J. Chromatogr. 350, 468-470 (1986). TLC of various steroids on silica with chloroform - methanol 10:1. Detection with 1 % solution of rubeanic acid in conc. sulfuric acid. Detection limit up to 100 ng for various types of steroids. Densitometric determination at 254 nm.

J. Planar Chromatogr. 3, 34-37 (1990). Investigation of the retention behavior of 12 steroids (cholesterol, allylestrenol, pregnanediol, progesterone, estradiol, estrone, estriol etc.) using PR-HPTLC systems with acetonitrile – methanol, acetonitrile – water, and methanol – water binary mixtures. The separation abilities of the mobile phases considered were studied using the principal component analysis method. Visualization by spraying with a mixture of 10 g copper sulfate and 5 mL o-phosphoric acid (86%) dissolved in 95 mL methanol.

J. Liq. Chromatogr. Relat. Technol. 29, 2035-2044 (2006). TLC of androsterone, epi-androsterone, dehydro-epi-androsterone, testosterone, stigmasterol, beta-sitosterol, estradiol, hydrocortisone, and cholesterol on silica gel with chloroform - acetone 17:3 and activation at 100 °C, 120 °C, 150 °C, and 200 °C during 15, 30, 60, and 120 min. Activation time temperature influenced Rf values and order of separated compunds.

J. Chromatogr. A 1490, 201-211 (2017). HPTLC for comparison of the phenolic profiles of polar extracts from Populus nigra L. (1), Populus alba L. (2) and Salix alba L. (3) buds. Five chemotypical patterns were distinguished after derivatization with Natural Products reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, which directly linked to active molecules in the chromatograms. The results showed that polyvalent compounds were evident when all derivatization and activity assays were combined together. Detection of at least three antimicrobial compound zones using Aliivibrio fischeri and Bacillus subtilis bioassays and of one phyto-estrogen with the planar yeast estrogen screen in Populus buds. Detection of several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase in all samples. Bioactive compounds were assigned by HPTLC-MS, e.g. chrysin as selective cholinesterase inhibitor, and caffeic acid and galangin as antimicrobials in (1) and (2). The method is suitable to determine the botanical origin and quality of Populus bud extracts and propolis samples.

PLoS ONE 8(11), e80841 (2013). On silicic acid columns, total ceramides, from methanol – chloroform extracts of mouse tissue (brain, heart, liver) and of cell cultures (astrocytes, oligodendroglia) were purified from other lipids (phospholipids, sphingoid bases, glycosphingolipids, retained on the column) and fractioned using chloroform – acetone – acetic acid 24:1:0.01 followed by chloroform – acetone – acetic acid 18:2:0.01. For the monitoring, TLC of fractions (layer not specified) with chloroform – methanol – acetic acid 190:10:1. After derivatization by iodine vaporization or by spraying with copper sulfate reagent (copper(II) sulfate 20 mM - sulfuric acid 5 % - ammonium molybdate 1 %), lipids are detected as orange or brown-black zones, respectively. The aforesaid fractionation allowed the isolation of hydroxyl-fatty-acyl ceramides, phytoceramides, non-hydroxy-fatty-acyl ceramides and cholesterol (identification by comparison to standards and by GC-MS); their hRf values were between 40 and 80. Quantitative determination by densitometric measurement. This is the first report of phytoceramide identified in vertebrate brain, heart and liver.

Pharmacogn. Mag. 14, 45-51 (2018). HPTLC of stigmasterol in Monochoria vaginalis and Monochoria hastata on silica gel with chloroform – methanol 4:1. Detection by spraying with anisaldehyde-sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for stigmasterol was 30. Linearity was between 1000 and 5000 ng/zone. LOD and LOQ were 80 and 200 ng/zone. The intermediate precision was <3 % (n=3). Average recovery was 99.8 %.

J. Biochem. (Tokyo) 99, 771-775 (1986). Reversed-phase TLC of cholestanol after conversion to alpha and beta -epoxides with m-chloroperbenzoic acid. Detection by spraying with phosphomolybdic acid. Quantification by densitometry. The calibration curves were linear 100-1000 ng. Correlation among TLC, GC/MS, and GC methods was 1:1:1. TLC method is useful for primary diagnostic screening of CTX.

Phytochemistry 28, 143-154 (1989). TLC of desmethyl sterols on silica with butanol - hexane 2:8. Detection by spraying with 1% 2',7'-dichlorofluorescein in ethanol and visualization under long wave UV.