J. Pharm. Biomed. Anal. 189, 113488 (2020). Various extracts from red alga Plocamium dilatatum (Plocamiaceae), green alga Codium fragile tasmanicum (Codiaceae) and brown algae Carpoglossum confluens (1), Cystophora platylobium (2) and C. retorta (3) (Sargassaceae), Ecklonia radiata (Lessoniaceae), Hormosira banksia (Hormosiraceae), Phyllospora comosa (4) (Seirococcaceae) were separated on HPTLC silica gel with n-hexane – ethyl acetate – acetic acid 70:27:3. Detection A) for antioxidant activity by spraying with methanolic DPPH solution, followed by waiting for 30 min at room temperature; B) for steroids and terpenes with anisaldehyde - sulfuric acid solution, followed by heating for 10 min at 110°C; C) for carbohydrates and polyols with thymol - sulfuric acid, followed by heating for 15-20 min at 120°C. Image-based evaluation in white light and UV 366 nm. The most active bands were also characterized by ATR-FTIR (= attenuated total reflectance – Fourier-transformed infrared) spectroscopy.

Food Chem. 362, 130167 (2021). HPTLC of monosaccharides rhamnose, xylose, arabinose, mannose, glucose, galactose and sucrose in cladodes from Opuntia ficus-indica and Opuntia joconostle on silica gel with acetonitrile - 0.3 % ammonium hydroxide 17:3. Detection by dipping into thymol (0.2 %) and sulfuric acid (5 %) in methanol reagent, followed by heating at 95 °C for 2 min.

Planta Med. 85(2), 154-159 (2019). Two new cycloartane triterpene heterosides (cimiheraclein F and a shengmanol derivative) isolated from Actaea heracleifolia aerial parts (Ranunculaceae) were submitted to acid hydrolysis (HCl 0.4 M in methanol 60 %, 90°C, 2 h). The glycone moieties were purified and developed on TLC silica gel with ethyl acetate – chloroform – methanol – water 3:2:2:1. Detection after spraying with sulfuric acid 10 % in water and heating. The glycones were identified in both cases as L-arabinopyranose by comparison of the RF values (and of specific optical rotation) to a standard.

J. Planar Chromatogr. 33, 489-499 (2020). HPTLC of fructose (1), glucose (2) and sucrose (3) in honey on silica gel with 1-butanol-2-propanol - boric acid - water 3:5:1. Detection by spraying with 2 mL of aniline diphenylamine phosphoric acid reagent, followed by heating at 110 ºC for 10 min. The hRF values for (1) to (3) were 14, 32 and 27, respectively. Linearity was between 250 and 1250 ng/zone for (1) to (3). Intermediate precision was below 7 % (n=3). The LOD and LOQ were 22 and 67 ng for (1), 33 and 100 ng for (2) and 21 and 64 ng for (3), respectively. Average recovery was 100.7 % for (1), 101.4 % for (2) and 104.0 % for (3).

J. Planar Chromatogr. 32, 109-114 (2019). HPTLC of fructose (1), glucose (2), maltose (3), raffinose (4) and sucrose (5) in the roots of the seven Asparagus species A. adscendens, A. racemosus, A. retrofractus, A. officinalis, A. densiflorus, A. falcatus, and A. sprengeri on silica gel with propanol - ethyl acetate - water 6:3:1. Detection by spraying with a methanolic solution of diphenylamine, aniline, and ortho-phosphoric acid, followed by heating at 140 °C for 2-3 min. Quantitative determination by absorbance measurement at 600 nm. The hRF values for (1) to (5) were 55, 50, 31, 11 and 46, respectively. Linearity was between 100 and 500 ng for (1) to (5). The intermediate precision was below 2 % (n=3). The LOD and LOQ were 22 and 66 ng for (1), 13 and 40 ng for (2), 51 and 155 ng for (3), 13 and 39 ng for (4) and 76 and 229 ng for (5), respectively. Average recovery was 97.9 % for (1), 99.9 % for (2), 99.3 % for (3), 98.3 % for (4) and 97.8 % for (5).

J. Chromatogr. A 1110 (1-2), 214-221 (2006). Presentation of a rapid, simple and inexpensive method for the analysis of fructooligosaccharides as feed additives (prebiotics) in complicated biological samples with minimal pre-treatment. TLC of fructooligosaccharides in dietetic products and in samples from intestinal tract of monogastric animals on silica gel (impregnated with sodium acetate) with butanol - ethanol - water 5:3:2 with chamber saturation. Detection by spraying with diphenylamine - aniline - phosphoric acid in acetone. Quantitative determination by absorbance measurement at 370 nm.

Acta Chimica 110, 197-202 (1982). TLC of ferric-fructose (FeCl3), ferric-fructose (FeNO3), ferric-sorbose (FeCl3 ) on silica with butanol - acetone - HCl - acetylacetone - water 60:40:1:0.5:20. Detection with 10 % sulfuric acid and 2 % K4FeCN9.

nauk. SSSR, serija chimicheskaja 2400-2402 (1983). (Russian) (Synthesis of 3,4-di-o-benzyl-1,2,-o- (1-cyano)-ethylidcine-alpha -D-xylopyranose.) TLC of the title compound on silica with a) ether - benzene 1:1, 1:2; b) chloroform - methanol 93:7. Detection with 25 % sulfuric acid.