Anal. Biochem. 239, 115-117 (1996). TLC of sterols on silica gel with hexane - ethyl ether - acetic acid 80:15:1. Dried plates were rehydrated for 30 sec in PBS and then incubated for 30 min in filipin suspension at 37°C in the dark. Afterwards washed 2 x 2 min in water. Spots visualized at 365 nm. Detection limit 5 ng cholesterol.

J. Chromatogr. A 1026 (1-2), 301-304 (2004) A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100 % depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected by TLC. Detection by fluorescence measurement, quantification limit was 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

J. Chinese Herb Med. 19, 332 (1988) (Zhongcaoyao). TLC of bile acids on silica with isooctane - ether - acetic acid - butanol - water 10:5:5:3:1. Detection by spraying with 30% sulfuric acid and heating at 105°C for 10 min. and observing under UV 365 nm. Fingerprint identification by comparing with standards.

I. J. Liq. Chromatogr. & Rel. Technol. 26, 1095-1108 (2003). TLC of 7 (cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, glycocholic acid, glycodeoxycholic acid, glycolithocholic acid) on silica gel with n-heptane - ethyl acetate -acetic acid 25:20:8. Evaluation with a 10% solution of sulfuric acid in water as visualizing reagent. The spots were developed by heating at 120°C for 20 min. The retardation factor, the separation factors, resolution factors, as well as the constants of pair separation indicated the mobile phase mentioned is the best of 8 mixtures investigated.

Anal. Bioanal. Chem. 391, 2219-2225 (2008). HPTLC/TLC of testosterone and derivates (methyltestosterone, testosterone propionate, testosterone isobutyrate, testosterone phenylpropionate, testosterone isocaproate, testosterone enanthate, testosterone caprate on silica gel, RP-18W and aluminium oxide with a whole range of binary mixtures such as methanol/water, acetonitrile/water, methanol/dichloromethane and acetone/hexane 0 – 100 % on the effect of temperature ranging from -20 to +60 °C under saturated and unsaturated chamber conditions. Detection by dipping in 10 % methanolic phosphomolybdic acid, followed by heating at 100 °C for 10 min. Evaluation under UV 366 and 254 nm. The best separation was observed on RP-18W with methanol/water mobile phases.

J. Chromatogr. 658, 31-53 (1994). Review with 116 references on chromatographic procedures for phytoecdysteroids, including TLC. List of 25 solvent systems and a number of spray reagents.

Neighbouring group participation in hypobromous acid addition to 19-substituted 5 alpha-cholest-1-enes and in acid cleavage of their epoxy analogs. Coll. czechoslov. chem. comm. 47, 3062-3076 (1982). TLC of cholestane derivatives on silica with a) heptane-ether 4:1, b) petrol ether - ether - acetone 90:5:5, c) benzene - ether 1:1, 4:1 and 7:3. Quantification after elution.

J. Chromatogr. 257, 157-161 (1983). TLC of various phytoecdysones on silica with the lower layer of chloroform - methanol -water 13:7:4. Detection by UV 254 nm. TLC is used in order to select the solvent system for droplet countercurrent chromatography (DCCC).