J. Planar Chromatogr. 24, 423-427 (2011). HPTLC of salmeterol xinafoate (as salmeterol base SAL and xinafoic acid XIN) on silica gel with ethyl acetate - methanol - 33 % ammonia 16:3:1 with chamber saturation for 1 h. Detection under UV light at 254 nm. Quantitative determination by densitometry at 300 nm (SAL) and at 250 nm (XIN). The hRf value was 48 for SAL and 36 for XIN. Linearity was betwen 1-6 µg/zone for SAL and 0.5-4 µg/zone for XIN. The recovery (by standard addition) was 100.6 % for SAL and 99.8 % for XIN. The intra-day and inter-day precision, as %RSD, was 0.7 and 1.1 % for SAL, and 0.9 and 1.0 % for XIN. The limit of detection and of quantification was 0.4 and 1.2 µg/zone for SAL and 0.1 and 0.3 µg/zone for XIN.

Planta Med. 83, 1035-1043 (2017). TLC on silica gel with detection with anisaldehyde or Kedde’s reagent, 1) with ethyl acetate to monitor the isolation of digitoxin, by flash chromatography, from a fraction of a methanolic extract of Digitalis lanata, 2) with ethyl acetate – dichloromethane 1:1 to monitor five different methods for the hemisynthesis (with silver oxyde and diphenylborinic acid) of glucoevatromonoside from evatromonoside, itself produced from digitoxin, 3) with ketone – methanol 19:1 to analyse the glucoevatromonoside produced by biotransformation of evatromonoside by the glycosyltransferase activity of suspension cell cultures of Digitalis lanata. Digitoxigenin was used as internal standard, using the methanol fraction of the cell lysates, either directly or after a purification by flash chromatography. Hemisynthesis was twice more efficient as biotransformation, but the yield did not reach 70%.

J. Planar Chromatogr. 5, 239-245 (1992). Study of the HPTLC behavior of closely related diethanolamines on silica developed with 4 binary eluents and evaluation of the effect of solute - solvent association using the thermodynamically formulated Oscik criterion.

J. AOAC Int. 93, 792-797 (2010). HPTLC of alprenolol on silica gel with methanol - 25 % ammonia 99:1 in a chamber saturated for 10 min. Quantitative determination by absorbance measurement at 270 nm. The linearity range was between 1.03 and 20.6 µg/zone. The %RSD of precision was 3.9 %. The recovery of alprenolol was at 80 % level 100.1 % with a %RSD of 2.2 %, at 100 % level 99.9 % with a %RSD of 3.9 %, and at 120 % level 104.4 % with a %RSD of 2.9 %. The LOD was 520 ng/zone and LOQ 1550 ng/zone.

Planta Med. 83, 1097-1102 (2017). The fractionations on a silica gel column (with a gradient of ethyl acetate, methanol and petroleum ether/dichloromethane) of the dichloromethane and methanol extracts of Antidesma ghaesembilla bark were monitored by TLC on silica gel with chloroform – methanol – water – formic acid 290:20:1:1 or 290:50:1:1, respectively. From fractions of the dichloromethane extract, chavibetol, sitostenone, asperphenamate, daucosterol, 5,7-dimethoxy-aristolochic acid II and 10-amino-5,7-dimethoxy-aristolic acid II were later isolated, the last one was detected by Dragendorff reagent. From fractions of the methanol extract, glucosides of aristolic acid and of vanillic acid were isolated and separated by preparative TLC with chloroform – methanol – acetone – formic acid 450:75:25:2. This preparative TLC yielded protocatechuic acid (hRF 51) and a mixture (hRF 14–19), from which a methyl-phloroglucinol glucoside was isolated by gel exclusion chromatography.

J. Planar Chromatogr. 5, 282-283 (1992). TLC of 4-methylbenzyl alcohol, 2-phenylethanol, 1-phenyl-1-ethanol, myristyl alcohol, isoborneol, menthol, cetyl and stearyl alcohol on silica with hexane - acetone 8:2. Detection with thymol blue and bromothymol blue (each dissolved in aqueous sodium hydroxide solution, 2 %, 100 mL), evaluation 10 min. after spraying, then heating at 110 °C for 10 min. and re-examination. Detection limit: between 1.5 and 12 µg.

Journal of Pharmacy Research 4(5), 1401-1404 (2011). HPTLC of fluconazole and its structurally related impurities, on silica gel (prewashed with methanol) with n-butanol - water - acetic acid 8:2:1 with chamber saturation for 20 min. The hRf value of fluconazole was 67 and of the two separated impurities 49 (impurity B) and 79 (impurity E). Quantitative determination by densitometry in absorbance mode at 254 nm. The method was linear in the range of 1-6 µg/band for fluconazole and 0.5-2.5 µg/band for both impurities.

Apoth. Ztg. 125, 1163-1166 (1985). Zum Nachweis des Ethanols als 3,5-Dinitrobenzoesäureethylester. (Determination of ethanol as 3,5-dinitrobenzoic acid ethyl ester). Determination of ethanol, methanol, propanol, isopropanol, butanol as 3,5-dinitrobenzoic acid esters on RP-18 silica with methanol - dioxane - water 3:1:1. Detection by UV.