Phytochem. Anal. 35, 163-183 (2024). HPTLC of Cannabis sativa on silica gel with n-heptane - methyl tert-butyl ether - ethanol - formic acid 780:170:50:3. Detection by spraying with vanillin-sulfuric acid (VSA) reagent (5 % methanolic solutions), followed by heating at 120 °C for 3 min. Fast Blue solution (0.5 % in water - methanol - dichloromethane 2:5:3) was used for derivatisation as a selective stain for cannabinoids. A 0.1 M sodium hydroxide solution in methanol - water 9:1 was used to improve color development.

PLoS Neglected Tropical Diseases 17(09), e0011646 (2023). Samples were extracts rich in sphingolipids obtained from Trypanosoma cruzi epimastigotes, or from Leishmania major promastigotes (Trypanosomatidae), or from Chlorocebus sp. kidney Vero cells (Cercopithecidae), all cell lines incubated 2h before the extractions with ceramide N-hexanoyl-D-erythro-sphingosine coupled to fluorescent NBD-amine group (NBD = nitrobenzoxadiazolyle). Dried extracts were resuspended in chloroform – methanol (1:1) before application on TLC silica gel layers. Development with chloroform – methanol – potassium chloride 0.25 % aqueous solution 11:9:2. Visualization under automated laser scanner. Three sphingolipids were detected due to the NBD fluorescent group: sphingomyelin (hRF 42) was present in Vero cells only (negative control), whereas the targeted inositol-phosphorylceramide (IPC, hRF 70), was present in both L. major (positive control) and T. cruzi wild-type. It was absent in T. cruzi cell lines knock-out (KO) for the IPC-synthase (IPCS) gene, but present again in the add-back cell-lines (obtained with plasmide transfection of the IPCS gene into KO cells). An unknown lipid (hRF 78) was detected in all T. cruzi samples.

Phytochem. 214, 113798 (2023). Review of the distribution of artemisinin in underutilized crops of Artemisia genus, including TLC and HPTLC methods for the analysis of different plant species.

PLoS ONE 18(11), e0294775 (2023). Sample was an Azadirachta indica seed extract (Meliaceae). TLC on silica gel with different mobile phases: (1) diethyl ether – methanol 49:1; (2) diethyl ether – acetone 2:1; (3) isopropanol – n-hexane 11:9; (4) dichloromethane – methanol – acetic acid 95:5:1. After 30 min hot air drying,detection under UV light. The hRF values of azadirachtin A (a limonoid) were 75, 42, 44, 55, respectively. Mobile phase (1) was therefore chosen as solvent for purification of azadirachtin A and for its quantification by Fourier transform infrared spectroscopy (FTIR).

PLoS ONE 18(11), e0295012 (2023). TLC on silica gel to monitor the synthesis of 15 new camphene-based thiosemicarbazones produced by the reaction of camphene thiosemicarbazide either with benzaldehydes, or with acetophenones, or with one of the following molecules: benzophenone, cinnamic aldehyde, ethyl pyruvate, furaldehyde, menthone, pyrrole carboxaldehyde or thiophene-carboxaldehyde. Development with n-hexane – ethyl acetate 3:7 in the case of benzaldehydes, except vanillin; or 7:3 for the vanillin derivative and all others, followed by visualization of products with resublimated iodine. The aldehyde used for compound 15 is in fact vanillin.

J. Planar Chromatogr. 36, 121-127 (2023). HPTLC of genistein in Flemingia procumbens on silica gel with toluene - ethyl acetate - acetone - formic acid 20:4:2:1. Quantitative determination by absorbance measurement at 271 nm. The hRF value for genistein was 49. Linearity was in the range of 50-300 ng/zone. Intermediate precisions were below 2 % (n=6). LOD and LOQ were 2 and 71 ng/zone, respectively. Average recovery was 99.6 %.

J. Planar Chromatogr. 36, 157-167 (2023). HPTLC of mangiferin in Mangifera indica on silica gel with acetone - chloroform - formic acid - water 4:20:1:2. Quantitative determination by absorbance measurement at 350 nm. The hRF values for mangiferin was 54. Linearity was in the range of 0.2-1.0 μg/zone. Intermediate precisions were below 1 % (n=3). LOD and LOQ were 64 and 210 ng/zone, respectively. Average recovery was 100 %.

J. Planar Chromatogr. 36, 169-178 (2023). HPTLC of ellagic acid (1) and gallic acid (2) in Terminalia species (Terminalia arjuna, T. bellirica, T. chebula, and T. catappa) on silica gel with methanol - water - formic acid - acetic acid 20:25:1:1. Quantitative determination by absorbance measurement at 279 and 254 nm. The hRF values for (1) and (2) were 33 and 82, respectively. Linearity was in the range of 100 and 700 ng/zone. Intermediate precisions were below 1 % (n=3). LOD and LOQ were 80 and 240 ng/zone for (1) and 75 and 225 ng/zone for (2). Average recovery was between 95.5 and 98.4 % for (1) and (2).