J. Planar Chromatogr. 7, 164-165 (1994). HPTLC of clobetasol propionate on silica with chloroform and, after intermediate drying at 60 °C, with chloroform - diethyl ether 3:1. Quantification by densitometry (absorbance at 250 nm.)

Chromatographia 52 (11/12), 815-817 (2000). Use of 2D-TLC to screen plant samples for ecdysteroids, giving satisfactory separation even in the presence of substantial impurities and for mixtures containing many ecdysteroids, on silica gel for apolar ones and on RP-18 for polar ones, using a small amount of water in the mobile phase in the first direction, then a water-free mobile phase in the second. Discussion of the advantages of the method.

Chem. (Bunseki Kagaku) 34, 612-618 (1985). (Japanese). (High-purity, high-recovery purification of prednisolone by the automatic recrystallization method and its purity evaluation by thin-layer chromatography and by differential scanning colorimetry.) TLC of prednisolone on silica with chloroform - methanol 9:1. Detection by UV 254 nm.

J. Chromatogr. 465, 448-450 (1989). Two-dimensional TLC of title compounds on silica with ethyl acetate - chloroform 2:1 for the 1st direction and benzene - chloroform - methanol - 5 M acetic acid 70:20:20:1 for the 2nd. Detection of the fluorescent spots under UV light.

J. Liq. Chromatogr. Relat. Technol. 29, 1891-1903 (2006). HPTLC of androsterone, epi-androsterone, dehydro-epi-androsterone, testosterone, stigmasterol, beta-sitosterol, estradiol, hydrocortisone, and cholesterol on RP-18 W with methanol - water, and acetonitrile - water in different composition, with chamber saturation. Detection by spraying with sulfuric acid - methanol 1:9 and heating at 120 °C for 15 min. Densitometric determination of RF values. The aim of the work was to compare the lipophilicity of selected steroids determined by RP-HPTLC on RP-18 W plates using different mobile phases with lipophilicity values estimated by computational methods.

J. Chromatogr. A 1497, 155-163 (2017). Development of a planar yeast estrogen screen for the determination of estrogen active compounds. Introduction of resorufin-β-D-galactopyranoside, providing the orange fluorescing resorufin after enzymatic cleavage, as the planar yeast estrogen screen (pYES) substrate to determine estrogen active compounds (EAC). For samples containing blue fluorescent components, this substance is better suited than the generally employed substrate 4-methylumbelliferyl-β-D-galactopyranoside, which delivered blue fluorescing 4-methylumbelliferone after enzymatic cleavage by the YES reporter β-D-galactosidase. The mean LOD and LOQ was 3.5 and 6.5 pg/zone for 17β-estradiol (1) and 17α-ethinylestradiol (2), respectively; recoveries were close to 100% for (1) and (2) from spiked water samples in a concentration range of 2–20 ng/L.

J. Planar Chromatogr. 13, 106-113 (2000). Study of the chromatographic behavior of a series of (26) estradiol and estrone derivatives on silica gel with binary nonaqueous mobile phases and on C8- and C18-modified silica with binary aqueous mobile phases. HPTLC on silica gel or silica RP-8 resp. RP-18. Spots were observed under UV 254 nm.

PLoS ONE 9(1), e1003109 (2013). In order to understand how Borrelia burgdorferi acquires cholesterol from its host, five experiments were performed. HPTLC on silica gel with chloroform – methanol 17:3, the lipids were extracted through the Bligh and Dyer method: (1) from the spirochete incubated 4 h in a medium containing 4 mg/L BODIPY-cholesterol (fluorescing only in hydrophobic environment), (2) from the spirochete incubated 2 h in a medium containing 10 µCi/L tritiated cholesterol, (3) from HeLa cells incubated with the spirochete itself charged with tritiated cholesterol (after removal of all spirochetes). The same analysis was applied (4) to the purified outer membrane vesicles of the spirochete previously charged with BODIPY-cholesterol. In cases (1) and (4), UV detection showed that the BODIPY-cholesterol is incorporated into the cholesterol glycolipids of B. burgdorferi, whereas without incubation it has the same hRf as cholesterol. In cases (1), (2) and (4), iodine staining made free cholesterol and cholesterol-glycolipids (galactopyranosides) visible, but no other (cholesterol-free) lipids. In case (2), the chromatogram was sprayed with EN3HANCE Spray (DuPont), exposed using BioMax MR Film (Kodak) for 4 and 14 days at −80 °C and developed using a Medical Film Processor Model SRX-101A (Konica); the radiolabelled cholesterol was shown incorporated into the cholesterol-glycolipids. In cases (2) and (3), the radiolabelled zones on the layer were scraped off and analyzed by liquid scintillation counting; cholesterol-glycolipids were found (more than free cholesterol) transferred from the spirochete to the HeLa plasmalemma.