J. Planar Chromatogr. 33, 489-499 (2020). HPTLC of fructose (1), glucose (2) and sucrose (3) in honey on silica gel with 1-butanol-2-propanol - boric acid - water 3:5:1. Detection by spraying with 2 mL of aniline diphenylamine phosphoric acid reagent, followed by heating at 110 ºC for 10 min. The hRF values for (1) to (3) were 14, 32 and 27, respectively. Linearity was between 250 and 1250 ng/zone for (1) to (3). Intermediate precision was below 7 % (n=3). The LOD and LOQ were 22 and 67 ng for (1), 33 and 100 ng for (2) and 21 and 64 ng for (3), respectively. Average recovery was 100.7 % for (1), 101.4 % for (2) and 104.0 % for (3).

J. Planar Chromatogr. 32, 109-114 (2019). HPTLC of fructose (1), glucose (2), maltose (3), raffinose (4) and sucrose (5) in the roots of the seven Asparagus species A. adscendens, A. racemosus, A. retrofractus, A. officinalis, A. densiflorus, A. falcatus, and A. sprengeri on silica gel with propanol - ethyl acetate - water 6:3:1. Detection by spraying with a methanolic solution of diphenylamine, aniline, and ortho-phosphoric acid, followed by heating at 140 °C for 2-3 min. Quantitative determination by absorbance measurement at 600 nm. The hRF values for (1) to (5) were 55, 50, 31, 11 and 46, respectively. Linearity was between 100 and 500 ng for (1) to (5). The intermediate precision was below 2 % (n=3). The LOD and LOQ were 22 and 66 ng for (1), 13 and 40 ng for (2), 51 and 155 ng for (3), 13 and 39 ng for (4) and 76 and 229 ng for (5), respectively. Average recovery was 97.9 % for (1), 99.9 % for (2), 99.3 % for (3), 98.3 % for (4) and 97.8 % for (5).

J. Chromatogr. A 1110 (1-2), 214-221 (2006). Presentation of a rapid, simple and inexpensive method for the analysis of fructooligosaccharides as feed additives (prebiotics) in complicated biological samples with minimal pre-treatment. TLC of fructooligosaccharides in dietetic products and in samples from intestinal tract of monogastric animals on silica gel (impregnated with sodium acetate) with butanol - ethanol - water 5:3:2 with chamber saturation. Detection by spraying with diphenylamine - aniline - phosphoric acid in acetone. Quantitative determination by absorbance measurement at 370 nm.

Acta Chimica 110, 197-202 (1982). TLC of ferric-fructose (FeCl3), ferric-fructose (FeNO3), ferric-sorbose (FeCl3 ) on silica with butanol - acetone - HCl - acetylacetone - water 60:40:1:0.5:20. Detection with 10 % sulfuric acid and 2 % K4FeCN9.

nauk. SSSR, serija chimicheskaja 2400-2402 (1983). (Russian) (Synthesis of 3,4-di-o-benzyl-1,2,-o- (1-cyano)-ethylidcine-alpha -D-xylopyranose.) TLC of the title compound on silica with a) ether - benzene 1:1, 1:2; b) chloroform - methanol 93:7. Detection with 25 % sulfuric acid.

J.A.O.A.C. 72, 27,29 (1989). TLC separation of polysorbates (20, 40, 60, 65, 80) on silica with chloroform - methanol - acetic acid - water 68:12:12:3, propanol - chloroform - methanol - NH3 10:10:5:2. Visualization with cobalt thiocyanate reagent.

Var. australis (Diels) Rehd.) (Chinese). Chinese J. Herb Med. (Zhongcaoyao) 24, 563-566(1993). Preparative TLC of saponins and monosaccharides on silica with the lower phases of a) chloroform - methanol - water 16:5:1, and b) a solution of 9 mL of the above mixture blended with 1 mL acetic acid. Detection by spraying with 50% sulfuric acid in methanol for saponins and with naphthyl alcohol - sulfuric acid reagent for saccharides. Identification by MS and NMR.

J. Planar Chromatogr. 18, 285-289 (2005). HPTLC of glucose - after hydrolyis of starch using alpha-amylase and amyloglucosidase - on silica gel, pre-washed with methanol, treated by immersion in a 0.1 M solution of di-potassium hydrogen phosphate in methanol and activated for 30 min at 120 °C. Three-fold development was performed in a horizontal development chamber with acetonitrile - water 17:3. Detection by dipping in aniline-diphenylamine reagent, densitometry at 520 nm. Calibration was linear between 100 and 300 ng with a coefficient of determination r2 of 0.9959. The limits of detection and quantification for starch as glucose were 0.26 and 0.51 g/100 g, respectively.