J. Planar Chromatogr. 8, 155-156 (1995). HPTLC of the 15S and 15R forms of the title prostaglandin on silica with mixtures of dichloromethane and acetone in ratios ranging from 3:1 to 1:1. Detection by immersion into a phosphomolybdic acid solution (10%) and heating at 105 °C for 10 min. Quantification by densitometry at 750 nm (absorbance). Comparison of HPTLC with HPLC. The author considers HPLC 100fold more expensive than HPTLC.

Prostaglandins Leukotr Essent Fatty Acids 52, 223-227 (1995). TLC of hydroxy derivatives of arachidonic acid (12-hydroxyheptadecatrienoic acid and 12-hydroxyeicosatetraenoic acid), thromboxane B2, prostaglandins and phospholipids on silica with the upper organic phase of ethyl acetate - isooctane - acetic acid - water 11:5:2:10 or hexane - ether - acetic acid 80:30:1. Detection by exposure to iodine vapor. After marking the spots were scraped off and counted for radioactivity in a liquid scintillation counter.

J. Planar Chromatogr. 21, 387-390 (2008). TLC of prostaglandin PGE2 and PGF2a on silica gel and RP-18 in a saturated chamber with methanol - dichloromethane 1:9 and 100 % acetonitrile, respectively. Detection by spraying with phosphomolybdic acid (10 % in methanol) and heating at temperatures ranging from 80 to 100 °C (for silica gel) and below 80 °C (for RP-18). Densitometric evaluation; chromatographic spot quantification, however, was performed manually using the peak height above baseline method.

J. High Resol. Chromatogr. 7, 538-539 (1984). TLC of arachidonic acid metabolites on silica, impregnated with phenylmethylvinyl chlorosilane, using heptane - methyl formate - ether - acetic acid 65:25:10:2 for 8.5 cm, followed by 2nd run with hexane - methyl formate-ether - acetic acid 50:40:10:1 for 11 cm. Detection by spraying with 2 % molybdic acid in ethanol.

J. High Resol. Chromatogr. 7, 593-595 (1984). HPTLC of the title compounds on silica with ethanol - ether - ethyl acetate - chloroform 12:33:22:33. Detection by spraying with 5 % cupric acetate in 15 % aqueous phosphoric acid and heating at 110 °C for 10 minutes. Fluorometry at 366 nm. Sensitivity 1-10 ng.

Chem.) 34, 707-711 (l985). (Japanese) (Determination of higher fatty acids in phospholipid subfractions of human platelets using thin-layer chromatography and gas chromatography.) TLC of phospholipid subfractions on silica with chloroform - methanol acetic acid - formic acid 50:30:4.5:6.5. Detection by spraying with 8-anilinonaphtalenesulfonic acid ammonium salt. Quantification by GC after extraction and methylation. Phospholipids were extracted from silica with chloroform - methanol 1:4 once and methanol twice. Methylation was done with boron trifluoride and methanol. The method is highly reliable.

Comparison of the extrapolated Rm values. J. Chromatogr. 347, 209-218 (1985). Rm determination of a series of prostaglandins in two reversed-phase TLC systems, the mobile phase being an aqueous buffer alone or mixed-with various amounts of methanol or acetone. Discussion of the relationship between chromatographic behaviour and mobile phase composition. The extrapolated Rm values are independent of the nature of the organic solvent

J. Agric. Food Chem. 34, 688-694 (1986). Determination of alfaprostol and metabolites on silica with benzene - dioxane - acetic acid 20:20:1. Detection by spraying with phosphomolybdic acid and heating. Detection of radioactive zones with a radiochromatogram spark chamber.