analysis by dipping into a yeast suspension followed by incubation at 30 °C for 4 h, drying at 37 °C for 15 min and spraying with the combined reaction buffer C (20 mL reaction buffer C is mixed with 0.2 mL of a freshly prepared solution of 0.05 g/mL 4-methylumbelliferyl-ß-D-galactopyranoside in DMSO or 0.2 mL of a freshly prepared solution of 0.05 g/mL 5-bromo-4-chloro-3-indoxyl-ß-D-galactopyranoside (X-Gal) in DMSO). Fluorescence detection at UV 366 nm._x000D_

      Classification: 13b
      85 057
      Chromatographic methods in pharmaceutical purity testing
      K. FERENCZI-FODOR, B. BAGOCSI, D. FABIAN, E. GÜRTLER, Z. VEGH, (Gedeon Richter Ltd., P.O.Box 27, 1475 Budapest 10, Hungary)

      Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 19-25. OPLC of ethinyl estradiole on silica gel with cyclohexane - ethyl acetate - chloroform 3:1:1. Detection by spraying with sulfuric acid and visual evaluation at 366 nm. The method is compared with the Official British and European Pharmacopoeia monographs. Only with the OPLC method it was possible to separate all impurities, i.e. 6-hydroxy derivatives (both isomers), the oxo-derivatives (6-oxo- and 16-oxo-), the 9(11)-dehydro and the 17-epi-derivatives from the estrone and estradiol. The HPLC method of the 3rd European Pharmacopoeia has to be changed slightly in order to analyze all impurities. The British Pharmacopoeia TLC method was not suitable because of its poor selectivity. Both OPLC and HPLC are suitable for purity testing. The OPLC method was preferred for process-validation because it shows a short analysis time and low eluent consumption (tenfold less than HPLC). The HPLC method was used for critical batches due to its better precision (3,5% RSD).

      Keywords:
      Classification: 4b, 13b
      116 028
      Isolation, cytotoxic evaluation, and
      simultaneous quantification of eight bioactive secondary metabolites from Cicer
      microphyllum by high-performance thin-layer chromatography
      A. DAR, S. RATH, A. QAUDRI, B. SINGH, S. TASDUQ, A. KUMAR, P. SANGWAN* (*Bio-organic Chemistry Division, CSIR-Indian Institute of Integrative Medicine (CSIR-IIIM),
      Jammu Tawi, India, plsangwan@iiim.ac.in)

      J. Sep. Sci. 38, 4021-4028 (2015). HPTLC of eight natural products viz. stigmasterol (1), oleanolic acid-3-acetate (2), oleanolic acid (3), biochanin A (4), genistein (5), pratensein (6), chrysoeriol (7), and luteolin (8) in Cicer microphyllum on silica gel with n-hexane - ethyl acetate - formic acid 90:65:8. Detection by dipping into a solution of cerric ammonium sulfate reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 366 nm. The hRF values for compounds (1) to (8) were 98, 89, 78, 70, 63, 42, 33 and 4, respectively. Linearity was in the range of 100-800 ng/zone for (1) to (8). LOD and LOQ were 60 and 90 ng/zone for (1), (5) and (8), 50 and 90 ng/zone for (2), 90 and 110 ng/zone for (3), 70 and 100 ng/zone for (4) and (6) and 80 and 110 ng/zone for (7). The intermediate precision was below 1.5 % (n=3). Recovery was in the range of 98-100 %.

      Classification: 8b, 13c