J. Planar Chromatogr. 6, 481-486 (1993). One- and two-dimensional TLC of ecdysteroids (2-deoxyecdysone, 2-deoxy-20-hydroxyecdysone, 20-hydroxyecdysone 22-acetate, 20-hydroxyecdysone, integristerone) with 7 mobile phases and eleven different sets of conditions. Visualization under UV 254 nm.

J. High Resol. Chromatogr. 5, 106-107 (1982). HPTLC of 12 derivatives of transdecalin and 5 alpha-cholestane on silica with a) hexane - benzene 70:30, b) benzene. Detection by UV 254 nm.

Occasional publication No. 6, University of Salford, Salford MS 4 WT, UK (on microfiches 1-3) (1983). Includes 75 references devoted to TLC.

Phytochemistry 23, 73-82 (1984). TLC of Zea mays sterols on silica or silica containing 10 % of silver nitrate with a) chloroform - ether 49:1, b) chloroform - ether 97:3. Detection by UV after spraying with 0.005 % berberine HCl in ethanol. Characterization by HPLC and GC/MS.

(Japanese). Bunseki 9, 647-656 (1988). A review with 12 references.

J. Planar Chromatogr. 4, 255-257 (1991). HPTLC of progesterone, testosterone, testosterone hydrogen sulfate sodium salt, 20ß-hydroxy-4-pregnene-3-one, 2-methoxy estrone, coprostane, hydrocortisone on silica with a gradient consisting of methanol – ethyl acetate – chloroform – methylene chloride (first inverse gradient program) and of methanol – chloroform (second inverse gradient program). Detection under UV 254 nm. Quantification by densitometry.

J. Chromatogr. 674, 247-353 (1994). HPTLC of anabolic steroids on silica with chloroform - acetone 9:1 in one direction and cyclohexane - ethyl acetate - methanol 117:78:11 in the opposite direction. Detection by spraying with 10% sulfuric acid in methanol and heating for 10 min. at 95 °C. Observation in daylight and under UV 366 nm. Confirmation of the identities by GC-MS.

Chromatographia 63 (7-8), 383-388 (2006). Specific and rapid determination of free cortisol and cortisone in human urine has been achieved by concentration of the urine samples, liquid-liquid extraction of the concentrated samples, TLC of ethanolic extracts on silica gel, location of the steroids under UV light, elution of cortisol and cortisone from sections of the plates with phosphate buffer, and measurement by competitive protein-binding assay. Chicken serum was used as the source of corticosteroid binding globulin, because it binds cortisol and cortisone with similar high affinity. The method combining TLC and competitive protein-binding assay is easy to perform, specific, sensitive, and quite rapid. Free cortisol and cortisone were measured in the urine of male individuals who abstained from water intake for 2 h or who ingested 1 L of water. The results show, for the first time, that short-term water diuresis markedly increases urinary free cortisone excretion, supporting our previous hypothesis that its excretion is positively correlated with urine volume, i.e. with the volume of 24-h urine samples.